2225748-05-8
Chemical Structure
SYBR green I chloride
- CAS No.: 2225748-05-8
- Formula:C32H37ClN4S
- Molecular Weight:545.18
IUPAC Name: (E)-2-((3-(dimethylamino)propyl)(propyl)amino)-4-((3-methylbenzo[d]thiazol-2(3H)-ylidene)methyl)-1-phenylquinolin-1-ium chloride
InChIKey: IHHRNZWXTFDDLM-UHFFFAOYSA-M
SMILES: CCCN(CCCN(C)C)C(C=C1/C=C2N(C)C3=C(C=CC=C3)S\2)=[N+](C4=C1C=CC=C4)C5=CC=CC=C5.[Cl-]
Biological Activity:
SYBR Green I chloride is a highly sensitive fluorescent nucleic acid dye that binds specifically to the minor groove of double-stranded DNA or intercalates between base pairs. SYBR Green I chloride exhibits weak fluorescence in the unbound state but emits bright fluorescence upon binding, and it preferentially binds to large-fragment DNA and DNA with high G+C content. SYBR Green I chloride is suitable for real-time PCR technology; its fluorescence intensity correlates with the amount and size of amplification products, enabling accurate quantification of gene expression and discrimination of amplicons via melting curve analysis without additional post-processing. SYBR Green I chloride is widely used in preclinical in vitro nucleic acid detection[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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SYBR green I chloride | 98.09% | SYBR Green I chloride is a highly sensitive fluorescent nucleic acid dye that binds specifically to the minor groove of double-stranded DNA or intercalates between base pairs. SYBR Green I chloride exhibits weak fluorescence in the unbound state but emits bright fluorescence upon binding, and it preferentially binds to large-fragment DNA and DNA with high G+C content. SYBR Green I chloride is suitable for real-time PCR technology; its fluorescence intensity correlates with the amount and size of amplification products, enabling accurate quantification of gene expression and discrimination of amplicons via melting curve analysis without additional post-processing. SYBR Green I chloride is widely used in preclinical in vitro nucleic acid detection. |
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- [1]. Ramos-Payán R, et al. Quantification of cytokine gene expression using an economical real-time polymerase chain reaction method based on SYBR Green I. Scand J Immunol. 2003;57(5):439-445. [Content Brief]
- [2]. Giglio S, et al. Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR. Nucleic Acids Res. 2003;31(22):e136. [Content Brief]
Keywords
