Cat. No.: HY-N0351

p-Coumaric acid

(Synonyms: trans-4-Hydroxycinnamic acid)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

p-Coumaric acid (trans-4-Hydroxycinnamic acid) is an isomer of cinnamic acid with oral activity. p-Coumaric acid inhibits cell proliferation and promotes apoptosis. p-Coumaric acid has antibacterial, anti-inflammatory, antioxidant and anti-tumor activities.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 501-98-4
Formula: C₉H₈O₃
Molecular Weight:164.16

Storage:

RT, stored under nitrogen.

In solvent -80°C, 1 year , -20°C, 6 months

p-Coumaric acid Chemical Structure

CAS No.: 501-98-4

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
A549	IC₅₀	>10 μM Compound: 6	Cytotoxicity against human A549 cells after 48 hrs by SRB assay Cytotoxicity against human A549 cells after 48 hrs by SRB assay	[PMID: 27700070]
B16-F10	IC₅₀	>10 μM Compound: PCA	Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
BT-549	IC₅₀	10 μM Compound: 6	Cytotoxicity against human BT549 cells after 48 hrs by SRB assay Cytotoxicity against human BT549 cells after 48 hrs by SRB assay	[PMID: 27700070]
BV-2	IC₅₀	>50 μM Compound: 6	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 27700070]
BV-2	IC₅₀	>100 μM Compound: 23	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 28911817]
HepG2	IC₅₀	>10 μM Compound: PCA	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
HT-1080	ED₅₀	>100 μM Compound: 18	Antiproliferative activity against human HT1080 cells by MTT assay Antiproliferative activity against human HT1080 cells by MTT assay	[PMID: 11277741]
HT-22	EC₅₀	6.4 μM Compound: 29	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
HT-29	IC₅₀	>10 μM Compound: PCA	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
KB	IC₅₀	>200 μM Compound: 1f	Antitumor activity against KB cells by MTT assay Antitumor activity against KB cells by MTT assay	[PMID: 17387015]
LoVo	IC₅₀	82 μM Compound: 14	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
Oocyte	IC₅₀	9.59 μM Compound: 4-OH-Cin-COOH	Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique	[PMID: 23682762]
PC-3	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
Platelet	IC₅₀	74.8 μM Compound: 4-OH-cinnamic acid	Anti-platelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins at 37 degC by aggregometry Anti-platelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins at 37 degC by aggregometry	10.1039/C4MD00022F
RAW264.7	IC₅₀	>50 μM Compound: 11	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 22079762]
RAW264.7	IC₅₀	73.4 μM Compound: 11	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25666824]
RAW264.7	GI₅₀	4560 μM Compound: 1a	Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay	10.1039/C3MD00251A
SK-MEL-2	IC₅₀	>10 μM Compound: 6	Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay	[PMID: 27700070]
SK-MEL-28	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
SK-OV-3	IC₅₀	>10 μM Compound: 6	Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay	[PMID: 27700070]
U-373MG ATCC	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-937	CC₅₀	>2000 μM Compound: 24, p-coumaric acid	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]
U-937	IC₅₀	>2000 μM Compound: 24, p-coumaric acid	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]

In Vitro

p-Coumaric acid (1 or 3 μg/mL, 24, 48, 72 h) can significantly inhibit the proliferation of human and mouse melanoma cells in vitro and promote cell apoptosis^[1].
p-Coumaric acid (10-80 μg/mL) shows antibacterial activity against both gram-negative and Gram-positive bacteria, and MIC values for Escherichia coli, Streptococcus dysenteriae and Salmonella typhimurium are 80, 10 and 20 μg/ml^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

A375, B16
Concentration:

1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5 mM
Incubation Time:

24, 48 h
Result:

Inhibited the proliferation of A375 and B16 cells The IC₅₀ values on A375 cells were 4.4 mM and 2.5 mM , and the IC₅₀ values on B16 cells were 4.1 mM and 2.8 mM in 24 and 48 h, respectively.

Cell Cycle Analysis^[1]

Cell Line:

A375, B16
Concentration:

1.5, 2.5, 3 mM;2, 3, 4 mM
Incubation Time:

24 h
Result:

Increased the S phase proportion in A375 cells and the G0/G1 phase proportion in B16 cells.

Western Blot Analysis^[1]

Cell Line:

A375, B16
Concentration:

1.5, 2.5, 3 mM;2, 3, 4 mM
Incubation Time:

24 h
Result:

Reduced the expression levels of CDK2 and Cyclin A in A375 cells and the levels of CDK2 and Cyclin E in B16 cells.
Decreased the levels of caspase-3 and caspase-9 and increased the levels of cleaved caspase-3 and cleaved caspase-9.
Downregulated Bcl-2 and upregulated Bax, Apaf1, and cytoplasmic Cyto-C levels.

In Vivo

p-Coumaric acid (50, 100, 200 mg/kg, suspended in 0.5% carboxymethyl cellulose (CMC), was administered daily via gastric cannula for 15 weeks) It has a protective effect on colon pretumor induced by 1,2 dimethylhydrazine (DMH) in rats^[3].
p-Coumaric acid (15, 100 mg/kg, oral) alleviates nephrotoxicity induced by Doxorubicin (HY-15142A) in rats by inhibiting oxidative stress, inflammation and apoptosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

DMH-induced colonic preneoplastic lesions in rats^[3]
Dosage:

50, 100, 200 mg/kg
Administration:

suspended in 0.5% carboxymethylcellulose (CMC) and administered every day via intragastric intubation
Result:

Reduced the polyp incidence to 71.42%, 57.14% and 42.85% respectively.
Reduced the ACF and DACF in a dose-dependent manner and the β– catenin immune activity.
Decreased levels of TBARS and increased levels of SOD, CAT and GPx.
Decreased the activity of β-glucuronidase, and mucinase.

Animal Model:

Doxorubicin-induced nephrotoxicity rats^[4]
Dosage:

15, 100 mg/kg
Administration:

p.o.
Result:

Decreased serum creatinine, BUN and lipid peroxidation, IL-1β and TNF-α.
Decreased the number of TUNEL-positive cells.

Chemical Information

CAS No. 501-98-4
Appearance Solid
Molecular Weight 164.16
Formula C₉H₈O₃
Color Off-white to light yellow
SMILES

O=C(O)/C=C/C1=CC=C(O)C=C1
Synonyms

trans-4-Hydroxycinnamic acid
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Monophenols
Initial Source
- Plants
- other families
- Microorganisms
- Endogenous metabolite
S. cerevisiae strain
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Publications (5)

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Journal Impact Factor

Food Chem

Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]

2023 Mar 30;405(Pt A):134807. PMID: 36370576
Phytomedicine

2026 Jul 25:157:158317. PMID: 42177885
Food Biosci

2024 Nov 28.

p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.

Serum glucose, AST, ALT, TG, and TCHO levels after 12 h of fasting in CHOW or DIO mice. p-Coumaric acid (p-CA, 30 and 100 mg/kg; i.g.; once daily for 12 weeks) significantly reduced blood glucose and NAFLD-related indicators, including AST, ALT, TG and TCHO, in mice induced by a high-fat diet (HFD).

p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.

H&E and Oil Red O (ORO) staining images of the liver in 17-week-old male mice. Scale bar, 200 μm. Histological examination of the liver by H&E staining showed that numerous hepatocytes in the HFD control group presented with fatty degeneration, characterized by small, round vacuoles within the cytoplasm, accompanied by mild hydropic degeneration with swollen, loose, and pale-staining cytoplasm. Following p-Coumaric acid (p-CA, 30 and 100 mg/kg; i.g.; once daily for 12 weeks) administration, the lobular architecture of the liver was clearly defined and arranged regularly without apparent abnormalities.

p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.

The concentration of GLP-1 in the culture medium of STC-1 cells and NCI-H716 cells was determined by ELISA. The results showed that p-Coumaric acid (p-CA, 400 μM; 5 h) effectively enhanced GLP-1 secretion in cells.

p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.

Protein expression of GRP78 in the terminal ileum of the small intestine tissue was detected by Western blot. p-Coumaric acid (p-CA, 30 and 100 mg/kg; i.g.; once daily for 12 weeks; mice) resulted in a significant decrease of GRP78 protein expression compared to the HFD control group, with the high-dose group showing a better effect.
Chem Biol Interact

2026 Jul 25:435:112114. PMID: 42092523
J Nutr Biochem

P-coumaric-acid ameliorates acute kidney injury induced by ischemia-reperfusion injury via suppressing ferroptosis. [Abstract]

2025 Nov 7:149:110175. PMID: 41207539

p-Coumaric acid purchased from MedChemExpress. Usage Cited in: J Nutr Biochem. 2025 Nov 7:149:110175. [Abstract]

HE and Masson staining were used to assess the renal histological morphology and the degree of renal fibrosis in each group of mice. The results showed that p-Coumaric acid (p-CA, 25-100 mg/kg; i.g.; once daily for 2 weeks) significantly protected renal tissue in a mouse model of AKI induced by I/R.

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (152.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (6.09 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.0916 mL

30.4581 mL

60.9162 mL

5 mM

1.2183 mL

6.0916 mL

12.1832 mL

10 mM

0.6092 mL

3.0458 mL

6.0916 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (12.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (12.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (12.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hu X, et al. The Anti-tumor Effects of p-Coumaric Acid on Melanoma A375 and B16 Cells. Front Oncol. 2020 Oct 16;10:558414. [Content Brief]

[2]. NLou Z, et al. p-Coumaric acid kills bacteria through dual damage mechanisms. Food control, 2012, 25(2): 550-554..

[3]. Sharma SH, et al. Protective effect of p-coumaric acid against 1,2 dimethylhydrazine induced colonic preneoplastic lesions in experimental rats. Biomed Pharmacother. 2017 Oct;94:577-588. [Content Brief]

[4]. Rafiee Z, et al Doxorubicin-Induced Nephrotoxicity Through Suppression of Oxidative Stress, Inflammation and Apoptosis. Arch Med Res. 2020 Jan;51(1):32-40. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	6.0916 mL	30.4581 mL	60.9162 mL	152.2904 mL
H₂O / DMSO	5 mM	1.2183 mL	6.0916 mL	12.1832 mL	30.4581 mL
DMSO	10 mM	0.6092 mL	3.0458 mL	6.0916 mL	15.2290 mL
	15 mM	0.4061 mL	2.0305 mL	4.0611 mL	10.1527 mL
	20 mM	0.3046 mL	1.5229 mL	3.0458 mL	7.6145 mL
	25 mM	0.2437 mL	1.2183 mL	2.4366 mL	6.0916 mL
	30 mM	0.2031 mL	1.0153 mL	2.0305 mL	5.0763 mL
	40 mM	0.1523 mL	0.7615 mL	1.5229 mL	3.8073 mL
	50 mM	0.1218 mL	0.6092 mL	1.2183 mL	3.0458 mL
	60 mM	0.1015 mL	0.5076 mL	1.0153 mL	2.5382 mL
	80 mM	0.0761 mL	0.3807 mL	0.7615 mL	1.9036 mL
	100 mM	0.0609 mL	0.3046 mL	0.6092 mL	1.5229 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

p-Coumaric acid

5 Cited Publications

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p-Coumaric acid

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Publications Citing Use of MedChemExpress (MCE) p-Coumaric acid

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

p-Coumaric acid Related Classifications

HY-N0351 Related Classifications