2. Cell Cycle/DNA Damage Epigenetics Autophagy Apoptosis
  3. Sirtuin Autophagy Apoptosis
  4. Sirtinol

Sirtinol 

Cat. No.: HY-13515 Purity: ≥98.0%
COA Handling Instructions

Sirtinol is a sirtuin (SIRT) inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.

For research use only. We do not sell to patients.

Sirtinol Chemical Structure

Sirtinol Chemical Structure

CAS No. : 410536-97-9

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Solution
10 mM * 1 mL in DMSO USD 80 In-stock
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10 mM * 1 mL
ready for reconstitution
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Solid
5 mg USD 73 In-stock
10 mg USD 112 In-stock
50 mg USD 449 In-stock
100 mg USD 766 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 14 publication(s) in Google Scholar

Sirtinol Related Antibodies

Top Publications Citing Use of Products

    Sirtinol purchased from MCE. Usage Cited in: Cell Death Dis. 2018 May 1;9(5):559.  [Abstract]

    The inhibitory effect of CHES1 on transcription activity of ERα Is diminished in MCF7 cells when treated with Sirtinol (the SIRT1 inhibitor).

    Sirtinol purchased from MCE. Usage Cited in: Mol Cell Biochem. 2018 Feb;439(1-2):213-223.  [Abstract]

    Western blot analysis in cells treated with 10 μM Sirtinol prior to incubation with 200 μM Resveratrol (RES) calculated from density values of specific protein bands/GAPDH density values and expressed as a percentage of the control.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Sirtinol is a sirtuin (SIRT) inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively[1][2][3][4].

    IC50 & Target[4]

    ySir2

    48 μM (IC50)

    hSIRT2

    57.7 μM (IC50)

    hSIRT1

    131 μM (IC50)

    In Vitro

    Sirtinol reduces the growth of MCF-7 cells in a concentration- and time-dependent manner. The IC50 values of sirtinol are 48.6 μM and 43.5 μM after 24 and 48 h of treatment, respectively. Sirtinol significantly decreases SIRT1 expression and increases the acetylated p53 level[1]. Sirtinol attenuates the proliferation and induces apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells and causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Sirtinol Related Antibodies
    In Vivo

    Sirtinol has anti-inflammatory effects through direct inhibition of HNE activity and attenuates HNE-induced and LPS-mediated tissue or organ injury[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    394.47

    Appearance

    Solid

    Formula

    C26H22N2O2
    CAS No.
    SMILES

    O=C(NC(C1=CC=CC=C1)C)C2=CC=CC=C2/N=C/C3=C4C=CC=CC4=CC=C3O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (25.35 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5350 mL 12.6752 mL 25.3505 mL
    5 mM 0.5070 mL 2.5350 mL 5.0701 mL
    10 mM 0.2535 mL 1.2675 mL 2.5350 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 1 mg/mL (2.54 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
    Cell Assay
    [2]

    Sirtinol is dissolved in 100% DMSO at concentration of 10 mM and stored at −20°C until use. The cell proliferation of H1299 cells is determined by trypan blue dye exclusion assay. Human nonsmall cell lung cancer (NSCLC) cells are seeded in 12-well plates and treated with indicated concentrations of sirtinol (0, 10, 20, and 50 μM) for 24 h and 48 h, respectively. After incubation, the cells are stained by 0.2% trypan blue and counted by Countess[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Mice: 30 male mice (20–25 g, 7–8 weeks old) are used in this model. Briefly, mice are randomly divided into five groups; then mice are intraperitoneally injected with 50 μL DMSO (vehicle group) or sirtinol (2.5 or 5.0 mg/kg body weight). After 1 h, paw inflammation is induced . The thickness of the paw is measured before and after HNE or saline injection[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Sirtinol Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Sirtinol410536-97-9SirtuinAutophagyApoptosisInhibitorinhibitorinhibit

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