Art. -Nr.: HY-147348

4-Formylcolchicine

Name: 4-Formylcolchicine
Brand: MedChemExpress
SKU: HY-147348
Price: 95.00 USD
Availability: InStock
Rating: 5 (1 reviews)

Kundenbewertung

Based on 1 Customer Validation

4-Formylcolchicine is a potent anticancer agent. 4-Formylcolchicine shows cytotoxicity activity with IC₅₀ values of 1.007, 0.128, 0.054 μM for A549, HT-29, HCT116 cells, respectively.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.84%
CAS. Nr.: 2730-82-7
Formel: C₂₃H₂₅NO₇
Molecular Weight:427.45

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

4-Formylcolchicine Chemical Structure

CAS. Nr.: 2730-82-7

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

4-Formylcolchicine is a potent anticancer agent. 4-Formylcolchicine shows cytotoxicity activity with IC₅₀ values of 1.007, 0.128, 0.054 μM for A549, HT-29, HCT116 cells, respectively^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.007 μM Compound: 7	Cytotoxicity against human A549 cells after 96 hrs using tetracolor one Cytotoxicity against human A549 cells after 96 hrs using tetracolor one	[PMID: 24900316]
HCT-116	IC₅₀	0.054 μM Compound: 7	Cytotoxicity against human HCT116 cells after 96 hrs using tetracolor one Cytotoxicity against human HCT116 cells after 96 hrs using tetracolor one	[PMID: 24900316]
HT-29	IC₅₀	0.128 μM Compound: 7	Cytotoxicity against human HT-29 cells after 96 hrs using tetracolor one Cytotoxicity against human HT-29 cells after 96 hrs using tetracolor one	[PMID: 24900316]

In Vitro

4-Formylcolchicine (compound 7) (0-5 μM; 24 h) shows antiproliferative activity with IC₅₀s of 1.007, 0.128, 0.054 μM for A549, HT-29, HCT116 cells, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

A549, HT-29, HCT116 cells
Concentration:

0-5 µM
Incubation Time:

24 h
Result:

Inhibited cell growth with IC₅₀s of 1.007, 0.128, 0.054 µM for A549, HT-29, HCT116 cells, respectively.

Chemical Information

CAS. Nr. 2730-82-7
Appearance Solid
Molecular Weight 427.45
Formel C₂₃H₂₅NO₇
Color Off-white to yellow
SMILES

COC1=C2C(C([C@H](CCC2=C(C(OC)=C1OC)C=O)NC(C)=O)=C3)=CC=C(OC)C3=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (233.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3395 mL

11.6973 mL

23.3945 mL

5 mM

0.4679 mL

2.3395 mL

4.6789 mL

10 mM

0.2339 mL

1.1697 mL

2.3395 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Formylcolchicine bound to lactosaminated serum albumin is a more active antifibrotic agent than free colchicine

[2]. Yasobu N, et al. Design, Synthesis, and Antitumor Activity of 4-Halocolchicines and Their Pro-drugs Activated by Cathepsin B. ACS Med Chem Lett. 2011 Mar 4;2(5):348-52.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3395 mL	11.6973 mL	23.3945 mL	58.4864 mL
	5 mM	0.4679 mL	2.3395 mL	4.6789 mL	11.6973 mL
	10 mM	0.2339 mL	1.1697 mL	2.3395 mL	5.8486 mL
	15 mM	0.1560 mL	0.7798 mL	1.5596 mL	3.8991 mL
	20 mM	0.1170 mL	0.5849 mL	1.1697 mL	2.9243 mL
	25 mM	0.0936 mL	0.4679 mL	0.9358 mL	2.3395 mL
	30 mM	0.0780 mL	0.3899 mL	0.7798 mL	1.9495 mL
	40 mM	0.0585 mL	0.2924 mL	0.5849 mL	1.4622 mL
	50 mM	0.0468 mL	0.2339 mL	0.4679 mL	1.1697 mL
	60 mM	0.0390 mL	0.1950 mL	0.3899 mL	0.9748 mL
	80 mM	0.0292 mL	0.1462 mL	0.2924 mL	0.7311 mL
	100 mM	0.0234 mL	0.1170 mL	0.2339 mL	0.5849 mL

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

4-Formylcolchicine