TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers

  • J Cancer. 2020 Aug 25;11(20):6122-6132. doi: 10.7150/jca.47290.
Jiaqi Dai  1 ,  Xiaqing Yu  2 ,  Yali Han  1 ,  Li Chai  2 ,  Yina Liao  1 ,  Peng Zhong  2 ,  Ruting Xie  3 ,  Xuechen Sun  3 ,  Qingqing Huang  2 ,  Jian Wang  4 ,  Zhiqiang Yin  1 ,  Yun Zhang  1 ,  Zhongwei Lv  1  2 ,  Chengyou Jia  1
Affiliations
  • 1. Shanghai Research Center for Thyroid Diseases, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, P. R. China.
  • 2. Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, P. R. China.
  • 3. Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, P. R. China.
  • 4. Department of Nuclear Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, P. R. China.
PMID: 32922552 DOI: 10.7150/jca.47290
Abstract

Background and Aim: Invasion and metastasis are critical events in papillary thyroid carcinoma (PTC) progression. Protein markers specific to this process may avoid over-treatment and urgently needed. Methods: TMT-labeled mass spectrometry-based proteomics were carried out on PTC and invasive phenotype (iPTC) (3 pairs per group) and cross validate differentially expressed proteins (DEPs) (FC>1.5 and <0.67 and p<0.05) with GEO and TCGA datasets and the correlation genes of DEPs were also analyzed. Results: We identified and quantified 4607 proteins identical to PTC and iPTC groups. Among which 12 DEPs in PTC and 179 DEPs in iPTCs were found. Cross-validation with GSE60542 and TCGA database revealed 10 DEPs that all significant correlated with metastasis and staging. Upregulated SLC27A6 showed negative correlation with 6 out of 9 downregulated DEPs including HGD, CA4, COL23A1, SLC26A7, FHL1 and TPO. Conclusion: The panel of 7 genes (SLC27A6 and 6 downregulated DEPs) could have ideal prediction value to improve our understanding of invasiveness of PTC.

Keywords
differentially expressed proteins; invasiveness; markers; papillary thyroid carcinoma; tandem-mass tag.