CXCR2 Antibody (YA3904)

(Synonyms: CXCR2; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b)
Customer Review

Based on 1 Customer Validation

CXCR2 Antibody (YA3904) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CXCR2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:100 1:200-1:400 1:10000

Product Details

Description

CXCR2 Antibody (YA3904) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CXCR2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 41 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Immunogen

Purified recombinant fragment of human CD182 expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human colon cancer using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human bladder cancer using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human breast cancer using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human liver using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human prostate using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CXCR2 Antibody (YA3904)
    Immunohistochemical analysis of paraffin-embedded human kidney using CXCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84207, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CXCR2 is a G protein-coupled chemokine receptor that predominantly regulates neutrophil recruitment, leukocyte trafficking, and inflammatory responses through binding multiple ELR+ CXC chemokines, including CXCL1 and CXCL8/IL-8[1][2]. Mechanistically, CXCR2 activation triggers chemokine-dependent signaling pathways that promote integrin activation, cell migration, adhesion, and tissue infiltration, thereby coordinating innate immune responses during infection and tissue injury[1]. Beyond physiological inflammation, the CXCL-CXCR2 signaling axis contributes to angiogenesis, tumor cell migration, invasion, and stromal remodeling in multiple cancer types, including pancreatic, colorectal, breast, lung, and ovarian cancers[3][4][5]. In disease models, elevated CXCR2 signaling promotes recruitment of neutrophils and myeloid-derived suppressor cells, supporting tumor progression and immunosuppressive microenvironments[3][4]. Compared with the related receptor CXCR1, which shares CXCL8 responsiveness, CXCR2 exhibits broader ligand recognition and mediates diverse inflammatory and tumor-associated chemokine networks across immune, endothelial, epithelial, and stromal cells[2][5]. Therefore, CXCR2 has emerged as a major experimental and therapeutic target, and multiple CXCR2 antagonists have demonstrated activity in preclinical cancer, cardiovascular, lung injury, and inflammatory disease models by reducing leukocyte recruitment, angiogenesis, and pathological inflammation[1][2][3].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Subunit

    Interacts with IL8 (PubMed:1891716). Interacts with GNAI2 (PubMed:8662698)

  • SwissProt ID

    P25025

  • Gene ID
  • Synonyms

    CXCR2; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b

CXCR2 Antibody (YA3904) Related Classifications

MOQ
Minimum order quantity
100 mg

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