Demethylsuberosin
Based on 4 publication(s) in Google Scholar
Demethylsuberosin (7-Demethylsuberosin) is a coumarin compound found in Angelica gigas Nakai. Demethylsuberosin exerts antihypertensive effects by inhibiting the L-type CaV1.2 channel. Demethylsuberosin has antioxidant and anti-inflammatory activities. Demethylsuberosin also exhibits neuroprotective activities against glutamate-induced toxicity in primary cultured rat cortical cells.
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- Pureza : 99.71%
- No. CAS: 21422-04-8
- Fòrmula: C14H14O3
- Peso molecular:230.26
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
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Publications Citing Use of MedChemExpress (MCE) Demethylsuberosin
MoreVer todos los productos específicos de isoformas Calcium Channel
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| SH-SY5Y | EC50 |
0.2 μM
Compound: 31
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Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay
Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay
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[PMID: 27420919] |
| SH-SY5Y | EC50 |
8.6 μM
Compound: 31
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Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay
Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay
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[PMID: 27420919] |
| Vero | IC50 |
>50 μg/mL
Compound: 6
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Cytotoxicity against african green monkey Vero cells
Cytotoxicity against african green monkey Vero cells
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[PMID: 21985060] |
In Vitro
Demethylsuberosin (7-Demethylsuberosine shows good ABTS+ radical scavenging activity (IC50 of 8.1 μg/mL)[1].
Demethylsuberosin significantly suppresses NO production in Lipopolysaccharides (HY-D1056) (LPS)-stimulated RAW 264.7 cells, with an IC50 of 7.6 μg/mL. Demethylsuberosin effectively suppresses the expression levels of iNOS, IL-1β, and COX-2, which are responsible for promoting the inflammatory process[1].
Demethylsuberosin (100 nM-50 μM) shows a dose-dependent inhibition of CaV1.2 channel currents in HEK293T cells expressing the CaV1.2 channel, with an IC50 of 3.23 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice (both sexes, 20-25 g, 6-8 weeks old) induced by 300 mg/L L-NAME in drinking water for four weeks[2]
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Dosage:1 mg/kg, 5 mg/kg, 50 mg/kg
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Administration:Intragastric administration, once a day, for 28 days
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Result:Significantly reduced blood pressure compared to the L-NAME group. It also reduced blood pressure in a dose-dependent manner.
Chemical Information
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No. CAS 21422-04-8
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Appearance Solid
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Peso molecular 230.26
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Fòrmula C14H14O3
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Color White to off-white
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SMILES
O=C1C=CC2=CC(C/C=C(C)\C)=C(O)C=C2O1
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Synonyms
7-Demethylsuberosin
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Structure Classification
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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Pharmacol Res
Glycyrol exerts potent therapeutic effect on lung cancer via directly inactivating T-LAK cell-originated protein kinase. [Abstract]2019 Sep:147:104366. PMID: 31377221 -
Int J Mol Sci
2024 Feb 20;25(5):2458. PMID: 38473706 -
Food Chem Toxicol
Synergistic anti-colon cancer effect of glycyrol and butyrate is associated with the enhanced activation of caspase-3 and structural features of glycyrol. [Abstract]2020 Feb:136:110952. PMID: 31712101 -
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (434.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[2]. Yang X, et al. Angelica decursiva exerts antihypertensive activity by inhibiting L-type calcium channel. J Ethnopharmacol. 2023 Sep 15;313:116527. [Content Brief]
[3]. Kang SY, et al. Neuroprotective coumarins from the root of Angelica gigas: structure-activity relationships. Arch Pharm Res. 2007 Nov;30(11):1368-73. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3429 mL | 21.7146 mL | 43.4292 mL | 108.5729 mL |
| 5 mM | 0.8686 mL | 4.3429 mL | 8.6858 mL | 21.7146 mL | |
| 10 mM | 0.4343 mL | 2.1715 mL | 4.3429 mL | 10.8573 mL | |
| 15 mM | 0.2895 mL | 1.4476 mL | 2.8953 mL | 7.2382 mL | |
| 20 mM | 0.2171 mL | 1.0857 mL | 2.1715 mL | 5.4286 mL | |
| 25 mM | 0.1737 mL | 0.8686 mL | 1.7372 mL | 4.3429 mL | |
| 30 mM | 0.1448 mL | 0.7238 mL | 1.4476 mL | 3.6191 mL | |
| 40 mM | 0.1086 mL | 0.5429 mL | 1.0857 mL | 2.7143 mL | |
| 50 mM | 0.0869 mL | 0.4343 mL | 0.8686 mL | 2.1715 mL | |
| 60 mM | 0.0724 mL | 0.3619 mL | 0.7238 mL | 1.8095 mL | |
| 80 mM | 0.0543 mL | 0.2714 mL | 0.5429 mL | 1.3572 mL | |
| 100 mM | 0.0434 mL | 0.2171 mL | 0.4343 mL | 1.0857 mL |
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