L-Asparaginase
Based on 16 publication(s) in Google Scholar
L-Asparaginase (L-ASNase) is a deamidating enzyme that catalyses the hydrolysis of L-asparagine and L-glutamine, and can be used for the research of acute lymphoblastic leukemia. L-Asparaginase depletes L-asparagine from plasma resulting in inhibition of RNA and DNA synthesis with the subsequent blastic cell apoptosis.
For research use only. We do not sell to patients.
- CAS No.: 9015-68-3
- Molecular Weight:34000 (monomer)
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Asparaginase
More- Nat Commun. 2025 Jul 1;16(1):5675. [Abstract]
- Adv Sci (Weinh). 2026 Mar 20:e16557. [Abstract]
- Sci Adv. 2025 Jun 20;11(25):eadt3075. [Abstract]
- Mol Ther. 2025 Aug 12:S1525-0016(25)00650-1. [Abstract]
- Cell Rep Med. 2025 Apr 25:102098. [Abstract]
- Cell Death Dis. 2023 Aug 1;14(8):489. [Abstract]
- EMBO Mol Med. 2023 Feb 8;15(2):e16554. [Abstract]
- Aging Cell. 2025 Oct;24(10):e70203. [Abstract]
- Cell Rep. 2025 Nov 25;44(11):116496. [Abstract]
- Biochem Pharmacol. 2023 Sep:215:115690. [Abstract]
- Cancers (Basel). 2022 Oct 19;14(20):5127. [Abstract]
- Front Mol Neurosci. 2020 Sep 30;13:563054. [Abstract]
- BMC Cancer. 2022 Jul 2;22(1):724. [Abstract]
- Front Oncol. 2024 Jul 19:14:1440650. [Abstract]
- Blood Neoplasia. 2026 Apr 9;3(3):100230. [Abstract]
- bioRxiv. 2026 Mar 18.
Customer Validation & Images
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Cell Migration/Invasion Assay
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IHC
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In Vivo Efficacy Study
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
All DNA/RNA Synthesis Isoforms
More
Biological Activity
L-Asparaginase has a cytotoxic effect on AML blasts and LSC subpopulations[1].
L-Asparaginase is effective on both CD34+CD38+ and CD34+CD38+ LSC fractions, MSC and monocytes/macrophages partially counteracted the effect[1].
Although lyophilized L-asparaginase is stable at room temperature for 3 weeks, it should be stored dry below -18°C. L-Asparaginase should Co-culture with cells at 37°C, 5% CO2 for 24, 48 h.
L-Asparaginase (100 IU/mL, 0-24 h) is co-cultured with L5178Y and B16 cells (5×105/mL), and cells are arrested in the G1 phase and DNA strand breaks occurred[2].
Product Information
Optimal pH: 8.0-9.0
Agonists: Anaerobic conditions, amino acids
Inhibitors: 5-diazo-4-o-L-norvaline (DONV), dimethyl sulfoxide, glutaraldehyde, L-asparagine and L-aspartic acid, NH4+, trypsin
Instructions
Dissolve in 1 mg/ml 0.2 M Tris-HCl pH 8.6, store at 2-8°C after dissolution, and use as soon as possible within 1 week. Or dissolve according to specific experimental references.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
3.5.1.1
100 - 300 U/mg protein
One unit will release 1.0μmol of ammonia from L-asparagine per minute at pH8.5 and 37°C.
Chemical Information
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CAS No. 9015-68-3
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Appearance Solid
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Molecular Weight 34000 (monomer)
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Color White to off-white
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SMILES
[L-Asparaginase]
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Synonyms
L-ASNase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (16)
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Journal Impact Factor
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Most Recent
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Nat Commun
2025 Jul 1;16(1):5675. PMID: 40593710 -
Adv Sci (Weinh)
ETV4 Promotes Colorectal Cancer Progression by Reprogramming Asparagine Metabolism to Remodel the Stromal Microenvironment. [Abstract]2026 Mar 20:e16557. PMID: 41861091 -
Sci Adv
FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production. [Abstract]2025 Jun 20;11(25):eadt3075. PMID: 40531994
L-Asparaginase purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jun 20;11(25):eadt3075. [Abstract]
Transwell migration assay of indicated cells in the presence of ʟ-Asn(50 μg/ml) or ʟ-ASNase (L-Asparaginase; 10 μg/mL; 24-36 h).
L-Asparaginase purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jun 20;11(25):eadt3075. [Abstract]
Brain slices of mice were stained with hematoxylin andeosin (H&E) at the endpoint. BCBM model mice, either cisplatin (4 mg/kg, intraperitoneally) or ʟ-ASNase (L-Asparaginase; 0.2 mg per mouse, intraperitoneally) was administered weekly or every 2 days for 2 weeks, respectively.
L-Asparaginase purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jun 20;11(25):eadt3075. [Abstract]
BLI was conducted at regular intervals. Representative BLI images and line chart of radiance. BCBM model, Mice were administered cisplatin (4 mg/kg, intraperitoneally) weekly or ʟ-ASNase (0.2 mg per mouse, intraperitoneally) every 2 days.
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Mol Ther
Asparaginase enhances CAR-T cell antitumor immunity by asparagine metabolic reprogramming and central memory induction in ALL. [Abstract]2025 Aug 12:S1525-0016(25)00650-1. PMID: 40808257 -
Cell Rep Med
scRNA-seq reveals an immune microenvironment and JUN-mediated NK cell exhaustion in relapsed T-ALL. [Abstract]2025 Apr 25:102098. PMID: 40306275 -
Cell Death Dis
NUCKS1, a LINC00629-upregulated gene, facilitated osteosarcoma progression and metastasis by elevating asparagine synthesis. [Abstract]2023 Aug 1;14(8):489. PMID: 37528150
L-Asparaginase purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Aug 1;14(8):489. [Abstract]
MNNG/HOS cells with or without NUCKS1 depletion were treated with 10 μM L-Asparaginase for 48 h. Cell death was measured by PI staining.
L-Asparaginase purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Aug 1;14(8):489. [Abstract]
MNNG/HOS cells with or without NUCKS1 depletion were treated with 10 μM L-Asparaginase for 48 h. Cell death was measured by PI staining.
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EMBO Mol Med
β-Catenin activity induces an RNA biosynthesis program promoting therapy resistance in T-cell acute lymphoblastic leukemia. [Abstract]2023 Feb 8;15(2):e16554. PMID: 36597789 -
Aging Cell
Asparaginase and Autophagy Inhibitors Effectively Remove Senescent Cells by Synergistically Limiting Asparagine Supply. [Abstract]2025 Oct;24(10):e70203. PMID: 40905331 -
Cell Rep
A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential. [Abstract]2025 Nov 25;44(11):116496. PMID: 41166305 -
Biochem Pharmacol
Methylseleninic acid overcomes gefitinib resistance through asparagine-MET-TOPK signaling axis in non-small cell lung cancer cells. [Abstract]2023 Sep:215:115690. PMID: 37481142 -
Cancers (Basel)
Association between Dysfunction of the Nucleolar Stress Response and Multidrug Resistance in Pediatric Acute Lymphoblastic Leukemia. [Abstract]2022 Oct 19;14(20):5127. PMID: 36291909 -
Front Mol Neurosci
L-Asparaginase Exerts Neuroprotective Effects in an SH-SY5Y-A53T Model of Parkinson's Disease by Regulating Glutamine Metabolism. [Abstract]2020 Sep 30;13:563054. PMID: 33117129 -
BMC Cancer
2022 Jul 2;22(1):724. PMID: 35778693 -
Front Oncol
Hypoxanthine in the microenvironment can enable thiopurine resistance in acute lymphoblastic leukemia. [Abstract]2024 Jul 19:14:1440650. PMID: 39099696 -
Blood Neoplasia
From cell lines to PDXs: in vivo confirmation of synergistic drug responses identified in leukemia cell line models. [Abstract]2026 Apr 9;3(3):100230. PMID: 42294111 -
Solvent & Solubility
H2O : 100 mg/mL (Need ultrasonic)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL; Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (271 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Ilaria M Michelozzi, et al. Acute myeloid leukaemia niche regulates response to L-asparaginase. Br J Haematol. 2019 Aug;186(3):420-430. doi: 10.1111/bjh.15920. [Content Brief]
[2]. T Ueno, et al. Cell cycle arrest and apoptosis of leukemia cells induced by L-asparaginase. Leukemia. 1997 Nov;11(11):1858-61. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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