Coelenterazine TFA
Based on 6 publication(s) in Google Scholar
Coelenterazine TFA is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate Coelenterazine TFA has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine TFA is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite (Ex/Em = 429/466 nm).
For research use only. We do not sell to patients.
- Purity: 99.14%
- Formula: C28H22F3N3O5
- Molecular Weight:537.49
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Coelenterazine TFA
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Biological Activity
HCT-8 control cells, transiently expressing Renilla luciferase (RLuc), showed low bioluminescence due to P-glycoprotein–mediated efflux transport of Coelenterazine TFA. By comparison, transiently expressing RLuc HCT-8 cells, wherein P-glycoprotein was down-regulated with shRNAi, showed high bioluminescence[3].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of free radical production[5].
1. The measurements are performed in 0.1 M potassium phosphate buffer, pH 7.0.
2. Coelenterazine TFA (2.5 μM) is added to the buffer immediately prior
to measurements.
3. Add 2 mg/mL NADH.
4. At room temperature using a luminometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Coelenterazine (2 mg/kg) TFA is injected intravenously.
After 5 minutes, the mice are fixed and imaged for 3 to 5 minutes.
All mice demonstrated very high Rluc activity at the primary site with the majority of mice simultaneously showing metastases to inguinal ILNs[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 537.49
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Formula C28H22F3N3O5
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Color Light yellow to yellow
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SMILES
O=C1C(CC2=CC=C(O)C=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=C(O)C=C5)=CN31.FC(F)(C(O)=O)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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ACS Sens
Secretory Bioluminescent Reporter Sensor for Functional Assessment of Glucocorticoids In Vitro and In Vivo. [Abstract]2026 Jun 26. PMID: 42358115 -
Plant Cell Environ
A Rapid and Reversible Molecular "Switch" Regulating Protein Expression in Chlamydomonas reinhardtii. [Abstract]2025 Jun;48(6):3913-3924. PMID: 39838873 -
Prostate Cancer Prostatic Dis
Effects of dietary omega-3 fatty acids on orthotopic prostate cancer progression, tumor associated macrophages, angiogenesis and T-cell activation-dependence on GPR120. [Abstract]2022 Sep;25(3):539-546. PMID: 35075215 -
FASEB J
2025 Jun 15;39(11):e70624. PMID: 40444459 -
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Solvent & Solubility
DMSO : 100 mg/mL (186.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (291 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Markova SV, et al, Coelenterazine-dependent luciferases. Biochemistry (Mosc). 2015 Jun;80(6):714-32. [Content Brief]
[2]. Lucas M, et al. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils. Anal Biochem. 1992 Nov 1;206(2):273-7. [Content Brief]
[3]. Pichler A, et al. In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein. Clin Cancer Res. 2005 Jun 15;11(12):4487-94. [Content Brief]
[4]. Volk-Draper LD, et al. Novel model for basaloid triple-negative breast cancer: behavior in vivo and response to therapy. Neoplasia. 2012 Oct;14(10):926-42. [Content Brief]
[5]. Raha S, et al. Control of oxygen free radical formation from mitochondrial complex I: roles for protein kinase A and pyruvate dehydrogenase kinase. Free Radic Biol Med. 2002 Mar 1;32(5):421-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8605 mL | 9.3025 mL | 18.6050 mL | 46.5125 mL |
| 5 mM | 0.3721 mL | 1.8605 mL | 3.7210 mL | 9.3025 mL | |
| 10 mM | 0.1860 mL | 0.9302 mL | 1.8605 mL | 4.6512 mL | |
| 15 mM | 0.1240 mL | 0.6202 mL | 1.2403 mL | 3.1008 mL | |
| 20 mM | 0.0930 mL | 0.4651 mL | 0.9302 mL | 2.3256 mL | |
| 25 mM | 0.0744 mL | 0.3721 mL | 0.7442 mL | 1.8605 mL | |
| 30 mM | 0.0620 mL | 0.3101 mL | 0.6202 mL | 1.5504 mL | |
| 40 mM | 0.0465 mL | 0.2326 mL | 0.4651 mL | 1.1628 mL | |
| 50 mM | 0.0372 mL | 0.1860 mL | 0.3721 mL | 0.9302 mL | |
| 60 mM | 0.0310 mL | 0.1550 mL | 0.3101 mL | 0.7752 mL | |
| 80 mM | 0.0233 mL | 0.1163 mL | 0.2326 mL | 0.5814 mL | |
| 100 mM | 0.0186 mL | 0.0930 mL | 0.1860 mL | 0.4651 mL |
Coelenterazine TFA Related Classifications
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