Lipase,Pseudomonas fluorescens (Immobilized)
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Lipase, Pseudomonas fluorescens (Immobilized) is an immobilized biocatalyst derived from Pseudomonas fluorescens. Lipase, Pseudomonas fluorescens (Immobilized) efficiently catalyzes the hydrolysis, esterification and transesterification of triacylglycerols in solvent-free systems, and is particularly suitable for biodiesel production from soybean oil and selective modification of oils rich in EPA and DHA. Lipase, Pseudomonas fluorescens (Immobilized) exhibits optimal activity at pH 8.5 and 45°C, and retains residual activity after repeated cycles of use. Lipase, Pseudomonas fluorescens (Immobilized) is activated by Ca2+, but inhibited by Co2+, Ni2+, Fe3+, Fe2+ and EDTA.
For research use only. We do not sell to patients.
- CAS No.: 9001-62-1
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Lipase, Pseudomonas fluorescens (Immobilized) Pfip1a (0.19 mg-protein/mL; 2h) (on magnetic nanoparticles) has an 84.2% immobilization efficiency and 182.5% activity recovery, with activity increased 1.8-fold compared to free enzyme[1].
Lipase, Pseudomonas fluorescens (Immobilized) Pfip1a (10 wt%; 42h at 60°C, 24h at 70°C) catalyzes transesterification of soybean oil to biodiesel with an 80.5% yield after 24h at 70°C, and completes a full 42h transesterification reaction at 60°C[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9001-62-1
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Appearance Solid
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Color Light yellow to light brown
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SMILES
[Lipase,Pseudomonas fluorescens (Immobilized)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu W, et al. Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0-1 and used for biodiesel production. Sci Rep. 2017;7(1):15711. Published 2017 Nov 16. [Content Brief]
[2]. Kojima Y, et al. Purification and characterization of the lipase from Pseudomonas fluorescens HU380. J Biosci Bioeng. 2003;96(3):219-226. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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