NCF2 - neutrophil cytosolic factor 2 Gene

Also Known as NCF-2; NOXA2; P67PHOX; P67-PHOX

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 4688

About NCF2

Cytogenetic location: 1q25.3 Genomic coordinates (GRCh38): 1:183,555,562-183,601,849 (from NCBI)

This gene has 12 transcripts (splice variants), 203 orthologues, 1 paralogue and is associated with 3 phenotypes. Broad expression in bone marrow (RPKM 41.9), appendix (RPKM 36.2) and 15 other tissues.

Summary

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH Oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH Oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

NCF2 Products (5)

mRNA	Protein	Name
NM_000433.4	NP_000424.2	neutrophil cytosol factor 2 isoform 1
NM_001127651.3	NP_001121123.1	neutrophil cytosol factor 2 isoform 1
NM_001190789.2	NP_001177718.1	neutrophil cytosol factor 2 isoform 3
NM_001190794.2	NP_001177723.1	neutrophil cytosol factor 2 isoform 2
NM_001410895.1	NP_001397824.1	neutrophil cytosol factor 2 isoform 4

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables protein binding	IPI IPI: Inferred from physical interaction	7938008	GOA
enables superoxide-generating NADPH oxidase activator activity	IMP IMP: Inferred from mutant phenotype	8280052	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in superoxide anion generation	IMP IMP: Inferred from mutant phenotype	8280052	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of NADPH oxidase complex	IDA IDA: Inferred from direct assay	8280052	GOA
located in cytosol	IDA IDA: Inferred from direct assay	8280052	GOA
located in membrane	IDA IDA: Inferred from direct assay	8280052	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

NCF2 Protein Structure

TPR_11

SH3_1

PB1

SH3_1

0
100
200
300
400
500
526 a.a.

Protein Preferred Names	Protein Names
neutrophil cytosol factor 2	67 kDa neutrophil oxidase factor

NCF2 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	NCF2	P19878	NCF4	Homo sapiens	Q15080	Anti Tag CoIP	16782902
Intra	NCF2	P19878	GNAI2	Homo sapiens	P04899	Anti Bait CoIP	16782902
Intra	NCF2	P19878	GNAI2	Homo sapiens	P04899	Anti Tag CoIP	16782902
Intra	NCF2	P19878	GNB1	Homo sapiens	P62873	Anti Tag CoIP	16782902
Intra	NCF2	P19878	NCF1	Homo sapiens	P14598	NMR	12169629
Intra	NCF2	P19878	NCF1	Homo sapiens	P14598	Pull Down	16297854
Intra	NCF2	P19878	NCF1	Homo sapiens	P14598	Fluorescence Spectr	15657040
Intra	NCF2	P19878	NCF1	Homo sapiens	P14598	Anti Bait CoIP	16297854
Intra	NCF2	P19878	NCF1	Homo sapiens	P14598	Anti Tag CoIP	16782902
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Validated Y2H	25910212
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Y2H Prey Pooling	25416956
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Y2H Prey Pooling	26871637
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Y2H Array	25910212
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Validated Y2H	26871637
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Y2H Array	25416956
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Y2H Array	26871637
Intra	NCF2	P19878	GOLGA2	Homo sapiens	Q08379	Y2H Bait-Prey Pool	25910212
Intra	NCF2	P19878	PRKCD	Homo sapiens	Q05655	Protein Kinase Assay	15591124
Intra	NCF2	P19878	PRKCD	Homo sapiens	Q05655	Anti Bait CoIP	15591124
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Bait-Prey Pool	25910212
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Array	32296183
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Validated Y2H	25416956
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Validated Y2H	26871637
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Validated Y2H	32296183
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Prey Pooling	26871637
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Prey Pooling	25416956
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Array	25910212
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Array	26871637
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Prey Pooling	32296183
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H Array	25416956
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Y2H	21516116
Intra	NCF2	P19878	KIFAP3	Homo sapiens	Q92845	Validated Y2H	25910212

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant NCF2 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P74655	p67phox Protein, Human (sf9, His-GST)	P19878 (M1-V526)	≥ 90%, as determined by reducing SDS-PAGE.
HY-P74656	p67phox Protein, Human (sf9)	P19878 (N-G&P, M1-V526)	≥ 85%, as determined by reducing SDS-PAGE.

NCF2 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P81904	NOXA2 Antibody (YA1649)	WB, IHC-P, ICC/IF, IP	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Granulomatous Disease, Chronic, Autosomal Recessive, 2	Chronic Granulomatous Disease Due To Deficiency Of Ncf-2 CGD2 Ncf2 Deficiency P67-Phox Deficiency Autosomal Recessive Chronic Granulomatous Disease Cytochrome B-Positive Type Ii Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-Positive, Type 2 Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-Positive, Type Ii Cgd, Autosomal Recessive Cytochrome B-Positive, Type Ii Granulomatous Disease, Chronic, Due To Ncf2 Deficiency Neutrophil Cytosol Factor 2 Deficiency Chronic Granulomatous Disease 2, Autosomal Recessive Autosomal Recessive Chronic Granulomatous Disease 2 Cdg2 Deficiency Of Ncf2 Deficiency Of P67-Phox Chronic Granulomatous Disease Autosomal Recessive Cytochrome B-Positive Type Ii Deficiency Of Neutrophil Cytosol Factor 2 Avellino Corneal Dystrophy
Chronic Granulomatous Disease	Cgd Granulomatous Disease, Chronic Autosomal Recessive Chronic Granulomatous Disease X-Linked Chronic Granulomatous Disease Bridges-Good Syndrome Congenital Dysphagocytosis Quie Syndrome Chronic Septic Granulomatosis Chronic Granulomatous Disorder Granulomatous Disease Chronic Granulomatous Disease, Chronic, X-Linked
Phagocyte Bactericidal Dysfunction	Phagocytic Dysfunction
Granulomatous Disease, Chronic, Autosomal Recessive, 1	Chronic Granulomatous Disease Due To Deficiency Of Ncf-1 CGD1 Ncf1 Deficiency Soluble Oxidase Component Ii Deficiency Soc2 Deficiency P47-Phox Deficiency Autosomal Recessive Chronic Granulomatous Disease Cytochrome B-Positive Type I Deficiency Of Neutrophil Cytosol Factor 1 Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-Positive, Type 1 Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-Positive, Type I Cgd, Autosomal Recessive Cytochrome B-Positive, Type I Granulomatous Disease, Chronic, Due To Ncf1 Deficiency Neutrophil Cytosol Factor 1 Deficiency Chronic Granulomatous Disease 1, Autosomal Recessive Autosomal Recessive Chronic Granulomatous Disease 1 Cdg1 Deficiency Of Ncf1 Deficiency Of P47-Phox Deficiency Of Soc2 Deficiency Of Soluble Oxidase Component Ii Chronic Granulomatous Disease Autosomal Recessive Cytochrome B-Positive Type I Chronic Granulomatous Disease Due To Ncf1 Deficiency
Granulomatous Disease, Chronic, X-Linked	CGDX Chronic Granulomatous Disease, X-Linked X-Linked Chronic Granulomatous Disease Cgd Cytochrome B-Negative Granulomatous Disease, Chronic, X-Linked Cdgx X-Linked Chronic Cytochrome B-Negative Granulomatous Disease Chronic Granulomatous Disease Cytochrome B-Negative X-Linked Chronic Granulomatous Disease Cytochrome B-Positive X-Linked Granulomatous Disease, Chronic, X-Linked, Variant
Lung Abscess	Apical Lung Abscess Abscess Of Lung Abscess Of Lung Nos Pulmonary Abscess Multiple Abscess Of Lung
Suppurative Lymphadenitis	Suppurative Lymphadenopathy
Splenic Abscess
Ectodermal Dysplasia	Congenital Ectodermal Defect Congenital Ectodermal Dysplasia Ectodermal Dysplasia Syndrome Dysplasia, Ectodermal
Gastrointestinal Defects And Immunodeficiency Syndrome 1	Familial Intestinal Polyatresia Syndrome Fipa Gastrointestinal Defects And Immunodeficiency Syndrome Multiple Gastrointestinal Atresias Familial Isolated Pituitary Adenoma Intestinal Atresia, Multiple Multiple Intestinal Atresia GIDID1 Multiple Intestinal Atresia And/Or Inflammatory Bowel Disease With Or Without Immunodeficiency Minat Gidid Meddra:10028210 Familial Isolated Pituitary Adenoma Syndrome Intestinal Atresia Multiple Combined Immunodeficiency-Enteropathy Spectrum Cid-Mia/Early-Onset Ibd Intestinal Atresia, Multiple And/Or Inflammatory Bowel Disease With Or Without Immunodeficiency Mia Gastrointestinal Defect And Immunodeficiency Syndrome Pituitary Adenoma Predisposition Pituitary Adenoma, Familial Isolated
Lung Disease	Lung Diseases Disorder Of Lung Abnormality Of The Lung
Malignant Secondary Hypertension
Hypertension, Essential	Essential Hypertension Hypertension High Blood Pressure Hypertension, Essential, Susceptibility To Hypertensive Disease Primary Hypertension EHT Hypertension, Salt-Sensitive Essential, Susceptibility To Hyperpiesia Idiopathic Hypertension Hypertensive Disorder Hypertension, Essential, Susceptibility To, 3 Hypertension, Essential 3 Hypertension, Essential, Salt-Sensitive Hypertension, Essential, Susceptibility To, 6 Hypertension, Essential 6 Hypertension, Salt-Sensitive Essential Hypertension, Susceptibility To Hypertension, Essential, Susceptibility To, 4 Hypertension, Essential 4 Hypertension, Essential, Susceptibility To, 2 Hypertension, Essential 2 Hypertension, Essential, Susceptibility To, 1 Hypertension, Essential 1 Hypertension, Essential, Susceptibility To, 5 Hypertension, Essential 5 Htn Vascular Hypertensive Disorder Systemic Primary Arterial Hypertension Hbp - [High Blood Pressure] Systemic Arterial Hypertensive Disorder Elevated Blood Pressure Arterial Hypertension Nos Hypertension Nos Benign Hypertension Systemic Arterial Hypertension Systemic Hypertension Artery Htn Benign Htn Vascular Htn Vascular Hypertension Cholesterol Hypertension Cholesterol Htn Idiopathic Htn Malignant Hypertension Malignant Htn Raised Blood Pressure Cardiovascular Hypertension Primary Htn - [Hypertension] High Arterial Tension High Blood Pressure Disorder Ht - [Hypertension] Htn - [Hypertension] Hypertensive Vascular Disease Hypertensive Vascular Degeneration
Alzheimer Disease, Familial, 1	Alzheimer Disease Alzheimer'S Disease Presenile And Senile Dementia AD1 Alzheimer Disease, Susceptibility To Alzheimer Disease, Late-Onset, Susceptibility To Alzheimer Disease 1, Familial AD Familial Alzheimer Disease Alzheimer Disease, Late-Onset Alzheimers Dementia Alzheimer Dementia Alzheimer Sclerosis Alzheimer Syndrome Alzheimer-Type Dementia Dat Primary Senile Degenerative Dementia Sdat Alzheimer Disease 1 Autosomal Dominant Alzheimer Disease Early-Onset Alzheimer Disease With Cerebral Amyloid Angiopathy Late Onset Alzheimer Disease Alzheimers Disease Alzheimer Disease, Early-Onset, With Cerebral Amyloid Angiopathy Late-Onset Alzheimers Disease Alzheimer'S Disease Pathway Kegg Dementia Due To Alzheimer'S Disease Alzheimer Disease Type 1 Alzheimers

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS09078	NCF2 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS19358	Ncf2 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS25849	Ncf2 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Bos taurus	NCF2	VGNC	VGNC:31912
Felis catus	NCF2	VGNC	VGNC:68425
Rattus norvegicus	NCF2	RGD	RGD:1309424
Macaca mulatta	NCF2	VGNC	VGNC:75140
Mus musculus	NCF2	MGD	MGI:97284
Canis familiaris	NCF2	VGNC	VGNC:43650
Others	NCF2	NCBI