Ivosidenib (Synonyms: AG-120)

Name: Ivosidenib
Brand: MedChemExpress
SKU: HY-18767
Rating: 5.0 (19 reviews)

Cat. No.: HY-18767 Purity: 99.89%: Data Sheet
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Ivosidenib (AG-120) is an orally active inhibitor of isocitrate dehydrogenase 1 mutant (mIDH1) enzyme, it exhibits profound d-2-hydroxyglutatrate (2-HG) lowering in vivo. Ivosidenib (AG-120) has the potential for AML therapy due to its acceptable safety profile and clinical activity.

For research use only. We do not sell to patients.

CAS No. : 1448347-49-6

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Customer Review

Based on 19 publication(s) in Google Scholar

Other Forms of Ivosidenib:

(R,S)-Ivosidenib In-stock
IDH1 Inhibitor 8 In-stock

19 Publications Citing Use of MCE Ivosidenib

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Cell Imaging/Staining

: Ivosidenib purchased from MedChemExpress. Usage Cited in: Cancer Drug Resist. 2025 Apr 24:8:20. [Abstract]; Dose-response curves of SKOV3, CAOV3, and OVCAR4 detected by MTT assay treated with cisplatin and 0 μM, 1 μM, 5 μM, 10 μM, 20 μM, and 50 μM Ivosidenib for 72 h.

: Ivosidenib purchased from MedChemExpress. Usage Cited in: Cancer Drug Resist. 2025 Apr 24:8:20. [Abstract]; Quantification of cell colony number in SKOV3, CAOV3, and OVCAR4 cells treated with Ivosidenib (10 μM), Cisplatin, or their combination.

: Ivosidenib purchased from MedChemExpress. Usage Cited in: J Med Chem. 2023 Apr 13;66(7):5279-5288. [Abstract]; Ivosidenib is the most potent IDH1 R132H inhibitor, exhibiting similar binding affinities to wild-type IDH1 and IDH1 R132H, with KD values of 416 nM and 488 nM, respectively.

: Ivosidenib purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Aug 15;13(1):4785. [Abstract]; Inhibition (%) of IDH1 variants (400 nM) by Ivosidenib (10 µM) as determined by the NADPH absorbance assay. Errors: standard errors of the mean (n = 3 independent replicates measured on the same 96-well plate).

: Ivosidenib purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Aug 15;13(1):4785. [Abstract]; Non-denaturing MS analysis measuring binding of Ivosidenib to IDH1 variants. At 20 µM IDH1 variants are predominantly dimeric with 2 NADP(H) molecules bound-dashed line. Green background corresponds to binding of one Ivosidenib molecule. Final concentrations of Ivosidenib: 5 µM (4:1), 20 µM (1:1), and 160 µM (1:8). Cone-voltage: 100 V.

: Ivosidenib purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Aug 15;13(1):4785. [Abstract]; LN18 cells were treated with the inhibitors Ivosidenib (24 h), GSK864, FT-2102, IDH224, and DS-1001B in DMSO (final concentration: 5 µM). 2-HG levels were determined by anion-exchange chromatography coupled to MS51 (errors: standard errors of the mean, n = 4 independent replicates).

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IDH1 IDH2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IDH1

Cellular Effect

Cell Line	Type	Value	Description	References
HT-1080	IC₅₀	8 nM Compound: 99; AG-120	Antiproliferative activity against human HT-1080 cells assessed as cell growth inhibition incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human HT-1080 cells assessed as cell growth inhibition incubated for 48 hrs by sulforhodamine B assay	[PMID: 37235998]
HT-1080	IC₅₀	8 nM Compound: 99; AG-120	Antiproliferative activity against human HT-1080 cells harboring IDH1 R132H mutant measured after 72 hrs by MTT assay Antiproliferative activity against human HT-1080 cells harboring IDH1 R132H mutant measured after 72 hrs by MTT assay	[PMID: 37235998]
U-87MG ATCC	IC₅₀	0.56 μM Compound: 2; AG-120	Synergistic antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 48 hrs in presence of FK866 by CCK8 assay Synergistic antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 48 hrs in presence of FK866 by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	0.98 μM Compound: 2; AG-120	Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	1.02 μM Compound: 2; AG-120	Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	100.06 μM Compound: 2; AG-120	Antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 24 hrs by CCK8 assay Antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 24 hrs by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	19 nM Compound: 99; AG-120	Antiproliferative activity against human U-87 MG cells assessed as reduction in cell growth by CCK-8 assay Antiproliferative activity against human U-87 MG cells assessed as reduction in cell growth by CCK-8 assay	[PMID: 37235998]
U-87MG ATCC	IC₅₀	19 nM Compound: 99; AG-120	Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant measured after 72 hrs by MTT assay Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant measured after 72 hrs by MTT assay	[PMID: 37235998]
U-87MG ATCC	IC₅₀	2.51 μM Compound: 2; AG-120	Synergistic antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 48 hrs in presence of FK866 by CCK8 assay Synergistic antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 48 hrs in presence of FK866 by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	3.06 μM Compound: 2; AG-120	Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 24 hrs by CCK8 assay Antiproliferative activity against human U-87 MG cells harboring IDH1 R132H mutant assessed as inhibition of cell growth incubated for 24 hrs by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	67.22 μM Compound: 2; AG-120	Antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 38651495]
U-87MG ATCC	IC₅₀	89.34 μM Compound: 2; AG-120	Antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay Antiproliferative activity against human U-87 MG cells harboring wildtype IDH1 assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay	[PMID: 38651495]

In Vitro

Ivosidenib (AG-120) (0-13 μM; 48 hours) inhibits several IDH1-R132 mutants with potency similar IC₅₀ values: IDH1-R132H (IC₅₀=12 nM); IDH1-R132C (IC₅₀=13 nM); IDH1-R132G (IC₅₀=8 nM); IDH1-R132L (IC₅₀=13 nM); IDH1-R132S (IC₅₀=12 nM), respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AG-120 (gavage administration; 50 mg/kg and 150 mg/kg) declines tumor 2-HG concentration rapidly, with maximum inhibition (92.0% and 95.2% at the 50 mg/kg and 150 mg/kg doses, respectively) achieved at -12 h post dose^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female nude BALB/c mice inoculated with HT1080 cells^[1]
Dosage:	50 mg/kg and 150 mg/kg
Administration:	Gavage administration; 50 mg/kg and 150 mg/kg
Result:	Showed robust tumor 2-HG reduction in mouse.

Clinical Trial

Molecular Weight

582.96

Formula

C₂₈H₂₂ClF₃N₆O₃

CAS No.

1448347-49-6

Appearance

Solid

Color

White to off-white

SMILES

ClC1=C([C@H](N(C2=CC(F)=CN=C2)C([C@H]3N(C4=NC=CC(C#N)=C4)C(CC3)=O)=O)C(NC5CC(F)(F)C5)=O)C=CC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (171.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7154 mL	8.5769 mL	17.1538 mL
5 mM	0.3431 mL	1.7154 mL	3.4308 mL
10 mM	0.1715 mL	0.8577 mL	1.7154 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (8.58 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (258 KB) HNMR (138 KB) RP-HPLC (225 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Popovici-Muller J, et al. Discovery of AG-120 (Ivosidenib): A First-in-Class Mutant IDH1 Inhibitor for the Treatment of IDH1Mutant Cancers. ACS Med Chem Lett. 2018 Jan 19;9(4):300-305. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7154 mL	8.5769 mL	17.1538 mL	42.8846 mL
	5 mM	0.3431 mL	1.7154 mL	3.4308 mL	8.5769 mL
	10 mM	0.1715 mL	0.8577 mL	1.7154 mL	4.2885 mL
	15 mM	0.1144 mL	0.5718 mL	1.1436 mL	2.8590 mL
	20 mM	0.0858 mL	0.4288 mL	0.8577 mL	2.1442 mL
	25 mM	0.0686 mL	0.3431 mL	0.6862 mL	1.7154 mL
	30 mM	0.0572 mL	0.2859 mL	0.5718 mL	1.4295 mL
	40 mM	0.0429 mL	0.2144 mL	0.4288 mL	1.0721 mL
	50 mM	0.0343 mL	0.1715 mL	0.3431 mL	0.8577 mL
	60 mM	0.0286 mL	0.1429 mL	0.2859 mL	0.7147 mL
	80 mM	0.0214 mL	0.1072 mL	0.2144 mL	0.5361 mL
	100 mM	0.0172 mL	0.0858 mL	0.1715 mL	0.4288 mL

Ivosidenib Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ivosidenib1448347-49-6AG-120AG120AG 120Isocitrate Dehydrogenase (IDH)Inhibitorinhibitorinhibit

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Ivosidenib (Synonyms: AG-120)

Customer Review

Other Forms of Ivosidenib:

Ivosidenib Related Antibodies

19 Publications Citing Use of MCE Ivosidenib

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Isocitrate Dehydrogenase (IDH) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Ivosidenib Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ivosidenib Related Classifications

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