Molecular cloning and functional characterization of a human VIP receptor from SUP-T1 lymphoblasts

  • Biochem Biophys Res Commun. 1994 Dec 30;205(3):1617-24. doi: 10.1006/bbrc.1994.2852.
M Svoboda  1 ,  M Tastenoy ,  J Van Rampelbergh ,  J F Goossens ,  P De Neef ,  M Waelbroeck ,  P Robberecht
Affiliations
  • 1. Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
PMID: 7811244 DOI: 10.1006/bbrc.1994.2852
Abstract

We have cloned and sequenced a cDNA isolated from a human SUP-T1 lymphoblast cell line library. It encoded a 457 Amino acids protein having 87% identity with the rat PACAP type II, VIP2 receptor. Chinese hamster ovary (CHO) cells stably transfected with cloned cDNA expressed a specific binding of 125I[Acetyl-His1]PACAP-27. This binding was inhibited by GTP, and by the Peptides helodermin, VIP, PACAP-27 and PACAP-38 that also stimulated Adenylate Cyclase activity. The order of potency was PACAP-38 > VIP > or = helodermin > or = PACAP-27. Comparison of the results in two cell lines expressing different receptor densities suggested that helodermin and PACAP-38 had a higher intrinsic activity than VIP and PACAP-27.