1. Cell Cycle/DNA Damage
    Anti-infection
  2. Antifolate
    Parasite
  3. Pyrimethamine

Pyrimethamine (Synonyms: Pirimecidan; Pirimetamin; RP 4753)

Cat. No.: HY-18062 Purity: 99.94%
Handling Instructions

Pyrimethamine (Pirimecidan) is a potent, orally active dihydrofolate reductase (DHFR) inhibitor. Pyrimethamine is an antimalarial agent. Pyrimethamine affects the nucleoprotein metabolism of malarial parasites by interference in the folic–folinic acid systems and affects cell division by inhibiting the conversion of dihydrofolate to tetrahydrofolate.

For research use only. We do not sell to patients.

Pyrimethamine Chemical Structure

Pyrimethamine Chemical Structure

CAS No. : 58-14-0

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Solution
10 mM * 1 mL in DMSO USD 87 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 87 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 79 In-stock
Estimated Time of Arrival: December 31
500 mg USD 185 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Pyrimethamine:

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  • Biological Activity

  • Purity & Documentation

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Description

Pyrimethamine (Pirimecidan) is a potent, orally active dihydrofolate reductase (DHFR) inhibitor. Pyrimethamine is an antimalarial agent. Pyrimethamine affects the nucleoprotein metabolism of malarial parasites by interference in the folic–folinic acid systems and affects cell division by inhibiting the conversion of dihydrofolate to tetrahydrofolate[1][2].

In Vitro

Pyrimethamine (Pirimecidan; 4 nM-4 μM; 24 h; LLC-MK2 cells with T. gondii) combination of Fluconazole (FLZ) (HY-B0101) inhibits T. gondii activity with IC50 values of 0.23, 0.19, 0.23, 0.34, 0.14, and 0.19 μM for FLZ concentration at 0, 0.05, 0.1, 0.5, 1.0, and 3.0 μM, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: LLC-MK2 cells with T. gondii
Concentration: 4 nM-4 μM
Incubation Time: 24 hours
Result: Inhibited T. gondii activity and decreased parasite proliferation index.
In Vivo

Pyrimethamine (Pirimecidan; 1 mg/kg; i.g.; daily, for 10 d; female CF1 mice with T. gondii xenograft) combination of Fluconazole (FLZ) (HY-B0101) and Sulfadiazine (HY-B0273) increases protection from death[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female CF1 mice (18-22 g; 4-6 week of age) with T. gondii xenograft[1]
Dosage: Oral gavage; daily, for 10 days
Administration: 1 mg/kg; 10 mg/kg (Fluconazole (HY-B0101)), 40 mg/kg (Sulfadiazine (HY-B0273))
Result: Increased mouse survival compared to treatment with SDZ/PYR alone.
Clinical Trial
Molecular Weight

248.71

Formula

C12H13ClN4

CAS No.
SMILES

NC1=NC(N)=C(C2=CC=C(Cl)C=C2)C(CC)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (80.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0207 mL 20.1037 mL 40.2075 mL
5 mM 0.8041 mL 4.0207 mL 8.0415 mL
10 mM 0.4021 mL 2.0104 mL 4.0207 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.05 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.05 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.94%

References
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Product Name:
Pyrimethamine
Cat. No.:
HY-18062
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