1. Apoptosis
  2. TNF Receptor
  3. Benpyrine

Benpyrine 

Cat. No.: HY-133807
Handling Instructions

Benpyrine is a highly specific and orally active TNF-α inhibitor with a KD value of 82.1 μM. Benpyrine tightly binds to TNF-α and blocks its interaction with TNFR1, with an IC50 value of 0.109 µM. Benpyrine has the potential for TNF-α mediated inflammatory and autoimmune disease research.

For research use only. We do not sell to patients.

Benpyrine Chemical Structure

Benpyrine Chemical Structure

CAS No. : 2550398-89-3

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10 mM * 1 mL in DMSO USD 440 In-stock
Estimated Time of Arrival: December 31
5 mg USD 400 In-stock
Estimated Time of Arrival: December 31
10 mg USD 680 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1350 In-stock
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50 mg USD 2200 In-stock
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100 mg USD 3400 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Benpyrine is a highly specific and orally active TNF-α inhibitor with a KD value of 82.1 μM. Benpyrine tightly binds to TNF-α and blocks its interaction with TNFR1, with an IC50 value of 0.109 µM. Benpyrine has the potential for TNF-α mediated inflammatory and autoimmune disease research[1].

IC50 & Target[1]

TNF-α

82.1 μM (Kd)

In Vitro

Benpyrine (5-20 µM; 14 hours; RAW264.7 cells) pretreatment results in a dose-dependent decrease in the phosphorylation of IκBα in RAW264.7 cells (stimulated with 10 ng/mL TNF-α or 1 μg/mL LPS). Benpyrine abolishes the TNF-α-induced nuclear translocation of NF-κB/p65 in RAW264.7 cells[1].
Benpyrine only blocks cell death induced by TNF-αWT and Y119A, and increases the cell survival rate up to 80%. Benpyrine does not obviously affect L57A- and Y59L-induced cytotoxicity in L929 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: RAW264.7 cells
Concentration: 5 µM, 10 µM, 20 µM
Incubation Time: 14 hours
Result: Resulted in a dose-dependent decrease in the phosphorylation of IκBα.
In Vivo

Benpyrine (25-50 mg/kg; oral gavage; daily; for 2 weeks; Balb/c mice) treatment significantly relieves the symptoms of collagen-induced arthritis. Benpyrine dose-dependently decreases the levels of proinflammatory cytokines, such as IFN-γ, IL-1β and IL-6, and increases the concentration of the anti-inflammatory cytokine IL-10[1].
Endotoxemia murine model shows that Benpyrine (25 mg/kg) could attenuate TNF-α-induced inflammation, thereby reducing liver and lung injury[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c mice (18-20 g) injected with collagen[1]
Dosage: 25 mg/kg, 50 mg/kg
Administration: Oral gavage; daily; for 2 weeks
Result: Relieved the symptoms of collagen-induced arthritis in mice.
Molecular Weight

308.34

Formula

C₁₆H₁₆N₆O

CAS No.

2550398-89-3

SMILES

O=C(N(CC1=CC=CC=C1)C2)C[[email protected]@H]2NC3=C4C(NC=N4)=NC=N3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

BenpyrineTNF ReceptorTumor Necrosis Factor ReceptorTNFRTNF-αorallyanti-inflammatoryautoimmunep-IκBαNF-κBnuclearIL-10IFN-γIL-1βIL-6TNFR1Inhibitorinhibitorinhibit

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