Cat. No.: HY-N8098

Pulchinenoside E2

Customer Review

Based on 1 Customer Validation

Pulchinenoside E2 is a triterpenoid saponin. Pulchinenoside E2 inhibits the phosphorylation of STAT3 and JAK2, blocks the nuclear translocation and transcriptional activity of STAT3, and suppresses STAT3-dependent mitochondrial oxidative phosphorylation. Pulchinenoside E2 inhibits invasion, migration and autophagy of triple-negative breast cancer cells. Pulchinenoside E2 can be used in research related to triple-negative breast cancer.

For research use only. We do not sell to patients.

Purity: 99.28%
CAS No.: 244202-36-6
Formula: C₅₃H₈₆O₂₁
Molecular Weight:1059.24

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Pulchinenoside E2 Chemical Structure

CAS No.: 244202-36-6

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Parmacokinetics
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

JAK2

STAT3

All STAT Isoforms :

All JAK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	5.9 μM Compound: 9	Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay	[PMID: 23639650]
A549	IC₅₀	7.9 μM Compound: 9	Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 23639650]
HL-60	IC₅₀	2.6 μg/mL Compound: 8	Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay	[PMID: 10514313]
HL-60	IC₅₀	3.5 μM Compound: 9	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 23639650]

In Vitro

Pulchinenoside E2 (0.75-1.5 μM; 24 h) induces lysosomal dysfunction in HS-578T and MDA-MB-231 TNBC cells, characterized by suppressed proteolytic activity, time-dependent degradation of mature CTSD, and lysosomal alkalinization^[1].
Pulchinenoside E2 (0.75-1.5 μM; 24 h) dose-dependently increases GFP-LC3B puncta formation and impairs autophagic flux in HS-578T and MDA-MB-231 TNBC cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Autophagy Assay^[1]

Cell Line:

HS-578T, MDA-MB-231 TNBC cells
Concentration:

0.75, 1.5 μM
Incubation Time:

24 h
Result:

Dose-dependently increased the number of autophagic puncta and fluorescence intensity of GFP-LC3B in both cell lines.
Dose-dependently increased the LC3II/LC3I ratio and accumulation of the autophagy substrate p62.

Parmacokinetics

Species	Dose	Route	T_max	C_max	T_1/2	AUC_0-t	MRT_last
Mice^[1]	5 mg/kg	i.p.	6.30 h	35.70 μg/mL	>12 h	338.80 μg·h/mL	6.67 h

In Vivo

Pulchinenoside E2 (5-10 mg/kg; i.p.; administered every other day for 30 consecutive days) exhibits significant dose-dependent anti-metastatic activity in a mouse model of triple-negative breast cancer (TNBC)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

M-NSG mice (female, 4-6 weeks old, tail vein injection of MDA-MB-231 cells to induce metastases)^[2]
Dosage:

5 mg/kg; 10 mg/kg
Administration:

i.p.; alternate days; 30 days
Result:

Markedly reduced pulmonary and hepatic metastatic lesion numbers and tumor volumes in mice treated with 10 mg/kg compared to controls.
Significantly decreased phosphorylated STAT3 (Tyr705/Ser727) expression in metastatic tumors via immunohistochemical staining.
Accumulated LC3B and p62 in metastatic tumors, consistent with autophagic flux blockade.
Maintained serum AST, ALT, CRE, and BUN levels comparable to controls.
Showed no significant histopathological damage in heart and kidney tissues.
Maintained stable body weight throughout treatment, indicating no overt toxicity.

Chemical Information

CAS No. 244202-36-6
Appearance Solid
Molecular Weight 1059.24
Formula C₅₃H₈₆O₂₁
Color White to off-white
SMILES

CC1(C)[C@@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](C)O3)[C@@H](O)[C@@H](O)CO2)CC[C@@]6(C)[C@@]1([H])CC[C@]7(C)[C@]6([H])CC=C8[C@@]7(C)CC[C@]9(C(O)=O)[C@@]8([H])CC(C)(C)CC9
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zheng D, et al. Pulchinenoside E2: A dual-functional STAT3 and autophagy inhibitor with potent anti-metastatic activity in triple-negative breast cancer. Phytomedicine. 2025;148:157485. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Pulchinenoside E2

Epigenetics Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Small Molecule Immuno-Oncology Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Glycoside Compound Library

Terpenoids Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Autoimmune Disease Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

All STAT Isoforms

All JAK Isoforms