1-Methylinosine
Based on 3 publication(s) in Google Scholar
1-Methylinosine (N1-MetHYlinosine) is a modified nucleotide located at position 37 of eukaryotic tRNA, 3' to the tRNA anticodon. 1-Methylinosine is a minor metabolite of 1-methyladenosine (HY-113081). The level of 1-Methylinosine is significantly elevated in urine samples from breast cancer models.
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- Pureza: 99.72%
- No. CAS: 2140-73-0
- Fòrmula: C11H14N4O5
- Peso molecular:282.25
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-113137
N2,N2-Dimethylguanosine
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HY-W011793
4-Thiouridine
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HY-113136
1-Methylguanosine
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HY-122524
7-Methylguanosine
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HY-113081
1-Methyladenosine
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HY-W011209
N6-Isopentenyladenosine
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HY-N0092
Inosine
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HY-N0097
Guanosine
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HY-113216
Asymmetric dimethylarginine
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HY-101410
SDMA
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HY-N0097R
Guanosine (Standard)
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HY-111645
3-Methylcytidine
Publications Citing Use of MedChemExpress (MCE) 1-Methylinosine
MoreVer todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
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Human Endogenous Metabolite |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2140-73-0
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Appearance Solid
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Peso molecular 282.25
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Fòrmula C11H14N4O5
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Color White to off-white
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SMILES
OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=CN(C)C3=O)=C3N=C2)O1
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Synonyms
N1-Methylinosine
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Chem Biol
MapID-based quantitative mapping of chemical modifications and expression of human transfer RNA. [Abstract]2025 May 15;32(5):752-766.e7. PMID: 40318625 -
Talanta
High-throughput profiling of RNA modifications by ultra-performance liquid chromatography coupled to complementary mass spectrometry: Methods, quality control, and applications. [Abstract]2023 Oct 1:263:124697. PMID: 37262985 -
Solvente y solubilidad
DMSO : 83.33 mg/mL (295.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5430 mL | 17.7148 mL | 35.4296 mL | 88.5740 mL |
| 5 mM | 0.7086 mL | 3.5430 mL | 7.0859 mL | 17.7148 mL | |
| 10 mM | 0.3543 mL | 1.7715 mL | 3.5430 mL | 8.8574 mL | |
| 15 mM | 0.2362 mL | 1.1810 mL | 2.3620 mL | 5.9049 mL | |
| 20 mM | 0.1771 mL | 0.8857 mL | 1.7715 mL | 4.4287 mL | |
| 25 mM | 0.1417 mL | 0.7086 mL | 1.4172 mL | 3.5430 mL | |
| 30 mM | 0.1181 mL | 0.5905 mL | 1.1810 mL | 2.9525 mL | |
| 40 mM | 0.0886 mL | 0.4429 mL | 0.8857 mL | 2.2143 mL | |
| 50 mM | 0.0709 mL | 0.3543 mL | 0.7086 mL | 1.7715 mL | |
| 60 mM | 0.0590 mL | 0.2952 mL | 0.5905 mL | 1.4762 mL | |
| 80 mM | 0.0443 mL | 0.2214 mL | 0.4429 mL | 1.1072 mL | |
| 100 mM | 0.0354 mL | 0.1771 mL | 0.3543 mL | 0.8857 mL |
1-Methylinosine Related Classifications
HY-113139 Related Classifications
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