2. JAK/STAT Signaling Stem Cell/Wnt Autophagy Epigenetics Protein Tyrosine Kinase/RTK
  3. STAT Autophagy JAK
  4. Pulchinenoside E2

Pulchinenoside E2 is a triterpenoid saponin. Pulchinenoside E2 inhibits the phosphorylation of STAT3 and JAK2, blocks the nuclear translocation and transcriptional activity of STAT3, and suppresses STAT3-dependent mitochondrial oxidative phosphorylation. Pulchinenoside E2 inhibits invasion, migration and autophagy of triple-negative breast cancer cells. Pulchinenoside E2 can be used in research related to triple-negative breast cancer.

For research use only. We do not sell to patients.

Pulchinenoside E2

Pulchinenoside E2 Chemical Structure

CAS No. : 244202-36-6

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Pulchinenoside E2 Related Antibodies

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Description

Pulchinenoside E2 is a triterpenoid saponin. Pulchinenoside E2 inhibits the phosphorylation of STAT3 and JAK2, blocks the nuclear translocation and transcriptional activity of STAT3, and suppresses STAT3-dependent mitochondrial oxidative phosphorylation. Pulchinenoside E2 inhibits invasion, migration and autophagy of triple-negative breast cancer cells. Pulchinenoside E2 can be used in research related to triple-negative breast cancer[1].

IC50 & Target[1]

JAK2

 

STAT3

 

Cellular Effect
Cell Line Type Value Description References
A-375 IC50
5.9 μM
Compound: 9
Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay
Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay
[PMID: 23639650]
A549 IC50
7.9 μM
Compound: 9
Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay
Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay
[PMID: 23639650]
HL-60 IC50
2.6 μg/mL
Compound: 8
Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
[PMID: 10514313]
HL-60 IC50
3.5 μM
Compound: 9
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
[PMID: 23639650]
In Vitro

Pulchinenoside E2 (0.75-1.5 μM; 24 h) induces lysosomal dysfunction in HS-578T and MDA-MB-231 TNBC cells, characterized by suppressed proteolytic activity, time-dependent degradation of mature CTSD, and lysosomal alkalinization[1].
Pulchinenoside E2 (0.75-1.5 μM; 24 h) dose-dependently increases GFP-LC3B puncta formation and impairs autophagic flux in HS-578T and MDA-MB-231 TNBC cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pulchinenoside E2 Related Antibodies

Cell Autophagy Assay[1]

Cell Line: HS-578T, MDA-MB-231 TNBC cells
Concentration: 0.75, 1.5 μM
Incubation Time: 24 h
Result: Dose-dependently increased the number of autophagic puncta and fluorescence intensity of GFP-LC3B in both cell lines.
Dose-dependently increased the LC3II/LC3I ratio and accumulation of the autophagy substrate p62.
Parmacokinetics
Species Dose Route Tmax Cmax T1/2 AUC0-t MRTlast
Mice[1] 5 mg/kg i.p. 6.30 h 35.70 μg/mL >12 h 338.80 μg·h/mL 6.67 h
In Vivo

Pulchinenoside E2 (5-10 mg/kg; i.p.; administered every other day for 30 consecutive days) exhibits significant dose-dependent anti-metastatic activity in a mouse model of triple-negative breast cancer (TNBC)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: M-NSG mice (female, 4-6 weeks old, tail vein injection of MDA-MB-231 cells to induce metastases)[2]
Dosage: 5 mg/kg; 10 mg/kg
Administration: i.p.; alternate days; 30 days
Result: Markedly reduced pulmonary and hepatic metastatic lesion numbers and tumor volumes in mice treated with 10 mg/kg compared to controls.
Significantly decreased phosphorylated STAT3 (Tyr705/Ser727) expression in metastatic tumors via immunohistochemical staining.
Accumulated LC3B and p62 in metastatic tumors, consistent with autophagic flux blockade.
Maintained serum AST, ALT, CRE, and BUN levels comparable to controls.
Showed no significant histopathological damage in heart and kidney tissues.
Maintained stable body weight throughout treatment, indicating no overt toxicity.
Molecular Weight

1059.24

Formula

C53H86O21
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1(C)[C@@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](C)O3)[C@@H](O)[C@@H](O)CO2)CC[C@@]6(C)[C@@]1([H])CC[C@]7(C)[C@]6([H])CC=C8[C@@]7(C)CC[C@]9(C(O)=O)[C@@]8([H])CC(C)(C)CC9
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Purity & Documentation
References
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Pulchinenoside E2 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pulchinenoside E2244202-36-6Pulchinenoside E 2Pulchinenoside E-2STATAutophagyJAKJanus kinasenuclear translocationJAK2autophagyMDA-MB-231HS-578Tmitochondrial oxidative phosphorylationmouse modelstriple-negative breast cancer cellslysosomal proteolytic activitySTAT3Inhibitorinhibitorinhibit

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