32 Results for "

nucleic acid analysis

" in MedChemExpress (MCE) Product Catalog:
Products (32)

32 Results for "nucleic acid analysis" in MCE Product Catalog:

5
5 Cited Publications
Cat. No.: HY-D1191
CAS No.: 2225748-05-8
SYBR Green I chloride is a highly sensitive fluorescent nucleic acid dye that binds specifically to the minor groove of double-stranded DNA or intercalates between base pairs. SYBR Green I chloride exhibits weak fluorescence in the unbound state but emits bright fluorescence upon binding, and it preferentially binds to large-fragment DNA and DNA with high G+C content. SYBR Green I chloride is suitable for real-time PCR technology; its fluorescence intensity correlates with the amount and size of amplification products, enabling accurate quantification of gene expression and discrimination of amplicons via melting curve analysis without additional post-processing. SYBR Green I chloride is widely used in preclinical in vitro nucleic acid detection .
loading...
    loading...
4
4 Cited Publications
Cat. No.: HY-D0971
CAS No.: 92-32-0
Synonyms: Pyronine G; C.I. 45005
Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells .
loading...
    loading...
1
1 Cited Publications
Cat. No.: HY-D0150
CAS No.: 107091-89-4
Thiazole Orange is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. Thiazole Orange has been widely used in biomolecular detection and staining of DNA/ RNA in gels and can be used for reticulocyte analysis. Thiazole orange generates a significant fluorescence enhancement and high quantum yield when it binds with nucleic acids, especially RNA. Thiazole orange can permeate living cell membranes. Thiazole orange can use UV light for detection, but can also be detected with blue light. The excitation and emission of Thiazole orange are λex = 510 nm (488 nm and 470 nm also show strong excitation) and λem = 527 nm, respectively .
loading...
    loading...
1
1 Cited Publications
Cat. No.: HY-15906
CAS No.: 122341-56-4
Purity:  99.93%
Synonyms: Lumi-Phos Plus; Lumigen PPD; PPD
AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields .
loading...
    loading...
1
1 Cited Publications
Cat. No.: HY-W013159
CAS No.: 33430-61-4
Synonyms: 5′-dGMP disodium
2'-Deoxyguanosine 5'-monophosphate (5′-dGMP) disodium is a mononucleotide having guanine as the nucleobase. 2'-Deoxyguanosine 5'-monophosphate disodium is a reactant involved in analysis of self-assembling in solution and nucleation/growth of G-qudruplexes, nucleophilic trapping and reductive alkylation. 2'-Deoxyguanosine 5'-monophosphate disodium can be used as an oxidizable target. 2'-Deoxyguanosine 5'-monophosphate disodium is a nucleic acid guanosine triphosphate (GTP) derivative and is a nucleotide precursor used in DNA synthesis .
loading...
    loading...
Cat. No.: HY-D0860
CAS No.: 5704-04-1
Tricine is widely used as a buffer in protein electrophoresis, nucleic acid analysis, and immunology, which can help adjust pH and stabilize chemical reactions. In addition, the compound can also be used in research in certain medical fields, such as in antibody detection and drug screening.
loading...
    loading...
Cat. No.: HY-E70529
Research Areas:  

Infection

Ribonucleoside vanadyl complexes are a class of potent RNase and Taq polymerase inhibitors. Ribonucleoside vanadyl complexes protect RNA during RNA isolation by inhibiting ribonucleases, and also reduce the viability of bacteria and eukaryotic cells by interfering with ribosomal subunit assembly. Ribonucleoside vanadyl complexes block PCR and reverse transcription reactions templated by viral nucleic acids and enhance the effects of antibiotics against Staphylococcus aureus, but do not directly inhibit protein synthesis. Ribonucleoside vanadyl complexes can be effectively removed by phenol-chloroform extraction, thus enabling subsequent PCR analysis. Ribonucleoside vanadyl complexes can be applied in research related to chronic hepatitis C (HCV) and Staphylococcus aureus infection .
loading...
    loading...
Cat. No.: HY-169798
CAS No.: 23481-50-7
Target:  

Fluorescent Dye VSV

Research Areas:  

Infection

1,9-Dimethylmethylene blue is a photosensitizer, virus inactivator and hemoglobin oxidant derived from methylene blue. When activated, 1,9-Dimethylmethylene blue generates reactive oxygen species including singlet oxygen, and acts as a metachromatic dye. When activated in monomeric or dimeric form, 1,9-Dimethylmethylene blue induces photoinactivation of R17 phage and vesicular stomatitis virus and oxidizes hemoglobin via non-singlet oxygen reactive oxygen species or singlet oxygen-mediated pathways, respectively. The monomeric form, with higher nucleic acid affinity, achieves virus inactivation under specific conditions without forming methemoglobin. 1,9-Dimethylmethylene blue binds to substances such as glycosaminoglycans to produce color changes. Although it is susceptible to interference from non-glycosaminoglycan components in urine, it is still applicable to spectrophotometric analysis for glycosaminoglycan quantification. With these unique photochemical and binding properties, 1,9-Dimethylmethylene blue is widely used in studies of viral infections and related biochemical analyses .
loading...
    loading...
Cat. No.: HY-Y0030A
CAS No.: 874-24-8
Synonyms: 3-HPA
3-Hydroxypicolinic acid, matrix substance for MALDI-MS is a matrix. 3-Hydroxypicolinic acid, matrix substance for MALDI-MS coordinates with metal ions such as copper to form complexes with cytotoxic and antimycobacterial activity. 3-Hydroxypicolinic acid, matrix substance for MALDI-MS is used for the analysis of oligodeoxynucleotides in matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). 3-Hydroxypicolinic acid, matrix substance for MALDI-MS is useful for nucleic acid analysis and anti-tuberculosis compound development .
loading...
    loading...
Cat. No.: HY-132142
CAS No.: 115899-39-3
Purity:  ≥98.0%
5-Propargylamino-dCTP is a sustrate for DNA polymerases. 5-Propargylamino-dCTP can conjugate to molecular markers for use in nucleic acid labeling or sequence analysis .
loading...
    loading...
Cat. No.: HY-W133919
CAS No.: 28631-66-5
Aniline Blue sodium is a water-soluble dye commonly used as a biological stain for the detection of nucleic acids and proteins in various laboratory procedures such as electrophoresis and microscopy. Aniline Blue sodium has unique chemical properties that allow it to bind to specific cellular components, producing a color change that facilitates their visualization and analysis.
loading...
    loading...
Cat. No.: HY-W777012
CAS No.: 177332-37-5
Acridinium C2 NHS ester is a chemiluminescent label that is active for the development of protein and nucleic acid probes. Acridinium C2 NHS ester can be used in bioanalysis and diagnostics to provide highly sensitive detection solutions. Acridinium C2 NHS ester is widely used in medical research, especially in the monitoring and analysis of biomolecules.
loading...
    loading...
Cat. No.: HY-156045C
CAS No.: 9041-36-5
Cross-linked dextran G 200 is a hydrophilic gel based on molecular size exclusion and targeted macromolecular separation. Cross-linked dextran G 200 works through the gel permeation mechanism, and the cross-linked structure forms a three-dimensional network with a specific pore size, achieving separation based on the molecular hydrodynamic volume. Cross-linked dextran G 200 can be used to adjust the osmotic solute distribution coefficient and the ability to maintain osmotic equilibrium, such as in gel filtration chromatography for purification and analysis of biomacromolecules such as proteins and nucleic acids . Cross-linked dextran G 200 can also be used as a gel filtration filler (particle size range: 40-120 μm; globular protein separation range: 5-600 kDa) .
loading...
    loading...
Cat. No.: HY-156045B
CAS No.: 12774-36-6
Cross-linked dextran G 150 is a hydrophilic gel based on molecular size exclusion and targeted for the separation of macromolecules. Cross-linked dextran G 150 functions through the gel permeation mechanism, where the cross-linked structure forms a three-dimensional network with specific pore diameters, achieving separation based on the molecular volumetric flow. Cross-linked dextran G 150 can be used to regulate the distribution coefficient of permeating solutes and maintain the permeation balance, such as in gel filtration chromatography for the purification and analysis of biological macromolecules like proteins and nucleic acids. Cross-linked dextran G 150 can also be used as a gel filtration filler (particle size range: 40-120 μm; spherical protein separation range: 5-300 kDa) .
loading...
    loading...
Cat. No.: HY-W001952
CAS No.: 15231-91-1
6-Bromo-2-naphthol is an RTP (real-time polymerase chain reaction) probe that forms a 1:1 or 2:1 complex with β-cyclodextrin (β-CD). 6-Bromo-2-naphthol is capable of real-time monitoring of PCR reactions and quantification of specific nucleic acid sequences. RTP probes are a class of small DNA or RNA sequences labeled with fluorescent dyes and quencher molecules, which can be widely used in gene expression analysis, SNP genotyping, and pathogen detection. 6-Bromo-2-naphthol embeds into the cyclodextrin cavity through hydrophobic interactions, inhibits the oxygen quenching effect, and emits a phosphorescent signal at room temperature. 6-Bromo-2-naphthol can also be used as an intermediate for the synthesis of antibacterial azo dyes, and its derivatives show antibacterial activity against Staphylococcus aureus, Escherichia coli and other bacteria .
loading...
    loading...
Cat. No.: HY-132146A
Purity:  ≥99.0%
5-Propargylamino-ddCTP (trisodium) solution (25mM), a nucleoside molecule that can be used to synthesis of cyanine dye-nucleotide conjugate which is used in nucleic acid labeling or sequence analysis .
loading...
    loading...
Cat. No.: HY-W013159S
Synonyms: 5′-dGMP-13C10,15N5 disodium
2'-Deoxyguanosine 5'-monophosphate- 13C10, 15N5 (dGMP- 13C10, 15N5) disodium is the 13C and 15N labeled 2'-Deoxyguanosine 5'-monophosphate disodium (HY-W013159). 2'-Deoxyguanosine 5'-monophosphate (5′-dGMP) disodium is a mononucleotide having guanine as the nucleobase. 2'-Deoxyguanosine 5'-monophosphate disodium is a reactant involved in analysis of self-assembling in solution and nucleation/growth of G-qudruplexes, nucleophilic trapping and reductive alkylation. 2'-Deoxyguanosine 5'-monophosphate disodium can be used as an oxidizable target. 2'-Deoxyguanosine 5'-monophosphate disodium is a nucleic acid guanosine triphosphate (GTP) derivative and is a nucleotide precursor used in DNA synthesis .
loading...
    loading...
Cat. No.: HY-132145
CAS No.: 114748-59-3
5-Propargylamino-ddUTP, a nucleoside molecule that can be used to synthesis of cyanine dye-nucleotide conjugate which is used in nucleic acid labeling or sequence analysis .
loading...
    loading...
Cat. No.: HY-132146
CAS No.: 114748-56-0
5-Propargylamino-ddCTP, a nucleoside molecule that can be used to synthesis of cyanine dye-nucleotide conjugate which is used in nucleic acid labeling or sequence analysis .
loading...
    loading...
Cat. No.: HY-171764
CAS No.: 3688-37-7
Target:  

Fluorescent Dye

Research Areas:  

Others

β-Ethoxy-α-ketobutyraldehyde is a chemical probe. β-Ethoxy-α-ketobutyraldehyde specifically modifies the N1 and N2 positions of guanine. β-Ethoxy-α-ketobutyraldehyde is mainly used to study the steric protection of single-stranded/double-stranded regions of nucleic acids and protein binding sites. β-Ethoxy-α-ketobutyraldehyde can be used for nucleic acid structure analysis and footprint studies of protein-nucleic acid complexes .
loading...
    loading...