2. Epigenetics
  3. Histone Demethylase
  4. PBIT

PBIT 

Cat. No.: HY-101451 Purity: 99.57%
COA Handling Instructions

PBIT est un inhibiteur spécifique des enzymes du domaine interactif 1 riche en Jumonji AT (JARID1). PBIT inhibe JARID1B (KDM5B ou PLU1) histone déméthylase avec une 50 de environ 3 μM. PBIT inhibe également les JARID1A et JARID1C avec des IC50s de 6 μM et 4,9 μM, respectivement.

PBIT is a specific inhibitor of the Jumonji AT-rich Interactive Domain 1 (JARID1) enzymes. PBIT inhibits JARID1B (KDM5B or PLU1) histone demethylase with an IC50 of about 3 μM . PBIT also inhibits JARID1A and JARID1C with IC50s of 6 μM and 4.9 μM, respectively.

For research use only. We do not sell to patients.

PBIT Chemical Structure

PBIT Chemical Structure

CAS No. : 2514-30-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 110 In-stock
Solution
10 mM * 1 mL in DMSO USD 110 In-stock
Solid
5 mg USD 100 In-stock
10 mg USD 160 In-stock
25 mg USD 350 In-stock
50 mg USD 580 In-stock
100 mg USD 900 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

PBIT Related Antibodies

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  • Purity & Documentation

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Description

PBIT is a specific inhibitor of the Jumonji AT-rich Interactive Domain 1 (JARID1) enzymes. PBIT inhibits JARID1B (KDM5B or PLU1) histone demethylase with an IC50 of about 3 μM . PBIT also inhibits JARID1A and JARID1C with IC50s of 6 μM and 4.9 μM, respectively[1].

IC50 & Target

IC50: 3 μM (JARID1B), 6 μM (JARID1A), 4.9 μM (JARID1C)[1]
In Vitro

PBIT inhibits proliferation of cells expressing higher levels of JARID1B. PBIT (1-10 μM for UACC-812 cells, 2.5-10μM for MCF7 and MCF10A cells; 72 hours) inhibits cell proliferation in a JARID1B level-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PBIT Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Human breast cancer cell lines (UACC-812 and MCF7) and human mammary epithelial cells (MCF10A)
Concentration: 1, 3, and 10 μM for UACC-812 cells; 2.5, 5, and 10 μM for MCF7 and MCF10A cells
Incubation Time: 72 hours
Result: Inhibited cell proliferation in a JARID1B level-dependent manner. 10 μM killed most of the UACC-812 cells, but showed minimal toxicity to MCF7 cells and MCF10A cells.
Molecular Weight

241.31

Appearance

Solid

Formula

C14H11NOS
CAS No.
SMILES

O=C1N(C2=CC=C(C)C=C2)SC3=C1C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (207.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1440 mL 20.7202 mL 41.4405 mL
5 mM 0.8288 mL 4.1440 mL 8.2881 mL
10 mM 0.4144 mL 2.0720 mL 4.1440 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (10.36 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.57%

COA (247 KB) HNMR (240 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References
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PBIT Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

PBIT2514-30-9Histone DemethylaseInhibitorinhibitorinhibit

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