PBRM1-BD2-IN-4
PBRM1-BD2-IN-4 is a PBRM1-BD2 inhibitor with an IC50 of 0.2 μM, and its Kd values for PBRM1-BD2 and PBRM1-BD5 are 5.5 μM and 11.1 μM, respectively. PBRM1-BD2-IN-4 selectively inhibits the proliferation of PBRM1-dependent prostate cancer cells. PBRM1-BD2-IN-4 can be used in studies related to prostate cancer.
For research use only. We do not sell to patients.
- CAS No.: 2819989-60-9
- Formula: C15H13ClN2O
- Molecular Weight:272.73
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[1]|
BD2 0.2 μM (IC50) |
In Vitro
PBRM1-BD2-IN-4 (Compound 12) (0.1-10 μM; 5 days) inhibits the proliferation of LNCaP cells in a dose-dependent manner, while exerts no significant effect on PC3 and PWPE-1 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:LNCaP, PC3 and PWPE-1 cell
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Concentration:0.1, 1 and 10 μM
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Incubation Time:5 days
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Result:Inhibited the proliferation of LNCaP cells in a dose-dependent manner, while exerts no significant effect on PC3 and PWPE-1 cells.
Chemical Information
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CAS No. 2819989-60-9
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Molecular Weight 272.73
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Formula C15H13ClN2O
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SMILES
O=C1NC(C2=CC=CC=C2C)NC3=C1C(Cl)=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PBRM1-BD2-IN-4 Related Classifications
HY-151531 Related Classifications
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