p67phox Protein, Human (sf9, His-GST)

p67phox is central to the activation of NADPH oxidase, which is critical for superoxide production. p67phox forms heterodimers with CYBA and CYBB and interacts with NCF4 and NCF1 to establish important connections within the complex. p67phox Protein, Human (sf9, His-GST) is the recombinant human-derived p67phox protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The p67phox protein plays a pivotal role in the activation of the latent NADPH oxidase, a process essential for superoxide production. As a key component of the NADPH oxidase complex, it forms a heterodimer with the membrane proteins CYBA and CYBB, alongside the cytosolic subunits NCF1, NCF2, and NCF4. Notably, p67phox interacts with NCF4 and NCF1, establishing crucial connections within the complex through its C-terminal SH3 domain. Additionally, it engages with SYTL1 and RAC1 and may interact with NOXO1, highlighting its versatility in associating with regulatory elements. Further interactions with S100A8, calprotectin (S100A8/9), and GBP7 underscore the intricate network involved in the regulation of NADPH oxidase activity. The interaction with CYBB is enhanced in the presence of GBP7, emphasizing the dynamic nature of these protein-protein associations in orchestrating superoxide generation.

Human

Sf9 insect cells

N-His;N-GST

P19878 (M1-V526)

4688  [NCBI]

Full Length

MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGKLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV

80-90 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM Nacl, 10% glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.