Creatininase, Microorganism
Based on 2 publication(s) in Google Scholar
Creatininase, Microorganism (Creatinine amidohydrolase; CAH), namely creatinine amidohydrolase, from Pseudomonas putida, is a homohexameric enzyme commonly used in biochemical research. Creatininase acts on carbon-nitrogen bonds other than peptide bonds, and can catalyze the hydrolysis of creatinine to creatine, which can then be metabolized by creatinase to urea and sarcosine.
For research use only. We do not sell to patients.
- CAS No.: 9025-13-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Creatininase, Microorganism
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Product Information
Molecular weight: 29 kDa (SDS-PAGE)
Isoelectric point: 5.3
Km value: 5.0×10-2 M (Creatinine), 8.0×10-2 M (Creatine)
Inhibitors: Hg2+, Cu2+, Fe3+
Optimum pH: 7.0-8.0
Optimum temperature: 65°C
pH stability: pH 5.5-10.0 (25°C, 16 h)
Thermal stability: stable below 65°C (pH 8.0, 30 min)
Instructions
The enzyme can be dissolved in pure water (below 50 mg/mL concentration), or in buffer according to the specific experimental references.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.5.2.10
≥400 U/mg
Chemical Information
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CAS No. 9025-13-2
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Appearance Solid
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Color White to off-white
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SMILES
[Creatininase, Microorganism]
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Synonyms
Creatinine amidohydrolase; CAH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
H2O : 50 mg/mL (Need ultrasonic)
Purity & Documentation
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Data Sheet (268 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)