In Vivo Dual-Modality Fluorescence and Magnetic Resonance Imaging-Guided Lymph Node Mapping with Good Biocompatibility Manganese Oxide Nanoparticles

  • Molecules. 2017 Dec 12;22(12):2208. doi: 10.3390/molecules22122208.
Yonghua Zhan  1  2 ,  Wenhua Zhan  3  4 ,  Hanrui Li  5 ,  Xinyi Xu  6 ,  Xu Cao  7 ,  Shouping Zhu  8 ,  Jimin Liang  9 ,  Xueli Chen  10
Affiliations
  • 1. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 2. Xidian-Ningbo Information Technology Institute, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 3. Department of Radiotherapy, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China. [email protected].
  • 4. Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China. [email protected].
  • 5. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 6. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 7. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 8. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 9. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
  • 10. Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China. [email protected].
PMID: 29231865 DOI: 10.3390/molecules22122208
Abstract

Multifunctional manganese oxide nanoparticles (NPs) with impressive enhanced T₁ contrast ability show great promise in biomedical diagnosis. Herein, we developed a dual-modality imaging agent system based on polyethylene glycol (PEG)-coated manganese oxide NPs conjugated with organic dye (Cy7.5), which functions as a fluorescence imaging (FI) agent as well as a magnetic resonance imaging (MRI) imaging agent. The formed Mn₃O₄@PEG-Cy7.5 NPs with the size of ~10 nm exhibit good colloidal stability in different physiological media. Serial FI and MRI studies that non-invasively assessed the bio-distribution pattern and the feasibility for in vivo dual-modality imaging-guided lymph node mapping have been investigated. In addition, histological and biochemical analyses exhibited low toxicity even at a dose of 20 mg/kg in vivo. Since Mn₃O₄@PEG-Cy7.5 NPs exhibited desirable properties as imaging agents and good biocompatibility, this work offers a robust, safe, and accurate diagnostic platform based on manganese oxide NPs for tumor metastasis diagnosis.

Keywords
dual-modality; fluorescence imaging; lymph node; magnetic resonance imaging; manganese oxide nanoparticles.
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