Leuhistin, a new inhibitor of aminopeptidase M, produced by Bacillus laterosporus BMI156-14F1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities

  • J Antibiot (Tokyo). 1991 Jun;44(6):573-8. doi: 10.7164/antibiotics.44.573.
T Aoyagi  1 ,  S Yoshida ,  N Matsuda ,  T Ikeda ,  M Hamada ,  T Takeuchi
Affiliations
  • 1. Institute of Microbial Chemistry, Tokyo, Japan.
PMID: 1676997 DOI: 10.7164/antibiotics.44.573
Abstract

Leuhistin has been isolated from the culture broth of Bacillus laterosporus BMI156-14F1 as part of a program designed to find microorganism-produced inhibitors of Aminopeptidase M (AP-M). It was purified by use of column chromatography on Sepabeads SP206, Amberlite IRC-50, MCI gel CHP-20P and Sephadex G-10 and then isolated as colorless needles. Leuhistin inhibits AP-M strongly and it also inhibits AP-A and AP-B weakly. It is competitive with the substrate, and the inhibition constant (Ki) was 2.3 x 10(-7) M.

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