Lipase,Aspergillusniger (Immobilized)
Based on 1 Customer Validation
Lipase, Aspergillus niger (Immobilized) is an immobilized triacylglycerol acyl hydrolase and biocatalyst. Lipase, Aspergillus niger (Immobilized) broadly catalyzes the hydrolysis of glycerol-long-chain fatty acid esters, exhibits positional selectivity for the 1,3-positions of glycerol, but shows low hydrolysis efficiency for castor oil. Lipase, Aspergillus niger (Immobilized) displays optimal activity at 45°C and pH 7.0, and remains stable at temperatures up to 60°C and over a broad pH range of 2.0-9.0. Lipase, Aspergillus niger (Immobilized) is activated by Ca2+ and Mg2+, but inhibited by Mn2+, Fe2+ and Zn2+.
For research use only. We do not sell to patients.
- CAS No.: 9001-62-1
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
In Vitro
Lipase,Aspergillusniger (Immobilized) remains stable for 24 hours in 40% (V/V) methanol, 2-propanol, and methyl acetate. It tolerates high concentrations of water-miscible organic solvents, exhibits no relevant protease activity, and shows enhanced activity after pre-incubation at 37 or 40 °C in a medium supplemented with olive oil[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 9001-62-1
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Appearance Solid
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Color Yellow to brown
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SMILES
[Lipase,Aspergillusniger (Immobilized)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
H2O : ≥ 3.33 mg/mL
* "≥" means soluble, but saturation unknown.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)