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  4. Janelia Fluor® 646, Azide

Janelia Fluor® 646, Azide  (Synonyms: JF646, Azide)

Cat. No.: HY-131027 Purity: 99.87%
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Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

Janelia Fluor® 646, Azide Chemical Structure

Janelia Fluor® 646, Azide Chemical Structure

Size Price Stock Quantity
1 mg USD 256 In-stock
2 mg USD 410 In-stock
5 mg USD 870 In-stock
10 mg   Get quote  
50 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Janelia Fluor® 646, Azide:

Janelia Fluor® 646, Azide Related Antibodies

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Description

Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments[1][2]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

In Vitro

Maximum absorption wavelength (λabs)= 646 nm, maximum emission wavelength (λem)=664 nm[1].
JF646 can serve as a ligand for self-labeling tag (such as HaloTag)[1].
Janelia Fluor 646, Azide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Janelia Fluor® 646, Azide Related Antibodies
Molecular Weight

696.87

Formula

C37H44N6O6Si
Appearance

Solid

Color

Light green to green

Emission (Em)

646
Excitation (Ex)

605
SMILES

O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CCC4)=C(C3=C/5)C=CC5=[N+]6CCC/6)=C1)C([O-])=O)NCCOCCOCCOCCN=[N+]=[N-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (143.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4350 mL 7.1749 mL 14.3499 mL
5 mM 0.2870 mL 1.4350 mL 2.8700 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4350 mL 7.1749 mL 14.3499 mL 35.8747 mL
5 mM 0.2870 mL 1.4350 mL 2.8700 mL 7.1749 mL
10 mM 0.1435 mL 0.7175 mL 1.4350 mL 3.5875 mL
15 mM 0.0957 mL 0.4783 mL 0.9567 mL 2.3916 mL
20 mM 0.0717 mL 0.3587 mL 0.7175 mL 1.7937 mL
25 mM 0.0574 mL 0.2870 mL 0.5740 mL 1.4350 mL
30 mM 0.0478 mL 0.2392 mL 0.4783 mL 1.1958 mL
40 mM 0.0359 mL 0.1794 mL 0.3587 mL 0.8969 mL
50 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7175 mL
60 mM 0.0239 mL 0.1196 mL 0.2392 mL 0.5979 mL
80 mM 0.0179 mL 0.0897 mL 0.1794 mL 0.4484 mL
100 mM 0.0143 mL 0.0717 mL 0.1435 mL 0.3587 mL
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Janelia Fluor® 646, Azide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 646, AzideJF646, AzideFluorescent DyeclickchemistrygroupAzidefluorescentimagingSTEDInhibitorinhibitorinhibit

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