AMPPD
Based on 1 publication(s) in Google Scholar
AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields.
For research use only. We do not sell to patients.
- Purity: 98.29%
- CAS No.: 122341-56-4
- Formula: C18H23O7P
- Molecular Weight:382.34
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
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Human Metabolite Library
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MCE Bioactive Compound Library
HY-L001V
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High-Throughput Bioactive Compound Library
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Mass Spectrometry Human Metabolite Library
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Publications Citing Use of MedChemExpress (MCE) AMPPD
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Biological Activity
AMPPD (0.4 mM; 20 min) can be used as a substrate in a chemiluminescent enzyme-linked immunosorbent assay to detect human tissue kininogen. The results showed that the detection sensitivity can reach 1 pM, the calibration curve concentration range is 0.8 pM to 456 pM, and there is no interference from the biological matrix[1].
Compared with other chemiluminescent substrates (CSPD, LUMI-PHOS 530), AMPPD (0.4 mM; 20 min) produces comparable luminescent signal intensities for immunoreactive substances at the same concentration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 122341-56-4
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Appearance Solid
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Molecular Weight 382.34
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Formula C18H23O7P
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Color White to off-white
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SMILES
COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1
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Synonyms
Lumi-Phos Plus; Lumigen PPD; PPD
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
High-affinity VNARs targeting human hemoglobin: Screening, stability and binding analysis. [Abstract]2025 Apr:299:140290. PMID: 39863199
Solvent & Solubility
H2O : 175 mg/mL (457.71 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Legris F, et al. An ultrasensitive chemiluminoenzyme immunoassay for the quantification of human tissue kininogens: application to synovial membrane and cartilage. J Immunol Methods. 1994 Jan 12;168(1):111-21. [Content Brief]
[2]. Green MR, et al. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes. Cold Spring Harb Protoc. 2022 Jan 4;2022(1). [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.6155 mL | 13.0774 mL | 26.1547 mL | 65.3868 mL |
| 5 mM | 0.5231 mL | 2.6155 mL | 5.2309 mL | 13.0774 mL | |
| 10 mM | 0.2615 mL | 1.3077 mL | 2.6155 mL | 6.5387 mL | |
| 15 mM | 0.1744 mL | 0.8718 mL | 1.7436 mL | 4.3591 mL | |
| 20 mM | 0.1308 mL | 0.6539 mL | 1.3077 mL | 3.2693 mL | |
| 25 mM | 0.1046 mL | 0.5231 mL | 1.0462 mL | 2.6155 mL | |
| 30 mM | 0.0872 mL | 0.4359 mL | 0.8718 mL | 2.1796 mL | |
| 40 mM | 0.0654 mL | 0.3269 mL | 0.6539 mL | 1.6347 mL | |
| 50 mM | 0.0523 mL | 0.2615 mL | 0.5231 mL | 1.3077 mL | |
| 60 mM | 0.0436 mL | 0.2180 mL | 0.4359 mL | 1.0898 mL | |
| 80 mM | 0.0327 mL | 0.1635 mL | 0.3269 mL | 0.8173 mL | |
| 100 mM | 0.0262 mL | 0.1308 mL | 0.2615 mL | 0.6539 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AMPPD Related Classifications
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