Next-generation sequencing to generate interactome datasets

  • Nat Methods. 2011 Jun;8(6):478-80. doi: 10.1038/nmeth.1597.
Haiyuan Yu  1 ,  Leah Tardivo ,  Stanley Tam ,  Evan Weiner ,  Fana Gebreab ,  Changyu Fan ,  Nenad Svrzikapa ,  Tomoko Hirozane-Kishikawa ,  Edward Rietman ,  Xinping Yang ,  Julie Sahalie ,  Kourosh Salehi-Ashtiani ,  Tong Hao ,  Michael E Cusick ,  David E Hill ,  Frederick P Roth ,  Pascal Braun ,  Marc Vidal
Affiliations
  • 1. Center for Cancer Systems Biology, Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
PMID: 21516116 DOI: 10.1038/nmeth.1597
Abstract

Next-generation Sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse Sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation Sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.