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United States

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製品番号: HY-P11904

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LADW

LADW is a tetrapeptide derived from the hydrolysate of tuna skeletal muscle myosin, with dual inhibitory activities against angiotensin-converting enzyme 1 (ACE1) and α-glucosidase. The IC₅₀ value of LADW against ACE1 is 28.02 μM, while its IC₅₀ value against Saccharomyces cerevisiae α-glucosidase is 4.40 mM. LADW binds competitively to the active site of ACE1, stabilizes the enzyme conformation, and blocks substrate binding. LADW binds to α-glucosidase to inhibit carbohydrate hydrolysis and can also alter starch structure. LADW can be used in studies related to hypertension and diabetes.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

分子式: C₂₄H₃₃N₅O₇
分子量:503.55

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

カスタムペプチド

LADW 構造式

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

OthersCardiovascular RegulationLocal Vascular RegulationCarbohydrate Metabolism

Others

LADW is a competitive inhibitor of purified ACE1, which binds to the active site of the enzyme or its adjacent region to block substrate binding. It potently inhibits purified ACE1 with an IC₅₀ of 28 μM, exhibiting a concentration-dependent inhibitory effect^[1].

Cardiovascular Regulation

Local Vascular Regulation

LADW (150-300 μM; 24 h) promotes NO release and inhibits ET-1 secretion in EA.hy926 vascular endothelial cells in a concentration-dependent manner^[1].
LADW forms strong interactions with eNOS, ECE-1 and Furin via hydrogen bonds and van der Waals forces and binds to the key active residues of these proteins according to molecular docking results^[1].

Carbohydrate Metabolism

LADW (15 min pre-incubation followed by 15 min reaction) potently inhibits α-glucosidase derived from Saccharomyces cerevisiae, with an IC₅₀ value of 4.40 mM, and its inhibitory activity is stronger than that of EEAEGT^[2].
LADW inhibits the hydrolysis of soluble starch during simulated gastrointestinal digestion by altering starch structure, reducing the production of reducing sugars by 39.56%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

EA.hy926 vascular endothelial cells
Concentration:

150 μM, 300 μM
Incubation Time:

24 h
Result:

Increased NO release by 45.88% (150 μM) and 78.94% (300 μM) compared to controls.
Reduced ET-1 secretion by 22.34% (150 μM) and 60.79% (300 μM) compared to controls.

Related Protocols :

化学情報

分子量 503.55
分子式 C₂₄H₃₃N₅O₇
配列

Leu-Ala-Asp-Trp
シーケンスの短縮

LADW
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Xiong W Y, et al. Effects and mechanisms of a novel peptide LADW on angiotensin I‑converting enzyme and vascular function and its gastrointestinal stability: an in vitro and in silico study. Food Bioscience, 2025, 71: 107345.

[2]. Zhao S, et al. Exploring the Blood Glucose-Lowering Potential of the Umami Peptides LADW and EEAEGT Derived from Tuna Skeletal Myosin: Perspectives from α-Glucosidase Inhibition and Starch Interaction. Foods (Basel, Switzerland). 2024 Jan 17;13(2):294.

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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分子量分子量

希釈計算機

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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