p-Coumaric acid (Synonyms: trans-4-Hydroxycinnamic acid)

Name: p-Coumaric acid
Brand: MedChemExpress
SKU: HY-N0351
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0351 Purity: 99.90%: Data Sheet
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p-Coumaric acid (trans-4-Hydroxycinnamic acid) is an isomer of cinnamic acid with oral activity. p-Coumaric acid inhibits cell proliferation and promotes apoptosis. p-Coumaric acid has antibacterial, anti-inflammatory, antioxidant and anti-tumor activities.

For research use only. We do not sell to patients.

CAS No. : 501-98-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

Other Forms of p-Coumaric acid:

3 Publications Citing Use of MCE p-Coumaric acid

Bio/Physico-chemical Assay

Histological Imaging/Staining

ELISA

: p-Coumaric acid purchased from MedChemExpress. Usage Cited in: J Nutr Biochem. 2025 Nov 7:149:110175. [Abstract]; HE and Masson staining were used to assess the renal histological morphology and the degree of renal fibrosis in each group of mice. The results showed that p-Coumaric acid (p-CA, 25-100 mg/kg; i.g.; once daily for 2 weeks) significantly protected renal tissue in a mouse model of AKI induced by I/R.

: p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.; Serum glucose, AST, ALT, TG, and TCHO levels after 12 h of fasting in CHOW or DIO mice. p-Coumaric acid (p-CA, 30 and 100 mg/kg; i.g.; once daily for 12 weeks) significantly reduced blood glucose and NAFLD-related indicators, including AST, ALT, TG and TCHO, in mice induced by a high-fat diet (HFD).

: p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.; H&E and Oil Red O (ORO) staining images of the liver in 17-week-old male mice. Scale bar, 200 μm. Histological examination of the liver by H&E staining showed that numerous hepatocytes in the HFD control group presented with fatty degeneration, characterized by small, round vacuoles within the cytoplasm, accompanied by mild hydropic degeneration with swollen, loose, and pale-staining cytoplasm. Following p-Coumaric acid (p-CA, 30 and 100 mg/kg; i.g.; once daily for 12 weeks) administration, the lobular architecture of the liver was clearly defined and arranged regularly without apparent abnormalities.

: p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.; The concentration of GLP-1 in the culture medium of STC-1 cells and NCI-H716 cells was determined by ELISA. The results showed that p-Coumaric acid (p-CA, 400 μM; 5 h) effectively enhanced GLP-1 secretion in cells.

: p-Coumaric acid purchased from MedChemExpress. Usage Cited in: Food Biosci. 2024 Nov 28.; Protein expression of GRP78 in the terminal ileum of the small intestine tissue was detected by Western blot. p-Coumaric acid (p-CA, 30 and 100 mg/kg; i.g.; once daily for 12 weeks; mice) resulted in a significant decrease of GRP78 protein expression compared to the HFD control group, with the high-dose group showing a better effect.

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 6	Cytotoxicity against human A549 cells after 48 hrs by SRB assay Cytotoxicity against human A549 cells after 48 hrs by SRB assay	[PMID: 27700070]
A549	IC₅₀	> 100 μM Compound: 14	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
B16-F10	IC₅₀	> 10 μM Compound: PCA	Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
BT-549	IC₅₀	10 μM Compound: 6	Cytotoxicity against human BT549 cells after 48 hrs by SRB assay Cytotoxicity against human BT549 cells after 48 hrs by SRB assay	[PMID: 27700070]
BV-2	IC₅₀	> 100 μM Compound: 23	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 28911817]
BV-2	IC₅₀	> 50 μM Compound: 6	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 27700070]
HT-1080	ED₅₀	> 100 μM Compound: 18	Antiproliferative activity against human HT1080 cells by MTT assay Antiproliferative activity against human HT1080 cells by MTT assay	[PMID: 11277741]
HT-22	EC₅₀	6.4 μM Compound: 29	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
HT-29	IC₅₀	> 10 μM Compound: PCA	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
HepG2	IC₅₀	> 10 μM Compound: PCA	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
KB	IC₅₀	> 200 μM Compound: 1f	Antitumor activity against KB cells by MTT assay Antitumor activity against KB cells by MTT assay	[PMID: 17387015]
LoVo	IC₅₀	82 μM Compound: 14	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
Oocyte	IC₅₀	9.59 μM Compound: 4-OH-Cin-COOH	Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique	[PMID: 23682762]
PC-3	IC₅₀	> 100 μM Compound: 14	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
Platelet	IC₅₀	74.8 μM Compound: 4-OH-cinnamic acid	Anti-platelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins at 37 degC by aggregometry Anti-platelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins at 37 degC by aggregometry	10.1039/C4MD00022F
RAW264.7	GI₅₀	4560 μM Compound: 1a	Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay	10.1039/C3MD00251A
RAW264.7	IC₅₀	73.4 μM Compound: 11	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25666824]
RAW264.7	IC₅₀	> 50 μM Compound: 11	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 22079762]
SK-MEL-2	IC₅₀	> 10 μM Compound: 6	Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay	[PMID: 27700070]
SK-MEL-28	IC₅₀	> 100 μM Compound: 14	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
SK-OV-3	IC₅₀	> 10 μM Compound: 6	Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay	[PMID: 27700070]
U-373MG ATCC	IC₅₀	> 100 μM Compound: 14	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-937	CC₅₀	> 2000 μM Compound: 24, p-coumaric acid	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]
U-937	IC₅₀	> 2000 μM Compound: 24, p-coumaric acid	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]

In Vitro

p-Coumaric acid (1 or 3 μg/mL, 24, 48, 72 h) can significantly inhibit the proliferation of human and mouse melanoma cells in vitro and promote cell apoptosis^[1].
p-Coumaric acid (10-80 μg/mL) shows antibacterial activity against both gram-negative and Gram-positive bacteria, and MIC values for Escherichia coli, Streptococcus dysenteriae and Salmonella typhimurium are 80, 10 and 20 μg/ml^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	A375, B16
Concentration:	1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5 mM
Incubation Time:	24, 48 h
Result:	Inhibited the proliferation of A375 and B16 cells The IC₅₀ values on A375 cells were 4.4 mM and 2.5 mM , and the IC₅₀ values on B16 cells were 4.1 mM and 2.8 mM in 24 and 48 h, respectively.

Cell Cycle Analysis^[1]

Cell Line:	A375, B16
Concentration:	1.5, 2.5, 3 mM;2, 3, 4 mM
Incubation Time:	24 h
Result:	Increased the S phase proportion in A375 cells and the G0/G1 phase proportion in B16 cells.

Western Blot Analysis^[1]

Cell Line:	A375, B16
Concentration:	1.5, 2.5, 3 mM;2, 3, 4 mM
Incubation Time:	24 h
Result:	Reduced the expression levels of CDK2 and Cyclin A in A375 cells and the levels of CDK2 and Cyclin E in B16 cells. Decreased the levels of caspase-3 and caspase-9 and increased the levels of cleaved caspase-3 and cleaved caspase-9. Downregulated Bcl-2 and upregulated Bax, Apaf1, and cytoplasmic Cyto-C levels.

In Vivo

p-Coumaric acid (50, 100, 200 mg/kg, suspended in 0.5% carboxymethyl cellulose (CMC), was administered daily via gastric cannula for 15 weeks) It has a protective effect on colon pretumor induced by 1,2 dimethylhydrazine (DMH) in rats^[3].
p-Coumaric acid (15, 100 mg/kg, oral) alleviates nephrotoxicity induced by Doxorubicin (HY-15142A) in rats by inhibiting oxidative stress, inflammation and apoptosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	DMH-induced colonic preneoplastic lesions in rats^[3]
Dosage:	50, 100, 200 mg/kg
Administration:	suspended in 0.5% carboxymethylcellulose (CMC) and administered every day via intragastric intubation
Result:	Reduced the polyp incidence to 71.42%, 57.14% and 42.85% respectively. Reduced the ACF and DACF in a dose-dependent manner and the β– catenin immune activity. Decreased levels of TBARS and increased levels of SOD, CAT and GPx. Decreased the activity of β-glucuronidase, and mucinase.

Animal Model:	Doxorubicin-induced nephrotoxicity rats^[4]
Dosage:	15, 100 mg/kg
Administration:	p.o.
Result:	Decreased serum creatinine, BUN and lipid peroxidation, IL-1β and TNF-α. Decreased the number of TUNEL-positive cells.

Molecular Weight

164.16

Formula

C₉H₈O₃

CAS No.

501-98-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(O)/C=C/C1=CC=C(O)C=C1

Structure Classification

Initial Source

Microorganisms

Endogenous metabolite

S. cerevisiae strain

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (152.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (6.09 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0916 mL	30.4581 mL	60.9162 mL
5 mM	1.2183 mL	6.0916 mL	12.1832 mL
10 mM	0.6092 mL	3.0458 mL	6.0916 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (12.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (12.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (12.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (278 KB) SDS (396 KB)

COA (244 KB) RP-HPLC (224 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Hu X, et al. The Anti-tumor Effects of p-Coumaric Acid on Melanoma A375 and B16 Cells. Front Oncol. 2020 Oct 16;10:558414. [Content Brief]

[2]. NLou Z, et al. p-Coumaric acid kills bacteria through dual damage mechanisms. Food control, 2012, 25(2): 550-554..

[3]. Sharma SH, et al. Protective effect of p-coumaric acid against 1,2 dimethylhydrazine induced colonic preneoplastic lesions in experimental rats. Biomed Pharmacother. 2017 Oct;94:577-588. [Content Brief]

[4]. Rafiee Z, et al Doxorubicin-Induced Nephrotoxicity Through Suppression of Oxidative Stress, Inflammation and Apoptosis. Arch Med Res. 2020 Jan;51(1):32-40. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.0916 mL	30.4581 mL	60.9162 mL	152.2904 mL
H₂O / DMSO	5 mM	1.2183 mL	6.0916 mL	12.1832 mL	30.4581 mL
DMSO	10 mM	0.6092 mL	3.0458 mL	6.0916 mL	15.2290 mL
	15 mM	0.4061 mL	2.0305 mL	4.0611 mL	10.1527 mL
	20 mM	0.3046 mL	1.5229 mL	3.0458 mL	7.6145 mL
	25 mM	0.2437 mL	1.2183 mL	2.4366 mL	6.0916 mL
	30 mM	0.2031 mL	1.0153 mL	2.0305 mL	5.0763 mL
	40 mM	0.1523 mL	0.7615 mL	1.5229 mL	3.8073 mL
	50 mM	0.1218 mL	0.6092 mL	1.2183 mL	3.0458 mL
	60 mM	0.1015 mL	0.5076 mL	1.0153 mL	2.5382 mL
	80 mM	0.0761 mL	0.3807 mL	0.7615 mL	1.9036 mL
	100 mM	0.0609 mL	0.3046 mL	0.6092 mL	1.5229 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

p-Coumaric acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

p-Coumaric acid501-98-4trans-4-Hydroxycinnamic acidEndogenous MetaboliteBacterialApoptosisAnti-inflammtoryA375B16melanomaPreneoplastic lesionsNephrotoxicityInhibitorinhibitorinhibit

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p-Coumaric acid (Synonyms: trans-4-Hydroxycinnamic acid)

Customer Review

Other Forms of p-Coumaric acid:

p-Coumaric acid Related Antibodies

3 Publications Citing Use of MCE p-Coumaric acid

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•Related Screening Libraries:

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Biological Activity

Purity & Documentation

References

Customer Review

p-Coumaric acid Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

p-Coumaric acid Related Classifications

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