Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection

  • ACS Appl Mater Interfaces. 2019 Mar 20;11(11):10554-10558. doi: 10.1021/acsami.9b00010.
Shanshan Jiang  1 ,  Yifan Zhang  1 ,  Yichen Yang  1 ,  Yan Huang  1 ,  Gongcheng Ma  1 ,  Yongxiang Luo  1 ,  Peng Huang  1 ,  Jing Lin  1
Affiliations
  • 1. Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, International Cancer Center, Laboratory of Evolutionary Theranostics, School of Biomedical Engineering , Shenzhen University Health Science Center , Shenzhen 518060 , China.
PMID: 30807088 DOI: 10.1021/acsami.9b00010
Abstract

The accurate detection of glucose at cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx), and silver ion fluorescence probe (denoted as AgNC-GOx/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that AgNC-GOx/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterward, the feasibility of using AgNC-GOx/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.

Keywords
fluorescence probe; glucose oxidase; intracellular glucose; signal amplification; silver nanocube.
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