Dimethyl malonate

Name: Dimethyl malonate
Brand: MedChemExpress
SKU: HY-Y1787
Rating: 5.0 (9 reviews)

Cat. No.: HY-Y1787 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Dimethyl malonate is a competitive inhibitor of succinate dehydrogenase (SDH). Dimethyl malonate is able to cross the blood-brain barrier and hydrolyse to malonate. Dimethyl malonate reduces neuronal apoptosis.

For research use only. We do not sell to patients.

CAS No. : 108-59-8

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Dimethyl malonate:

Dimethyl malonate (Standard) In-stock

9 Publications Citing Use of MCE Dimethyl malonate

Flow Cytometry

Bio/Physico-chemical Assay

In Vivo Efficacy Study

Histological Imaging/Staining

: Dimethyl malonate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 28;16(1):10751. [Abstract]; Flow cytometry detected mitochondrial ROS levels (MitoSox Red) in HepG2 cells. The HepG2 cells are transfected with NC or si-NEK7 for 48 h, then treated with 500 μM Tempo, 10 μM Dimethyl malonate (DMM), 100 μM Metformin (Met), or 50 nM Rotenone (Rot) for 24 h, respectively. N = 3/group. The results showed that DMM significantly attenuated the increase in ROS levels induced by NEK7 deletion in HepG2 cells.

: Dimethyl malonate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 28;16(1):10751. [Abstract]; Representative TEM images depicting the ultrastructure of the mitochondria in hepatocytes from the Het+CCl4 and Het+CCl4+DMM mice (scale bar, 5μm). N=3/group. The results showed that Dimethyl malonate (DMM, 200 mg/kg; oral gavage 2-3 h after every CCl₄-treatment) (targeting complex Ⅱ) could rescue hepatic mitochondrial damage induced by NEK7 knockdown, suggesting that mitochondrial structural disorganization may result from impaired complex Ⅱ activity.

: Dimethyl malonate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 28;16(1):10751. [Abstract]; Western blotting analysis of the expression of α-SMA, Collagen Ⅰ, and Fibronectin protein in the LX-2 cells treated with the conditional medium of HepG2 cells. The HepG2 cells are transfected with NC or si-NEK7 for 48 h, then treated with 500 μM Tempo and 10 μM Dimethyl malonate (DMM) for 24 h, respectively.

: Dimethyl malonate purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 Apr 18:S1534-5807(25)00206-0. [Abstract]; Measurement of succinate dehydrogenase complex (SDH) activity in NCI-H1299 cellstreated with either DMSO or Dimethyl malonate (DMM; 10 mM) for 3 h. The results showed that Dimethyl malonate (DMM) significantly inhibited the activity of SDH.

: Dimethyl malonate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Jul 23;16(1):399. [Abstract]; Dimethyl malonate (DMM) (100 mg/kg; s.c.) significantly reduced the residual wound area in diabetic mice on day 14 (D14), and further decreased the wound area on day 21 (D21). At this stage, only slight scab formation was observed in the control group (Ctrl group). No scar contracture was detected in any group; the regenerated epidermis exhibited a healthy morphology, and no skin contraction was observed.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vivo

Dimethyl malonate (6 mg/kg/min; intravenous infusion; 51 min) promotes return of spontaneous circulation (ROSC) and neurological performance in rats after cardiac arrest^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley male rats, cardiac arrest model^[1]
Dosage:	6 mg/kg/min
Administration:	Intravenous infusion, 51 min
Result:	Improved ROSC after CA. Prevented the decline in neurological function and inhibited the apoptosis of hippocampal neurons at day 3 after CPR. Inhibited caspase-3 cleavage and increased HIF-1α expression at day 3 after CPR. Decreased the level of oxidative stress at day 3 after CPR. Inhibited excessive hyperpolarization of MMP and restrained the leakage of cytochrome C after 45 min reperfusion.

Clinical Trial

Molecular Weight

132.11

Formula

C₅H₈O₄

CAS No.

108-59-8

Appearance

Liquid (Density: 1.156 g/cm³)

Color

Colorless to light yellow

SMILES

O=C(OC)CC(OC)=O

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (756.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (756.94 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.5694 mL	37.8472 mL	75.6945 mL
5 mM	1.5139 mL	7.5694 mL	15.1389 mL
10 mM	0.7569 mL	3.7847 mL	7.5694 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (18.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (244 KB)

Handling Instructions (2659 KB)

References

[1]. Xu J, et al. Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest. Neuroscience. 2018 Nov 21;393:24-32. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	7.5694 mL	37.8472 mL	75.6945 mL	189.2362 mL
	5 mM	1.5139 mL	7.5694 mL	15.1389 mL	37.8472 mL
	10 mM	0.7569 mL	3.7847 mL	7.5694 mL	18.9236 mL
	15 mM	0.5046 mL	2.5231 mL	5.0463 mL	12.6157 mL
	20 mM	0.3785 mL	1.8924 mL	3.7847 mL	9.4618 mL
	25 mM	0.3028 mL	1.5139 mL	3.0278 mL	7.5694 mL
	30 mM	0.2523 mL	1.2616 mL	2.5231 mL	6.3079 mL
	40 mM	0.1892 mL	0.9462 mL	1.8924 mL	4.7309 mL
	50 mM	0.1514 mL	0.7569 mL	1.5139 mL	3.7847 mL
	60 mM	0.1262 mL	0.6308 mL	1.2616 mL	3.1539 mL
	80 mM	0.0946 mL	0.4731 mL	0.9462 mL	2.3655 mL
	100 mM	0.0757 mL	0.3785 mL	0.7569 mL	1.8924 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.