p67phox Protein, Human (sf9)
p67phox is central to the activation of NADPH oxidase, which is critical for superoxide production. p67phox forms heterodimers with CYBA and CYBB and interacts with NCF4 and NCF1 to establish important connections within the complex. p67phox Protein, Human (sf9) is the recombinant human-derived p67phox protein, expressed by Sf9 insect cells, with tag free and with additional two amino acids (Gly & Pro) at the N-terminus.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
p67phox is central to the activation of NADPH oxidase, which is critical for superoxide production. p67phox forms heterodimers with CYBA and CYBB and interacts with NCF4 and NCF1 to establish important connections within the complex. p67phox Protein, Human (sf9) is the recombinant human-derived p67phox protein, expressed by Sf9 insect cells, with tag free and with additional two amino acids (Gly & Pro) at the N-terminus.
The p67phox protein plays a pivotal role in the activation of the latent NADPH oxidase, a process essential for superoxide production. As a key component of the NADPH oxidase complex, it forms a heterodimer with the membrane proteins CYBA and CYBB, alongside the cytosolic subunits NCF1, NCF2, and NCF4. Notably, p67phox interacts with NCF4 and NCF1, establishing crucial connections within the complex through its C-terminal SH3 domain. Additionally, it engages with SYTL1 and RAC1 and may interact with NOXO1, highlighting its versatility in associating with regulatory elements. Further interactions with S100A8, calprotectin (S100A8/9), and GBP7 underscore the intricate network involved in the regulation of NADPH oxidase activity. The interaction with CYBB is enhanced in the presence of GBP7, emphasizing the dynamic nature of these protein-protein associations in orchestrating superoxide generation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
P19878 (N-G&P, M1-V526)
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Molecular Construction
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N-term
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Gly-Pro
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p67phox (M1-V526)
Accession # P19878 -
C-term
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Protein Length
Full Length
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Synonyms
NCF2; Chronic Granulomatous Disease, Autosomal 2; Neutrophil Cytosolic Factor 2; 67 KDa Neutrophil Oxidase Factor; NOXA2; NCF-2; Neutrophil NADPH Oxidase Factor 2; Neutrophil Cytosolic Factor 2 Isoform 3; Neutrophil Cytosol Factor 2; P67-PHOX; NADPH Oxida
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Predicted Molecular Mass
59.9 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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