Cat. No.: HY-171047

Autophagy inducer 7

고객리뷰

Based on 1 Customer Validation

Autophagy inducer 7 (Compound SSA) is an Autophagy and Apoptosis inducer. Autophagy inducer 7 activates autophagy by inhibiting Akt/mTOR signaling and the expression of downstream proteins. Autophagy inducer 7 suppresses DNA synthesis and causes a G0-G1 cell-cycle arrest. Autophagy inducer 7 inhibits tumor cell growth.

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Purity: 99.45%
CAS No.: 944159-20-0
화학식: C₂₄H₂₇FN₂OS
분자량:410.55

보관:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Autophagy inducer 7 Chemical Structure

CAS No.: 944159-20-0

사이즈

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재고

수량

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All DNA/RNA Synthesis Isoforms :

All Akt Isoforms :

All mTOR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	EC₅₀	>7.57 μM Compound: 1; SSA	Growth inhibition of human BJ cells after 72 hrs by CellTiter-Glo reagent based assay Growth inhibition of human BJ cells after 72 hrs by CellTiter-Glo reagent based assay	[PMID: 28935266]
HT-29	CC₅₀	0.65 μM Compound: 1; SSA	Growth inhibition of human HT-29 cells after 72 hrs by CellTiter-Glo reagent based assay Growth inhibition of human HT-29 cells after 72 hrs by CellTiter-Glo reagent based assay	[PMID: 28935266]
HT-29	CC₅₀	0.65 μM Compound: SSA	Growth inhibition of human HT-29 cells measured after 72 hrs by Celltiter-Glo reagent based assay Growth inhibition of human HT-29 cells measured after 72 hrs by Celltiter-Glo reagent based assay	[PMID: 29776741]
HT-29	IC₅₀	11.3 μM Compound: 36	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 22940705]
MDA-MB-231	CC₅₀	2.67 μM Compound: 1; SSA	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Glo reagent based assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Glo reagent based assay	[PMID: 28935266]
MDA-MB-231	CC₅₀	2.67 μM Compound: SSA	Growth inhibition of human MDA-MB-231 cells measured after 96 hrs by Celltiter-Glo reagent based assay Growth inhibition of human MDA-MB-231 cells measured after 96 hrs by Celltiter-Glo reagent based assay	[PMID: 29776741]
PC-3	CC₅₀	3.12 μM Compound: 1; SSA	Growth inhibition of human PC3 cells after 72 hrs by CellTiter-Glo reagent based assay Growth inhibition of human PC3 cells after 72 hrs by CellTiter-Glo reagent based assay	[PMID: 28935266]
PC-3	CC₅₀	3.12 μM Compound: SSA	Growth inhibition of human PC3 cells measured after 72 hrs by Celltiter-Glo reagent based assay Growth inhibition of human PC3 cells measured after 72 hrs by Celltiter-Glo reagent based assay	[PMID: 29776741]

In Vitro

Autophagy inducer 7 (72 h) inhibits the growth of human lung adenocarcinoma cell lines, with IC₅₀s of 1.95 μM (HOP-62), 4.72 μM (A549), 3.05μM (H1299)^[1].
Autophagy inducer 7 (0-10 μM, 24 h) induces apoptosis in HOP-62, A549, H1299 cells, but not significant as Sulindac sulfide (HY-B1786) and Staurosporine (HY-15141)^[1].
Autophagy inducer 7 (0-7.5 μM, 24 h) inhibits DNA synthesis in HOP-62, A549, H1299 cells, and increases the percentage of cells in the G0-G1 phase of the cell cycle^[1].
Autophagy inducer 7 (0-7.5 μM, 24 h) induces autophagy and increases autophagic flux by inhibiting Akt/mTOR/p70S6k signaling in A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

HOP-62, A549, H1299 cells
Concentration:

2.5, 5, 7.5, 10 μM
Incubation Time:

24 h
Result:

Induced caspase3/7 activation dose-dependently.
Induced cleaved PARP expression at 10 μM.
Increased Annexin-V surface staining and PI labeling.

Cell Autophagy Assay^[1]

Cell Line:

HOP-62, A549, H1299 cells
Concentration:

2.5, 5, 7.5, 10 μM
Incubation Time:

24 h
Result:

Induced dose- and time-dependent cleavage of cytosolic LC3-I to autophagosome-associated LC3-II.
Decreased p62 expression.
Displayed a striking accumulation of vesicle-like structures within the cytoplasm.
Increased both the number and size of acidic vesicles within the cytoplasm.

Chemical Information

CAS No. 944159-20-0
Appearance Solid
분자량 410.55
화학식 C₂₄H₂₇FN₂OS
Color Light yellow to yellow
SMILES

CN(C)CCNC(CC1=C(C)/C(C2=CC=C(F)C=C21)=C/C3=CC=C(C=C3)SC)=O
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

용액&용해도

In Vitro:

DMSO : 50 mg/mL (121.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4358 mL

12.1788 mL

24.3576 mL

5 mM

0.4872 mL

2.4358 mL

4.8715 mL

10 mM

0.2436 mL

1.2179 mL

2.4358 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. 1. Gurpinar E, et al. A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy. Mol Cancer Ther. 2013 May;12(5):663-74. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4358 mL	12.1788 mL	24.3576 mL	60.8939 mL
	5 mM	0.4872 mL	2.4358 mL	4.8715 mL	12.1788 mL
	10 mM	0.2436 mL	1.2179 mL	2.4358 mL	6.0894 mL
	15 mM	0.1624 mL	0.8119 mL	1.6238 mL	4.0596 mL
	20 mM	0.1218 mL	0.6089 mL	1.2179 mL	3.0447 mL
	25 mM	0.0974 mL	0.4872 mL	0.9743 mL	2.4358 mL
	30 mM	0.0812 mL	0.4060 mL	0.8119 mL	2.0298 mL
	40 mM	0.0609 mL	0.3045 mL	0.6089 mL	1.5223 mL
	50 mM	0.0487 mL	0.2436 mL	0.4872 mL	1.2179 mL
	60 mM	0.0406 mL	0.2030 mL	0.4060 mL	1.0149 mL
	80 mM	0.0304 mL	0.1522 mL	0.3045 mL	0.7612 mL
	100 mM	0.0244 mL	0.1218 mL	0.2436 mL	0.6089 mL

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Autophagy inducer 7

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

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Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

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Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

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Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

All DNA/RNA Synthesis Isoforms

All Akt Isoforms

All mTOR Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Autophagy inducer 7 Related Classifications

HY-171047 Related Classifications