Art. -Nr.: HY-141425

NSC73306

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NSC73306, a thiosemicarbazone, is a cell penetrant, cytotoxic agent that exhibits greater toxicity against cells expressing functional P-gp (P-glycoprotein) than against other cells.

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Reinheit: 98.52%
CAS. Nr.: 79560-74-0
Formel: C₁₆H₁₄N₄O₂S
Molecular Weight:326.37

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

NSC73306 Chemical Structure

CAS. Nr.: 79560-74-0

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Biologische Aktivität
- Beschreibung
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

NSC73306, a thiosemicarbazone, is a cell penetrant, cytotoxic agent that exhibits greater toxicity against cells expressing functional P-gp (P-glycoprotein) than against other cells^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	7.49 μM Compound: 1a; NSC73306	Cytotoxicity against human A2780 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Cytotoxicity against human A2780 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 27161177]
A2780 ADR	IC₅₀	4.84 μM Compound: 1a; NSC73306	Cytotoxicity against human A2780adr cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Cytotoxicity against human A2780adr cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 27161177]
CHO	IC₅₀	0.97 μM Compound: 1, NSC73306	Cytotoxicity against CHO cells selected at 1 ug/mL colchicine after 72 hrs by MTT assay Cytotoxicity against CHO cells selected at 1 ug/mL colchicine after 72 hrs by MTT assay	[PMID: 21721528]
CHO	IC₅₀	3.04 μM Compound: 1, NSC73306	Cytotoxicity against CHO cells after 72 hrs by MTT assay Cytotoxicity against CHO cells after 72 hrs by MTT assay	[PMID: 21721528]
HEK293	IC₅₀	2.55 μM Compound: 1, NSC73306	Cytotoxicity against human HEK293 cells transfected with plasmid expressing MDR1 after 72 hrs by MTT assay Cytotoxicity against human HEK293 cells transfected with plasmid expressing MDR1 after 72 hrs by MTT assay	[PMID: 21721528]
HEK293	IC₅₀	6.23 μM Compound: 1, NSC73306	Cytotoxicity against human HEK293 cells after 72 hrs by MTT assay Cytotoxicity against human HEK293 cells after 72 hrs by MTT assay	[PMID: 21721528]
KB 3-1	IC₅₀	11.37 μM Compound: 1, NSC73306	Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 10 uM CsA Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 10 uM CsA	[PMID: 21721528]
KB 3-1	IC₅₀	12.09 μM Compound: 1, NSC73306	Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 100 uM TQR Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 100 uM TQR	[PMID: 21721528]
KB 3-1	IC₅₀	12.41 μM Compound: 1, NSC73306	Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 100 uM DCPQ Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 100 uM DCPQ	[PMID: 21721528]
KB 3-1	IC₅₀	14.2 μM Compound: 1, NSC73306	Cytotoxicity against human KB-3-1 cells after 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells after 72 hrs by MTT assay	[PMID: 21721528]
KB 3-1	IC₅₀	14.2 μM Compound: 1, NSC-73306	Cytotoxicity against human P-gp-negative KB-3-1 cells after 72 hrs by MTT assay Cytotoxicity against human P-gp-negative KB-3-1 cells after 72 hrs by MTT assay	[PMID: 19397322]
KB 3-1	IC₅₀	14.2 μM Compound: 44a	Cytotoxicity against human KB-3-1 cells measured after 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells measured after 72 hrs by MTT assay	[PMID: 27391135]
KB 3-1	IC₅₀	7.82 μM Compound: 1a; NSC73306	Cytotoxicity against human KB-3-1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 27161177]
KB 3-1	IC₅₀	9.68 μM Compound: 1, NSC73306	Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 1 uM verapamil Cytotoxicity against P-gp-negative human KB-3-1 cells after 72 hrs by MTT assay in presence of 1 uM verapamil	[PMID: 21721528]
KB-V1	IC₅₀	11.55 μM Compound: 1, NSC73306	Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 100 uM TQR Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 100 uM TQR	[PMID: 21721528]
KB-V1	IC₅₀	14.03 μM Compound: 1, NSC73306	Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 100 uM DCPQ Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 100 uM DCPQ	[PMID: 21721528]
KB-V1	IC₅₀	2.23 μM Compound: 1, NSC73306	Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 1 uM verapamil Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 1 uM verapamil	[PMID: 21721528]
KB-V1	IC₅₀	3.3 μM Compound: 1, NSC73306	Cytotoxicity against vinblastine-selected human KBV1 cells after 72 hrs by MTT assay Cytotoxicity against vinblastine-selected human KBV1 cells after 72 hrs by MTT assay	[PMID: 21721528]
KB-V1	IC₅₀	3.3 μM Compound: 1, NSC-73306	Cytotoxicity against human P-glycoprotein-expressing KBV1 cells after 72 hrs by MTT assay Cytotoxicity against human P-glycoprotein-expressing KBV1 cells after 72 hrs by MTT assay	[PMID: 19397322]
KB-V1	IC₅₀	3.3 μM Compound: 44a	Cytotoxicity against human KBV1 cells expressing P-glycoprotein measured after 72 hrs by MTT assay Cytotoxicity against human KBV1 cells expressing P-glycoprotein measured after 72 hrs by MTT assay	[PMID: 27391135]
KB-V1	IC₅₀	3.78 μM Compound: 1a; NSC73306	Cytotoxicity against human KB-V1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Cytotoxicity against human KB-V1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 27161177]
KB-V1	IC₅₀	8.33 μM Compound: 1, NSC73306	Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 10 uM CsA Cytotoxicity against P-gp expressing human KBV1 cells after 72 hrs by MTT assay in presence of 10 uM CsA	[PMID: 21721528]
LLC-PK1	IC₅₀	3.99 μM Compound: 1, NSC73306	Cytotoxicity against pig LLC-PK1 cells after 72 hrs by MTT assay Cytotoxicity against pig LLC-PK1 cells after 72 hrs by MTT assay	[PMID: 21721528]
LLC-PK1	IC₅₀	36.79 μM Compound: 1, NSC73306	Cytotoxicity against pig LLC-PK1 cells transfected with empty vector after 72 hrs by MTT assay Cytotoxicity against pig LLC-PK1 cells transfected with empty vector after 72 hrs by MTT assay	[PMID: 21721528]
MDA-MB-231	IC₅₀	13.82 μM Compound: 1, NSC73306	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 21721528]
MDA-MB-231	IC₅₀	4.86 μM Compound: 1, NSC73306	Cytotoxicity against human MDA-MB-231 cells selected at 100 ug/ml vinblastine after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells selected at 100 ug/ml vinblastine after 72 hrs by MTT assay	[PMID: 21721528]
MES-SA	IC₅₀	12.06 μM Compound: 1a; NSC73306	Cytotoxicity against human MES-SA cells transfected with mCherry fluorescent protein assessed as inhibition of cell proliferation incubated for 72 hrs by fluorescent assay Cytotoxicity against human MES-SA cells transfected with mCherry fluorescent protein assessed as inhibition of cell proliferation incubated for 72 hrs by fluorescent assay	[PMID: 27161177]
MES-SA	IC₅₀	13.77 μM Compound: 1, NSC73306	Cytotoxicity against adriamycin-selected human MES-SA cells after 72 hrs by MTT assay Cytotoxicity against adriamycin-selected human MES-SA cells after 72 hrs by MTT assay	[PMID: 21721528]
MES-SA	IC₅₀	22.4 μM Compound: 1, NSC73306	Cytotoxicity against human MES-SA cells after 72 hrs by MTT assay Cytotoxicity against human MES-SA cells after 72 hrs by MTT assay	[PMID: 21721528]
MES-SA	IC₅₀	6.1 μM Compound: 1a; NSC73306	Cytotoxicity against human MES-SA cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Cytotoxicity against human MES-SA cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 27161177]
MES-SA/Dx5	IC₅₀	3.59 μM Compound: 1a; NSC73306	Cytotoxicity against human MES-SA/DX5 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Cytotoxicity against human MES-SA/DX5 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 27161177]
MES-SA/Dx5	IC₅₀	4.15 μM Compound: 1a; NSC73306	Cytotoxicity against human MES-SA/DX5 cells transfected with mCherry fluorescent protein assessed as inhibition of cell proliferation incubated for 72 hrs by fluorescent assay Cytotoxicity against human MES-SA/DX5 cells transfected with mCherry fluorescent protein assessed as inhibition of cell proliferation incubated for 72 hrs by fluorescent assay	[PMID: 27161177]
NCI/ADR-RES	IC₅₀	3.79 μM Compound: 1, NSC73306	Cytotoxicity against adriamycin-selected human NCI/ADR-RES cells after 72 hrs by MTT assay Cytotoxicity against adriamycin-selected human NCI/ADR-RES cells after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	1.06 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells transfected with wild-type P-gp G185 after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells transfected with wild-type P-gp G185 after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	1.97 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells transfected with PO4 mutant harboring Ser to Asp mutation selected at 2400 ug/ml vincristine after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells transfected with PO4 mutant harboring Ser to Asp mutation selected at 2400 ug/ml vincristine after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	2.23 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells transfected with PO4 mutant harboring four Ser to Arg mutation selected at 2400 ug/ml vincristine after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells transfected with PO4 mutant harboring four Ser to Arg mutation selected at 2400 ug/ml vincristine after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	3.14 μM Compound: 1, NSC73306	Cytotoxicity against 2.4 ug/mL vincristine selected mouse NIH/3T3 cells transfected with P-gp Ser to Asp phosphorylation mutant after 72 hrs by MTT assay Cytotoxicity against 2.4 ug/mL vincristine selected mouse NIH/3T3 cells transfected with P-gp Ser to Asp phosphorylation mutant after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	3.17 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells selected at 1 ug/mL colchicine after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells selected at 1 ug/mL colchicine after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	3.59 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells transfected with wild type p-glycoprotein ABCB1 selected at 600 ug/ml vincristine after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells transfected with wild type p-glycoprotein ABCB1 selected at 600 ug/ml vincristine after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	4.58 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells selected at 30 ug/ml vincristine after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells selected at 30 ug/ml vincristine after 72 hrs by MTT assay	[PMID: 21721528]
NIH3T3	IC₅₀	8.12 μM Compound: 1, NSC73306	Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay	[PMID: 21721528]
OVCAR-8	IC₅₀	6.69 μM Compound: 1, NSC73306	Cytotoxicity against human OVCAR8 cells after 72 hrs by MTT assay Cytotoxicity against human OVCAR8 cells after 72 hrs by MTT assay	[PMID: 21721528]
RD	CC₅₀	>25 μM Compound: 16	Cytotoxicity against human RD cells Cytotoxicity against human RD cells	[PMID: 21356589]
SW-620	IC₅₀	3.74 μM Compound: 1, NSC73306	Cytotoxicity against human SW620 cells selected at 20 ng/mL adriamycin after 72 hrs by MTT assay Cytotoxicity against human SW620 cells selected at 20 ng/mL adriamycin after 72 hrs by MTT assay	[PMID: 21721528]
SW-620	IC₅₀	9.04 μM Compound: 1, NSC73306	Cytotoxicity against human SW620 cells after 72 hrs by MTT assay Cytotoxicity against human SW620 cells after 72 hrs by MTT assay	[PMID: 21721528]

Chemical Information

CAS. Nr. 79560-74-0
Appearance Solid
Molecular Weight 326.37
Formel C₁₆H₁₄N₄O₂S
Color Yellow to orange
SMILES

O=C1NC2=C(C=CC=C2)/C1=N/NC(NC3=CC=C(OC)C=C3)=S
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 100 mg/mL (306.40 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0640 mL

15.3200 mL

30.6401 mL

5 mM

0.6128 mL

3.0640 mL

6.1280 mL

10 mM

0.3064 mL

1.5320 mL

3.0640 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ludwig JA, et al. Selective toxicity of NSC73306 in MDR1-positive cells as a new strategy to circumvent multidrug resistance in cancer. Cancer Res. 2006;66(9):4808-4815. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0640 mL	15.3200 mL	30.6401 mL	76.6002 mL
	5 mM	0.6128 mL	3.0640 mL	6.1280 mL	15.3200 mL
	10 mM	0.3064 mL	1.5320 mL	3.0640 mL	7.6600 mL
	15 mM	0.2043 mL	1.0213 mL	2.0427 mL	5.1067 mL
	20 mM	0.1532 mL	0.7660 mL	1.5320 mL	3.8300 mL
	25 mM	0.1226 mL	0.6128 mL	1.2256 mL	3.0640 mL
	30 mM	0.1021 mL	0.5107 mL	1.0213 mL	2.5533 mL
	40 mM	0.0766 mL	0.3830 mL	0.7660 mL	1.9150 mL
	50 mM	0.0613 mL	0.3064 mL	0.6128 mL	1.5320 mL
	60 mM	0.0511 mL	0.2553 mL	0.5107 mL	1.2767 mL
	80 mM	0.0383 mL	0.1915 mL	0.3830 mL	0.9575 mL
	100 mM	0.0306 mL	0.1532 mL	0.3064 mL	0.7660 mL

Keywords

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NSC73306

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

NSC73306 Related Classifications

HY-141425 Related Classifications