1. Epigenetics
  2. Histone Demethylase
  3. PBIT

PBIT 

Cat. No.: HY-101451 Purity: >98.0%
Handling Instructions

PBIT is a specific inhibitor of the Jumonji AT-rich Interactive Domain 1 (JARID1) enzymes. PBIT inhibits JARID1B (KDM5B or PLU1) histone demethylase with an IC50 of about 3 μM . PBIT also inhibits JARID1A and JARID1C with IC50 s of 6 and 4.9 μM, respectively.

For research use only. We do not sell to patients.

PBIT Chemical Structure

PBIT Chemical Structure

CAS No. : 2514-30-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 220 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

PBIT is a specific inhibitor of the Jumonji AT-rich Interactive Domain 1 (JARID1) enzymes. PBIT inhibits JARID1B (KDM5B or PLU1) histone demethylase with an IC50 of about 3 μM . PBIT also inhibits JARID1A and JARID1C with IC50 s of 6 and 4.9 μM, respectively[1].

IC50 & Target

IC50: 3 μM (JARID1B)[1]

In Vitro

PBIT inhibits proliferation of cells expressing higher levels of JARID1B. PBIT (1-10 μM for UACC-812 cells, 2.5-10μM for MCF7 and MCF10A cells; 72 hours) inhibits cell proliferation in a JARID1B level-dependent manner[1].

Cell Proliferation Assay[1]

Cell Line: Human breast cancer cell lines (UACC-812 and MCF7) and human mammary epithelial cells (MCF10A)
Concentration: 1, 3, and 10 μM for UACC-812 cells; 2.5, 5, and 10 μM for MCF7 and MCF10A cells
Incubation Time: 72 hours
Result: Inhibited cell proliferation in a JARID1B level-dependent manner. 10 μM killed most of the UACC-812 cells, but showed minimal toxicity to MCF7 cells and MCF10A cells.
Molecular Weight

241.31

Formula

C₁₄H₁₁NOS

CAS No.

2514-30-9

SMILES

O=C1N(C2=CC=C(C)C=C2)SC3=C1C=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
References

Purity: >98.0%

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KeyWords:

PBIT | Histone Demethylase | Inhibitor | inhibitor | inhibit

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PBIT
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