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  3. Aniline Blue sodium

Aniline Blue sodium is a water-soluble dye commonly used as a biological stain for the detection of nucleic acids and proteins in various laboratory procedures such as electrophoresis and microscopy. Aniline Blue sodium has unique chemical properties that allow it to bind to specific cellular components, producing a color change that facilitates their visualization and analysis.

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Aniline Blue sodium Chemical Structure

Aniline Blue sodium Chemical Structure

CAS No. : 28631-66-5

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Based on 1 publication(s) in Google Scholar

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Description

Aniline Blue sodium is a water-soluble dye commonly used as a biological stain for the detection of nucleic acids and proteins in various laboratory procedures such as electrophoresis and microscopy. Aniline Blue sodium has unique chemical properties that allow it to bind to specific cellular components, producing a color change that facilitates their visualization and analysis.

In Vitro

Aniline Blue, sodium salt is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

737.73

Formula

C32H25N3Na2O9S3

CAS No.
Appearance

Solid

Color

Purple to black

SMILES

CC1=CC(/C(C2=CC=C(NC3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2)=C4C=C/C(C=C\4)=N/C5=CC=C(S(=O)(O)=O)C=C5)=CC(S(=O)(O[Na])=O)=C1N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (135.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3555 mL 6.7775 mL 13.5551 mL
5 mM 0.2711 mL 1.3555 mL 2.7110 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3555 mL 6.7775 mL 13.5551 mL 33.8877 mL
5 mM 0.2711 mL 1.3555 mL 2.7110 mL 6.7775 mL
10 mM 0.1356 mL 0.6778 mL 1.3555 mL 3.3888 mL
15 mM 0.0904 mL 0.4518 mL 0.9037 mL 2.2592 mL
20 mM 0.0678 mL 0.3389 mL 0.6778 mL 1.6944 mL
25 mM 0.0542 mL 0.2711 mL 0.5422 mL 1.3555 mL
30 mM 0.0452 mL 0.2259 mL 0.4518 mL 1.1296 mL
40 mM 0.0339 mL 0.1694 mL 0.3389 mL 0.8472 mL
50 mM 0.0271 mL 0.1356 mL 0.2711 mL 0.6778 mL
60 mM 0.0226 mL 0.1130 mL 0.2259 mL 0.5648 mL
80 mM 0.0169 mL 0.0847 mL 0.1694 mL 0.4236 mL
100 mM 0.0136 mL 0.0678 mL 0.1356 mL 0.3389 mL
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Aniline Blue sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Aniline Blue sodium
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HY-W133919
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