1. Cell Cycle/DNA Damage
    Epigenetics
    Autophagy
  2. Sirtuin
    Autophagy
  3. Sirtinol

Sirtinol 

Cat. No.: HY-13515 Purity: >98.0%
Handling Instructions

Sirtinol is a sirtuin inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.

For research use only. We do not sell to patients.

Sirtinol Chemical Structure

Sirtinol Chemical Structure

CAS No. : 410536-97-9

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
5 mg USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 102 In-stock
Estimated Time of Arrival: December 31
50 mg USD 408 In-stock
Estimated Time of Arrival: December 31
100 mg USD 696 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Sirtinol purchased from MCE. Usage Cited in: Mol Cell Biochem. 2018 Feb;439(1-2):213-223.

    Western blot analysis in cells treated with 10 μM Sirtinol prior to incubation with 200 μM Resveratrol (RES) calculated from density values of specific protein bands/GAPDH density values and expressed as a percentage of the control.

    Sirtinol purchased from MCE. Usage Cited in: Cell Death Dis. 2018 May 1;9(5):559.

    The inhibitory effect of CHES1 on transcription activity of ERα Is diminished in MCF7 cells when treated with Sirtinol (the SIRT1 inhibitor).

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    Description

    Sirtinol is a sirtuin inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.

    IC50 & Target[4]

    ySir2

    48 μM (IC50)

    hSIRT2

    57.7 μM (IC50)

    hSIRT1

    131 μM (IC50)

    In Vitro

    Sirtinol reduces the growth of MCF-7 cells in a concentration- and time-dependent manner. The IC50 values of sirtinol are 48.6 μM and 43.5 μM after 24 and 48 h of treatment, respectively. Sirtinol significantly decreases SIRT1 expression and increases the acetylated p53 level[1]. Sirtinol attenuates the proliferation and induces apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells and causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1[2].

    In Vivo

    Sirtinol has anti-inflammatory effects through direct inhibition of HNE activity and attenuates HNE-induced and LPS-mediated tissue or organ injury[3].

    Molecular Weight

    394.47

    Formula

    C₂₆H₂₂N₂O₂

    CAS No.

    410536-97-9

    SMILES

    O=C(NC(C1=CC=CC=C1)C)C2=CC=CC=C2/N=C/C3=C4C=CC=CC4=CC=C3O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 6.5 mg/mL (16.48 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5350 mL 12.6752 mL 25.3505 mL
    5 mM 0.5070 mL 2.5350 mL 5.0701 mL
    10 mM 0.2535 mL 1.2675 mL 2.5350 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [2]

    Sirtinol is dissolved in 100% DMSO at concentration of 10 mM and stored at −20°C until use. The cell proliferation of H1299 cells is determined by trypan blue dye exclusion assay. Human nonsmall cell lung cancer (NSCLC) cells are seeded in 12-well plates and treated with indicated concentrations of sirtinol (0, 10, 20, and 50 μM) for 24 h and 48 h, respectively. After incubation, the cells are stained by 0.2% trypan blue and counted by Countess[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Mice: 30 male mice (20–25 g, 7–8 weeks old) are used in this model. Briefly, mice are randomly divided into five groups; then mice are intraperitoneally injected with 50 μL DMSO (vehicle group) or sirtinol (2.5 or 5.0 mg/kg body weight). After 1 h, paw inflammation is induced . The thickness of the paw is measured before and after HNE or saline injection[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Sirtinol
    Cat. No.:
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