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ApoE Antibody (YA3620)

(Synonyms: AD2; LPG; LDLCQ5; MGC1571)
Customer Review

Based on 1 Customer Validation

ApoE Antibody (YA3620) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ApoE.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • ApoE Antibody (YA3620) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

ApoE Antibody (YA3620) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ApoE.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Immunogen

Purified recombinant fragment of human ApoE aa 20-267.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718796

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ApoE Antibody (YA3620)
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    Immunohistochemical analysis of paraffin-embedded Human liver tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    Immunohistochemical analysis of paraffin-embedded Human testis tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    Immunohistochemical analysis of paraffin-embedded Human Glioma tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    Flow Cytometry analysis of HepG2 cells labelling ApoE (red) with ApoE Antibody (anti-ApoE) (HY-P80757). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control(HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Apolipoprotein E (ApoE) is a key regulator of lipoprotein metabolism that mediates the hepatic clearance of diet-derived chylomicron remnants and liver-derived very-low-density lipoprotein (VLDL) remnants through interactions with members of the low-density lipoprotein receptor family[1][2]. ApoE deficiency disrupts this clearance pathway, resulting in marked hypercholesterolemia, accumulation of remnant lipoproteins, and enhanced susceptibility to atherosclerotic lesion formation[1][3][4]. Mechanistically, ApoE regulates cholesterol homeostasis and influences macrophage biology, linking lipid metabolism to vascular inflammation and atherogenesis[2][5]. In disease models, Apoe−/− mice develop spontaneous atherosclerosis even when maintained on a low-cholesterol diet and therefore represent one of the most widely used experimental systems for investigating cardiovascular disease mechanisms and therapeutic interventions[3][4][6]. Atherosclerotic lesions in these mice progress in a manner that reproduces many pathological features of human disease, making the model highly valuable for studies of plaque development and progression[6][2]. Compared with related human APOE isoforms, ApoE deficiency represents a complete loss-of-function state rather than an isoform-specific alteration, providing a robust platform for dissecting the physiological roles of ApoE in lipoprotein transport, monocyte/macrophage biology, and atherosclerosis[2][5]. For experimental applications, the Apoe−/− model is extensively used to evaluate genetic, nutritional, and pharmacological factors that modify atherosclerotic burden and vascular inflammation[6][2].

  • Subcellular Localization

    Secreted; Secreted, extracellular space; Secreted, extracellular space, extracellular matrix; Extracellular vesicle; Endosome, multivesicular body

  • Expression


    Tissue_specificity:Produced by various tissue and cell types, it is mainly found in lipid particles in plasma, interstitial fluid, and lymph (PubMed:25173806) . It is primarily synthesized by hepatocytes (PubMed:25173806) . It is also produced in large quantities in brain tissue, mainly by astrocytes and glial cells in the cerebral cortex, and also by neurons in the frontal cortex and hippocampus (PubMed:10027417, PubMed:3115992) . Cells in the peripheral nervous system also express it (PubMed:10027417, PubMed:25173806) . It is also expressed in the adrenal glands, testes, ovaries, skin, kidneys, spleen, adipose tissue, and macrophages in various tissues (PubMed:25173806) .

  • Subunit

    Homotetramer (PubMed:8340399). May interact with ABCA1; functionally associated with ABCA1 in the biogenesis of HDLs (PubMed:14754908). May interact with APP/A4 amyloid-beta peptide; the interaction is extremely stable in vitro but its physiological significance is unclear (PubMed:23620513, PubMed:8367470). May interact with MAPT (PubMed:7972031). May interact with MAP2 (PubMed:7891887). In the cerebrospinal fluid, interacts with secreted SORL1 (PubMed:30448281). Interacts with PMEL; this allows the loading of PMEL luminal fragment on ILVs to induce fibril nucleation

  • SwissProt ID

    P02649

  • Gene ID
    348 [NCBI]
  • Synonyms

    AD2; LPG; LDLCQ5; MGC1571

[1]. Pendse AA, et al. Apolipoprotein E knock-out and knock-in mice: atherosclerosis, metabolic syndrome, and beyond. J Lipid Res. 2009 Apr;50 Suppl(Suppl):S178-82. [Content Brief]

[2]. Getz GS, et al. ApoE knockout and knockin mice: the history of their contribution to the understanding of atherogenesis. J Lipid Res. 2016 May;57(5):758-66.

[3]. Buzello M, et al. The apolipoprotein e knockout mouse: a model documenting accelerated atherogenesis in uremia. J Am Soc Nephrol. 2003 Feb;14(2):311-6. [Content Brief]

[4]. Lo Sasso G, et al. The Apoe(-/-) mouse model: a suitable model to study cardiovascular and respiratory diseases in the context of cigarette smoke exposure and harm reduction. J Transl Med. 2016 May 20;14(1):146. [Content Brief]

[5]. Sciencedirect.topics.

[6]. Meir KS, et al. Atherosclerosis in the apolipoprotein-E-deficient mouse: a decade of progress. Arterioscler Thromb Vasc Biol. 2004 Jun;24(6):1006-14. [Content Brief]

[7]. Getz GS, et al. ApoE knockout and knockin mice: the history of their contribution to the understanding of atherogenesis. J Lipid Res. 2016 May;57(5):758-66. [Content Brief]

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ApoE Antibody (YA3620) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human placenta tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human testis tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human Glioma tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow Cytometry analysis of HepG2 cells labelling ApoE (red) with ApoE Antibody (anti-ApoE) (HY-P80757). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control(HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ApoE Antibody (YA3620) Product Image
    1/16
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • ApoE Antibody (YA3620) Product Image
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human placenta tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human testis tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human Glioma tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ApoE Antibody (YA3620)
    1/1
    MedChemExpress Validation
     Flow Cytometry analysis of HepG2 cells labelling ApoE (red) with ApoE Antibody (anti-ApoE) (HY-P80757). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control(HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • Experimental Validation Results for ApoE Antibody (YA3620)
  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human liver tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human testis tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human Glioma tissue using ApoE Antibody (HY-P83923, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ApoE Antibody (YA3620) Product Image
    MedChemExpress Validation

    Flow Cytometry analysis of HepG2 cells labelling ApoE (red) with ApoE Antibody (anti-ApoE) (HY-P80757). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control(HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ApoE Antibody (YA3620) Product Image IHC-P
  • ApoE Antibody (YA3620) Product Image IHC-P
  • ApoE Antibody (YA3620) Product Image IHC-P
  • ApoE Antibody (YA3620) Product Image IHC-P
  • ApoE Antibody (YA3620) Product Image IHC-P
  • ApoE Antibody (YA3620) Product Image IHC-P
  • ApoE Antibody (YA3620) Product Image FC