1 Result for "

5-VIC Phosphoramidite

" in MedChemExpress (MCE) Product Catalog:
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1 Results for "5-VIC Phosphoramidite" in MCE Product Catalog:

Cat. No.: HY-W1061847
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5-VIC Phosphoramidite is a Fluorescent dye and a component of TaqMan probes for nucleic acid detection. 5-VIC Phosphoramidite serves as a 5' reporter dye, which is conjugated to probes targeting the conserved region of the S protein of porcine epidemic diarrhea virus genotype GII, and is used with Eclipse® Dark Quencher for duplex real-time quantitative PCR. 5-VIC Phosphoramidite-labeled probes targeting the Rv0222 gene in the RD4 region specific to Mycobacterium tuberculosis are applied in triplex droplet digital PCR to differentiate mycobacterial strains. During PCR amplification, the 5'→3' exonuclease activity of Taq polymerase mediates the separation of 5-VIC Phosphoramidite from the quencher, thereby generating a detectable fluorescent signal .
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