Creatinase, Actinobacteria
Based on 1 Customer Validation
Creatinase, Actinobacteria (Creatine amidohydrolase) is a hydrolase that catalyzes the hydrolysis of creatine into sarcosine and urea, and plays an important role in the enzymatic determination of creatinine concentration.
For research use only. We do not sell to patients.
- CAS No.: 37340-58-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
Product Information
This product can hydrolyze creatine and is used in the development and preparation of enzymatic creatinine reagents.
Molecular weight: 48 kDa (SDS-PAGE)
Isoelectric point: 4.6
Inhibitors: Ag +, Cu 2+, Hg 2+
Optimum: pH 7.5
Optimum temperature: 45-50°C
pH stability 4.5-10.0 (25°C, 16 h)
Instructions
Can be dissolved in water (1 mg/mL), or according to specific experimental references (such as using 50 mM PBS, pH 7.5).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
EC Number
3.5.3.3
Enzyme Activity
≥10 U/mg soild
Unit Definition
One unit is defined as the amount of enzyme that can react creatine to produce 1 μmol sarcosine per minute at 37°C and pH 5.5.
Chemical Information
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CAS No. 37340-58-2
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Appearance Solid
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Color White to off-white
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SMILES
[Creatinase, Actinobacteria]
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Synonyms
Creatine amidohydrolase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (Need ultrasonic)
Protocols
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Nephrotoxicity Study
This protocol assesses nephrotoxicity by combining functional kidney injury readouts, urinary/tissue injury biomarkers, and renal histopathology. Serum creatinine and BUN reflect impaired kidney function, while KIM-1, NGAL, clusterin, osteopontin, IL-18, cystatin C, nephrin, Oat5, urinary protein, glucose, and alkaline phosphatase have been used to detect tubular injury in cisplatin-, gentamicin-, and acetaminophen-induced nephrotoxicity models.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Creatinase, Actinobacteria Related Classifications
HY-P2893 Related Classifications
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