Benzohydroxamic acid
Based on 1 Customer Validation
Benzohydroxamic acid (BHA) is a chitin deacetylase (CDA) inhibitor with significant antifungal activity. The Ki values of BHA against the CDAs of Verticillium dahliae and Puccinia striiformis f. sp. tritici are 8.31 μM and 9.83 μM, respectively. Benzohydroxamic acid can restore the defense responses of infected host plants, upregulate the expression of defense-related genes, and reduce the growth and reproduction of fungi in plants. Benzohydroxamic acid can be used in the research of the field of controlling agricultural fungal diseases, such as various plant fungal diseases caused by Verticillium dahliae, Puccinia striiformis and other fungi, like cotton wilt and wheat stripe rust .
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- Purity: 99.95%
- CAS No.: 495-18-1
- 화학식: C7H7NO2
- 분자량:137.14
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보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-231 | IC50 |
9.85 μM
Compound: 7a
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Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell proliferation after 48 hrs in presence of salinomycin by MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell proliferation after 48 hrs in presence of salinomycin by MTT assay
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[PMID: 28262526] |
| RBL-1 | IC50 |
110 μM
Compound: 1
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In vitro inhibitory activity against RBL-1 5-LO
In vitro inhibitory activity against RBL-1 5-LO
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[PMID: 3820229] |
| RBL-1 | IC50 |
110 μM
Compound: 10
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In vitro inhibitory activity against 5-lipoxygenase in rat basophilic leukemia cells(RBL-1)
In vitro inhibitory activity against 5-lipoxygenase in rat basophilic leukemia cells(RBL-1)
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[PMID: 2308149] |
| Sf9 | IC50 |
2370 nM
Compound: 1
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Inhibition of recombinant full length human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 insect cells pretreated with compound followed by Fluor de Lys deacetylase substrate addition by fluorescence method
Inhibition of recombinant full length human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 insect cells pretreated with compound followed by Fluor de Lys deacetylase substrate addition by fluorescence method
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[PMID: 31710483] |
| Sf9 | IC50 |
71572 nM
Compound: 1
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Inhibition of C-terminal His-tagged human HDAC3 (1 to 428 residues)/N-terminal GST tagged human NCOR2 (395 to 489) expressed in baculovirus infected sf9 insect cells pretreated with compound followed by Fluor de Lys deacetylase substrate addition by fluor
Inhibition of C-terminal His-tagged human HDAC3 (1 to 428 residues)/N-terminal GST tagged human NCOR2 (395 to 489) expressed in baculovirus infected sf9 insect cells pretreated with compound followed by Fluor de Lys deacetylase substrate addition by fluor
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[PMID: 31710483] |
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 495-18-1
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Appearance Solid
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분자량 137.14
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화학식 C7H7NO2
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Color White to off-white
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SMILES
O=C(NO)C1=CC=CC=C1
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Synonyms
BHA
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
DMSO : 100 mg/mL (729.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (36.46 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (36.46 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.2918 mL | 36.4591 mL | 72.9182 mL | 182.2955 mL |
| 5 mM | 1.4584 mL | 7.2918 mL | 14.5836 mL | 36.4591 mL | |
| 10 mM | 0.7292 mL | 3.6459 mL | 7.2918 mL | 18.2295 mL | |
| 15 mM | 0.4861 mL | 2.4306 mL | 4.8612 mL | 12.1530 mL | |
| 20 mM | 0.3646 mL | 1.8230 mL | 3.6459 mL | 9.1148 mL | |
| 25 mM | 0.2917 mL | 1.4584 mL | 2.9167 mL | 7.2918 mL | |
| 30 mM | 0.2431 mL | 1.2153 mL | 2.4306 mL | 6.0765 mL | |
| 40 mM | 0.1823 mL | 0.9115 mL | 1.8230 mL | 4.5574 mL | |
| 50 mM | 0.1458 mL | 0.7292 mL | 1.4584 mL | 3.6459 mL | |
| 60 mM | 0.1215 mL | 0.6077 mL | 1.2153 mL | 3.0383 mL | |
| 80 mM | 0.0911 mL | 0.4557 mL | 0.9115 mL | 2.2787 mL | |
| 100 mM | 0.0729 mL | 0.3646 mL | 0.7292 mL | 1.8230 mL |
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