Search Result

Results for "

pH sensitive

" in MedChemExpress (MCE) Product Catalog:

By Targets:	HIF/HIF Prolyl-Hydroxylase (1) Antibody-Drug Conjugates (ADCs) (1) Autophagy (1) Biochemical Assay Reagents (14) Drug Derivative (1) Drug-Linker Conjugates for ADC (1) Endonuclease (1) Environmental Pollutants (3) Fluorescent Dye (32) HIV (1) HIV Protease (1) Influenza Virus (1) Liposome (4) Microtubule/Tubulin (1) MMP (1) Pim (1) PSMA (1) Ser/Thr Protease (1)
By Research Areas:	Cancer (12) Infection (4) Inflammation/Immunology (3) Metabolic Disease (1) Neurological Disease (2)
Click Chemistry:	Labeling and Fluorescence Imaging (1) Alkynes (1)
Oligonucleotides:	Cationic Lipids (4)

Inhibitors & Agonists

Screening Libraries

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Natural
Products

Click Chemistry

Oligonucleotides

Targets Recommended: HIF/HIF Prolyl-Hydroxylase Hormone-Sensitive Lipase (HSL) VAP-1

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D1563

pH Receptor Probe 1 Publications Verification	Fluorescent Dye	Others
pH Receptor Probe is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. pH Receptor Probe has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. pH Receptor Probe has been used as a pH sensor of lysosomal compartments .

HY-P4108

TAT-HA2 Fusion Peptide	Influenza Virus	Infection
TAT-HA2 Fusion Peptide is a peptide-based delivery agent that combines the pH-sensitive HA2 fusion peptide from Influenza and the cell-penetrating peptide TAT from HIV. TAT-HA2 Fusion Peptide is a transactivator of transcription and hemaglutanin for endosomal release. TAT-HA2 Fusion Peptide enhances cellular uptake of macromolecules .

HY-D0023

Pyranine 2 Publications Verification HPTS; Solvent Green 7	Environmental Pollutants Fluorescent Dye	Others
Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D1094

Carboxy-SNARF 1 (5/6-mixture) 1 Publications Verification SNARF 1	Fluorescent Dye	Others
Carboxy-SNARF 1 (5/6-mixture) (SNARF 1) is a fluorescent probe that is sensitive to pH (Ex: 488 nm). Carboxy-SNARF 1 (5/6-mixture) can be used for measurement pH. Carboxy-SNARF 1 (5/6-mixture) exhibits a significant emission shift from yellow-orange (Em: 580 nm) to deep red fluorescence (Em: 640 nm) under acidic and basic conditions (pH=7-8), respectively .

HY-D1224

Chlorophenol red-β-D-galactopyranoside 2 Publications Verification CPRG	Biochemical Assay Reagents	Others
Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .

HY-101882

BCECF 3 Publications Verification 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein	Fluorescent Dye	Others
BCECF is a cell-impermeant *pH-sensitive* fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

HY-P99681

Labetuzumab govitecan IMMU 130; hMN-14-SN-38	Antibody-Drug Conjugates (ADCs)	Cancer
Labetuzumab govitecan (IMMU 130) is an Anti-CEACAM5/SN-38 antibody-drug conjugate (ADC). Labetuzumab govitecan consists of the antibody Labetuzumab, SN-38, and a pH-sensitive Linker. Labetuzumab govitecan can be used for research of cancers, such as colorectal cancer .

HY-15940

5(6)-Carboxyfluorescein 5 Publications Verification 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers	Fluorescent Dye	Cancer
5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo .

HY-P10152

INF7	Biochemical Assay Reagents	Infection
INF7 is a derivative of the N-terminal domain of the HA2 protein and is sensitive to pH. INF7 disrupts the stability of endosomal membranes through a mechanism independent of membrane fusion. INF7 can be used to enhance the endosome escape of complex or liposome-encapsulated proteins. Co-encapsulation of INF7 and molecular imaging probes in liposomes can enhance intracellular signaling and probe retention .

HY-W040144

Bromocresol green	Fluorescent Dye	Others
Bromocresol green is a pH-sensitive triphenylmethane dye commonly used for the determination of protein and albumin in serum. Bromocresol green is a bio-based dye with a yellow-green to blue-green color. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in acidic solution (e.g. pH=4.15), and turns blue in basic solution (λmax=615 nm, deprotonated form). Bromocresol green is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells .

HY-118155

9-Amino-6-chloro-2-methoxyacridine 2 Publications Verification ACMA	Fluorescent Dye	Others
9-Amino-6-chloro-2-methoxyacridine is a pH sensitive fluorescent probe. 9-Amino-6-chloro-2-methoxyacridine has been frequently used to measure changes in vacuolar pH when a specific substrate crosses the tonoplast through a putative H ⁺/solute antiport system .

HY-Y0699

Methyl red	Environmental Pollutants Fluorescent Dye	Others
Methyl red is a pH-sensitive acid-base indicator and colorimetric reagent with a pKa of 5.1. Methyl red achieves visual judgment of the titration endpoint through reversible structural changes of protonation (red, pH <4.4) and deprotonation (yellow, pH >6.2). Methyl red is widely used in acid-base titration in chemical analysis and buffer pH monitoring in biochemistry .

HY-D0077

2',7'-Difluorofluorescein Oregon green 488	Fluorescent Dye	Others
2',7'-Difluorofluorescein (Oregon green 488) is a fluorescein derivative and a pH-sensitive fluorescent probe (pK_a ~4.7). Upon excitation at 488 nm, 2',7'-Difluorofluorescein exhibits pH-sensitive fluorescence intensity through the formation of dianions, while its pH sensitivity decreases under excitation at 450 nm, allowing its use in ratiometric pH analysis. 2',7'-Difluorofluorescein can be used for the quantitative analysis of pH values in the range of 2-7 in submicron aerosol particles. 2',7'-Difluorofluorescein undergoes buffer-mediated and buffer-free excited-state proton transfer between different protonated forms, and its cationic form undergoes rapid excited-state deprotonation. 2',7'-Difluorofluorescein is resistant to photodegradation, maintains stable absorption and fluorescence properties within the physiological pH range, and serves as a fluorescent protein label, a component of Ca ²⁺ indicators, a fluorescent imaging agent, and an anisotropy probe .

HY-139305

CL4H6 1 Publications Verification	Liposome	Cancer
CL4H6 is a pH-sensitive cationic lipid. CL4H6 is the main component of lipid nanoparticles (LNPs), which can be used to target and deliver siRNA, and induces a potent gene-silencing response .

HY-W923198

Fluorescein O-methacrylate Methacryloyloxy fluorescein	Fluorescent Dye	Others
Fluorescein O-methacrylate (Methacryloyloxy fluorescein) is a pH-responsive fluorescent monomeric dye. Fluorescein O-methacrylate exhibits pH-responsive fluorescence properties: its fluorescence is activated under alkaline conditions and quenched under neutral or acidic conditions; reversible switching of fluorescence is achievable by adjusting the pH of the solution. Fluorescein O-methacrylate can be used as a monomer for the synthesis of amphiphilic fluorescein polymers. These polymers can self-assemble into stable polymer nanoparticles with enhanced aqueous-phase fluorescence, and also enable the conjugation of nanoparticles with amine-containing biomolecules to support sensitive optical detection of biomarkers (Ex/Em = 490/520 nm) .

HY-W133967

Calcium alginate	Environmental Pollutants Biochemical Assay Reagents	Inflammation/Immunology
Calcium alginate is a biopolymer that can be used for developing oral agent-delivery systems . Calcium alginate is a pH sensitive hydrogel stable in acidic media and soluble in basic media .

HY-D0075

DHPDS disodium salt	Fluorescent Dye	Others
DHPDS disodium salt is a pH-sensitive fluorescent dye. DHPDS disodium salt is used to measure intracellular pH (pHi) from the surface fluorescence of the isolated perfused rat liver .

HY-124473

Naphthofluorescein NapHthafluorescein	HIF/HIF Prolyl-Hydroxylase	Cancer
Naphthofluorescein inhibits the interaction between HIF-1 and Mint3. Naphthofluorescein suppresses Mint3-dependent HIF-1 activity and glycolysis in cancer cells and macrophages without cytotoxicity in vitro and adverse effect in vivo . Naphthofluorescein is also a fluorescent pH-sensitive probe that can be used for functional Cerenkov imaging .

HY-147087

YSK 05	Liposome	Cancer
YSK 05 is a pH-sensitive cationic lipid. YSK 05 improves the intracellular trafficking of non-viral vectors. YSK 05-MEND shows significantly good gene silencing activity and hemolytic activity. YSK 05 overcomes the suppression of endosomal escape by PEGylation. YSK 05 effectively enhances siRNA delivery both in vitro and in vivo .

HY-W110898

Nile blue chloride	Fluorescent Dye	Others
Nile blue chloride is a highly fluorescent and photostable organic dye. Nile blue chloride and fluorescein isothiocyanate (FITC) can be used to construct a ratiometric pH sensitive probe for tracking the pH of the extracellular fluid between cancer cells in realtime. Nile Blue chloride has the potential for the research of nonlinear optics .

HY-D1457

DND-189	Fluorescent Dye	Others
DND-189, a low-pH fluorescent probe, is sensitive to neutral and low pH range. DND-189 can be used to measure the pH of acidic organelles .

HY-D1093

Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture)	Fluorescent Dye	Others
Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) is a fluorescent probe that is sensitive to pH. Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) can be used for measurement of intracellular pH .

HY-W094214

Monomethyl itaconate β-Methyl itaconate	Drug Derivative	Others
Monomethyl itaconate (β-Methyl itaconate) is a monomethyl ester derivative of Itaconic acid (HY-Y0520). Monomethyl itaconate can copolymerize with PEGMA to form pH-sensitive hydrogels .

HY-DY1071

pH Receptor Probe (solution)	Fluorescent Dye	Others
pH Receptor Probe (solution) is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. pH Receptor Probe has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. pH Receptor Probe has been used as a pH sensor of lysosomal compartments . Solvent and concentration: DMSO: 10 mM

HY-157036

INSA	Fluorescent Dye	Others
INSA is a a pH-sensitive fluorescent probe. INSA can simultaneously light up mitochondria (red emission) and lysosomes (green emission) for their internal pH differences .

HY-144025

DOBAQ	Liposome	Others
DOBAQ, a cationic lipid, is a pH-sensitive lipid. DOBAQ can be used for liposomes applied in drug delivery .

HY-160113E

Sodium phosphate buffer 0.1M, pH 8.0	Biochemical Assay Reagents	Others
Sodium phosphate buffer 0.1M, pH 8.0 is an aqueous sodium phosphate buffer with a concentration of 0.1M and a pH value of 8.0. Sodium phosphate buffer 0.1M, pH 8.0 can be used for the preparation of hydrated micellar membranes, the construction of pH-sensitive nanocarriers, and the maintenance of stable environmental conditions for pH-cleavable PEG-Hz-PE micelles. Sodium phosphate buffer 0.1M, pH 8.0 can also serve as a universal solvent, reaction medium and eluent, and is widely applied in experimental processes such as antibody thiolation, nanoparticle purification and cross-linking reactions .

HY-D1556

18:1 PE CF 1,2-dioleoyl-sn-glycero-3-pHospHoethanolamine-N-(carboxyfluorescein) ammonium	Fluorescent Dye	Cancer
18:1 PE CF (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium)) is a pH-sensitive fluorescent indicator. 18:1 PE CF exhibits changes in fluorescence intensity in response to pH variations and reflects the electrostatic states of cationic liposomes and lipoplexes. 18:1 PE CF supports real-time visualization of pH distribution and membrane deformation in giant unilamellar vesicles and is suitable for steady-state fluorescence testing in large unilamellar vesicle membranes. 18:1 PE CF is capable of monitoring the surface electrical potential of cationic liposomes and lipoplexes and can effectively label lipid bilayers and nanomicelles. 18:1 PE CF can be used for the research of the investigation of lung cancer (Ex = 495 nm; Em = 520 nm) .

HY-D2482

Asante potassium green-1 AM APG-1 AM	Fluorescent Dye	Neurological Disease
Asante potassium green-1 AM (APG-1 AM) is a cell-permeable K ⁺-sensitive fluorescent indicator and potassium ion indicator that exhibits minimal interference from Na ⁺ and pH at physiologically relevant intracellular levels. Asante potassium green-1 AM enables non-invasive monitoring and quantitative analysis of intracellular potassium ion concentration (ᵢ) . Its excitation/emission maxima are 525/545 nm, respectively.

HY-20794

8-Hydroxyjulolidine	Fluorescent Dye	Others
8-Hydroxyjulolidine is a chromophore moiety of a fluorescent probe for the detection of weak acid ions. 8-Hydroxyjulolidine can be used to synthesize the salicylaldehyde derivative 9-formyl-8-hydroxyjulolidine, which exhibits sensitive detection ability to pH changes of hydrogen sulfide and silicate in aqueous solution .

HY-E70565

O-Glycoprotease	Endonuclease	Inflammation/Immunology
O-Glycoprotease is an O-glycoprotein-specific endoprotease that catalyzes the hydrolysis of peptide bonds directly adjacent to the O-polymer in native mucin-type O-glycosylated proteins. O-Glycoprotease sequence is from Akkermansia muciniphila, recombinantly expressed in E.coli, with a 6×His tag at the C-terminus. The enzyme maintains high activity between pH 5.5-7.5 and is resistant to 1M NaCl, but is highly sensitive to EDTA (0.5 mM EDTA) and can be inhibited by Zn ²⁺.

HY-150175

HKSOX-1	Fluorescent Dye	Inflammation/Immunology
HKSOX-1 and its derivatives (HKSOX-1r and HKSOX-1m) are novel fluorescent probes designed for highly sensitive and selective detection of the superoxide anion radical (O₂ ^•−) in cellular environments. These probes utilize an aryl trifluoromethanesulfonate group that undergoes O₂ ^•−-mediated cleavage, releasing a free phenol and emitting fluorescence. They demonstrate excellent specificity and sensitivity across various pH ranges, withstand interference from strong oxidants and reductants typical in cellular contexts. HKSOX-1r, optimized for cellular retention, has been effectively employed in diverse assays including confocal imaging, flow cytometry, and zebrafish embryo studies, highlighting its utility in investigating O₂ ^•− roles in inflammation, mitochondrial stress, and other physiological processes .

HY-D1463

Nile Blue Methacrylamide	Fluorescent Dye	Others
Nile Blue Methacrylamide is a pH-responsive probe. Nile Blue Methacrylamide exhibits pH-sensitive absorption and fluorescence emission in the physiological pH range, with spectral shifts occurring between acidic and near-physiological pH conditions . (Ex/Em = 590/670-700 nm)

HY-147104

Fmoc-Lys(5-FITC)-OH	Fluorescent Dye	Others
Fmoc-Lys(5-FITC)-OH is a marker for polypeptides or proteins. FITC is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu ²⁺-sensitive fluorescence dye .

HY-D2771

BP Fluor 405 NHS ester	Fluorescent Dye	Others
BP Fluor 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BP Fluor 405 conjugates are pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. BP Fluor 405 conjugates can also be used for the detection of abundance targets.

HY-156514

MC-GGFG-AM-(10NH2-11F-Camptothecin)	Drug-Linker Conjugates for ADC	Cancer
MC-GGFG-AM-(10NH2-11F-Camptothecin) is an antibody drug conjugates (ADC). MC-GGFG-AM-(10NH2-11F-Camptothecin) binds to the anti-TROp-2 antibody sacituzumab via a hydrolysable pH-sensitive linker and has anti-tumor activity. MC-GGFG-AM-(10NH2-11F-Camptothecin) can be used for cancer research .

HY-176518

ssPalmE-P4-C2	Liposome Ser/Thr Protease	Infection Neurological Disease Metabolic Disease
ssPalmE-P4-C2 is a SS-cleavable and pH-sensitive lipid-like material (ssPalm) with a vitamin E‑scaffold. ssPalmE-P4-C2 improves gene knockdown activity against FVII with an ED₅₀ of 0.5 mg/kg. ssPalmE-P4-C2 can be used to synthesize lipid nanoparticles (LNPs) for delivering siRNA to the hepatocyte. ssPalmE-P4-C2 can be used for the RNA therapies for dyslipidemia, hepatitis B/C infections and transthyretin amyloidosis research .

HY-P2815

Creatinine deiminase	Biochemical Assay Reagents	Others
Creatinine deiminase is a hydrolytic enzyme. Creatinine deiminase plays a crucial role in the conversion of the metabolic waste creatinine into creatine. Creatinine deiminase is a creatine bioanalytical tool. Creatinine deiminase can be used to construct a novel creatinine-sensitive biosensor when adsorbed on a silicate-modified pH-sensitive field-effect transistor (pH-FET) .

HY-W040291

7-Hydroxy-4-methylcoumarin-3-acetic acid, SE	Fluorescent Dye	Cancer
7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is a blue fluorophore that has pH-dependent and environment-sensitive fluorescence. It is widely used for preparing bioconjugates of blue fluorescence.

HY-168913

CTT2274	PSMA	Cancer
CTT2274 is a prodrug of MMAE (HY-15162). CTT2274 is composed of a prostate-specific membrane antigen (PSMA)-binding scaffold, a biphenyl motif, a pH-sensitive phosphoramidate linker, and MMAE payload. CTT2274 shows selective binding to PSMA and delivers MMAE. CTT2274 inhibits prostate cancer .

HY-D2745

BP Fluor 594 NHS ester	Fluorescent Dye	Others
BP Fluor 594 NHS ester is the most popular tool for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with BP Fluor 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. BP Fluor 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. BP Fluor 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. BP Fluor 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

HY-D1224A

Chlorophenol red β-D-galactopyranoside sodium CPRG sodium	Biochemical Assay Reagents	Others
Chlorophenol red-β-D-galactopyranoside (CPRG) sodium is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside sodium itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .

HY-126470A

N-Palmitoyl homocysteine (PHC) ammonium pHCPamHcy	Biochemical Assay Reagents	Others
N-Palmitoyl homocysteine (PHC) ammonium is a pH sensitive lipid hat is cleaved under endosomal pH conditions (pH 5.5) .

HY-145616

Sudocetaxel	Microtubule/Tubulin	Cancer
Sudocetaxel is a microtubule depolymerization inhibitor for pH-sensitive docetaxel delivery.

HY-W353607A

Bromochlorophenol Blue sodium salt	Biochemical Assay Reagents	Others
Bromochlorophenol Blue sodium salt, an acid-sensitive dye, is a pH-indicating dye .

HY-172385

16:0 DGS 1,2-Dipalmitoyl-sn-glycero-3-succinate	Biochemical Assay Reagents	Others
16:0 DGS (1,2-Dipalmitoyl-sn-glycero-3-succinate) can be used for the preparation of pH-sensitive liposomes .

HY-129563

CGP 57813	HIV HIV Protease	Infection
CGP 57813 is a peptidomimetic inhibitor of HIV protease that can be encapsulated by nanoparticles composed of poly(D,L-lactic acid) (PLA) and pH-sensitive methacrylic acid copolymers and delivered into the body .

HY-D0023A

Pyranine hydrate HPTS hydrate; Solvent Green 7 hydrate	Fluorescent Dye	Others
Pyranine (HPTS; Solvent Green 7) hydrate is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine hydrate acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine hydrate is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D0116

Pyranine free acid HPTS free acid; Solvent Green 7 free acid	Fluorescent Dye	Others
Pyranine (HPTS; Solvent Green 7) free acid is a pH-sensitive fluorescent indicator. Pyranine free acid acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine free acid is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-155717

NDBM	Autophagy Pim	Cancer
NDBM is a probe and binds to tumor-specific Pim-1 kinase, releases strong fluorescence, and produces cytotoxicity, thus achieving cell screening and killing effects. NDBM can specifically target lysosomes and sensitively respond to pH. NDBM can be used to track the pH changes in the intracellular environment under conditions of autophagy and external stimulation .

Cat. No.	Product Name

HY-L251

Ionizable Lipid Compound Library

95 compounds

Ionizable lipids are a class of specialized, functional lipid molecules with pH-sensitive charge characteristics. They are primarily divided into two major categories: ionizable cationic lipids and ionizable anionic lipids, though the term typically specifies ionizable cationic lipids within the biomedical field. Structurally, these lipids consist of an ionizable hydrophilic headgroup, a biodegradable linker, and hydrophobic tails. Their primary application is serving as the key delivery vehicle in lipid nanoparticles (LNPs) to encapsulate negatively charged nucleic acid macromolecules, such as mRNA vaccines, siRNA therapeutics, and CRISPR gene-editing components. In a physiological, neutral environment, they remain electrically neutral to minimize systemic toxicity and prolong circulation time. Upon entering the acidic microenvironment of cellular endosomes, however, they undergo protonation to become positively charged, thereby inducing membrane fusion and enabling the highly efficient intracellular release of the nucleic acid cargo. Consequently, they serve as the technological cornerstone for bringing nucleic acid therapies into clinical application.

To accelerate the translational process of cutting-edge nucleic acid drugs, MCE has meticulously constructed an ionizable lipid compound library containing 95 high-performance molecules, aiming to provide researchers and pharmaceutical professionals with a high-throughput, multi-dimensional lipid screening platform.

Cat. No.	Product Name	Type

HY-D1563

pH Receptor Probe 1 Publications Verification	Fluorescent Dye
pH Receptor Probe is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. pH Receptor Probe has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. pH Receptor Probe has been used as a pH sensor of lysosomal compartments .

HY-D0023

Pyranine 2 Publications Verification HPTS; Solvent Green 7	Fluorescent Dye
Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D1094

Carboxy-SNARF 1 (5/6-mixture) 1 Publications Verification SNARF 1	Fluorescent Dye
Carboxy-SNARF 1 (5/6-mixture) (SNARF 1) is a fluorescent probe that is sensitive to pH (Ex: 488 nm). Carboxy-SNARF 1 (5/6-mixture) can be used for measurement pH. Carboxy-SNARF 1 (5/6-mixture) exhibits a significant emission shift from yellow-orange (Em: 580 nm) to deep red fluorescence (Em: 640 nm) under acidic and basic conditions (pH=7-8), respectively .

HY-D1224

Chlorophenol red-β-D-galactopyranoside

2 Publications Verification

CPRG

Fluorescent Dye

Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .

HY-101882

BCECF 3 Publications Verification 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein	Fluorescent Dye
BCECF is a cell-impermeant *pH-sensitive* fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

HY-15940

5(6)-Carboxyfluorescein 5 Publications Verification 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers	Fluorescent Dye
5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo .

HY-118155

9-Amino-6-chloro-2-methoxyacridine 2 Publications Verification ACMA	Fluorescent Dye
9-Amino-6-chloro-2-methoxyacridine is a pH sensitive fluorescent probe. 9-Amino-6-chloro-2-methoxyacridine has been frequently used to measure changes in vacuolar pH when a specific substrate crosses the tonoplast through a putative H ⁺/solute antiport system .

HY-W923198

Fluorescein O-methacrylate

Methacryloyloxy fluorescein

Fluorescent Dye

Fluorescein O-methacrylate (Methacryloyloxy fluorescein) is a pH-responsive fluorescent monomeric dye. Fluorescein O-methacrylate exhibits pH-responsive fluorescence properties: its fluorescence is activated under alkaline conditions and quenched under neutral or acidic conditions; reversible switching of fluorescence is achievable by adjusting the pH of the solution. Fluorescein O-methacrylate can be used as a monomer for the synthesis of amphiphilic fluorescein polymers. These polymers can self-assemble into stable polymer nanoparticles with enhanced aqueous-phase fluorescence, and also enable the conjugation of nanoparticles with amine-containing biomolecules to support sensitive optical detection of biomarkers (Ex/Em = 490/520 nm) .

HY-D0075

DHPDS disodium salt	Fluorescent Dye
DHPDS disodium salt is a pH-sensitive fluorescent dye. DHPDS disodium salt is used to measure intracellular pH (pHi) from the surface fluorescence of the isolated perfused rat liver .

HY-D1457

DND-189	Fluorescent Dye
DND-189, a low-pH fluorescent probe, is sensitive to neutral and low pH range. DND-189 can be used to measure the pH of acidic organelles .

HY-D1093

Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture)	Fluorescent Dye
Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) is a fluorescent probe that is sensitive to pH. Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) can be used for measurement of intracellular pH .

HY-DY1071

pH Receptor Probe (solution)	Fluorescent Dye
pH Receptor Probe (solution) is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. pH Receptor Probe has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. pH Receptor Probe has been used as a pH sensor of lysosomal compartments . Solvent and concentration: DMSO: 10 mM

HY-157036

INSA	Fluorescent Dye
INSA is a a pH-sensitive fluorescent probe. INSA can simultaneously light up mitochondria (red emission) and lysosomes (green emission) for their internal pH differences .

HY-D1556

18:1 PE CF

1,2-dioleoyl-sn-glycero-3-pHospHoethanolamine-N-(carboxyfluorescein) ammonium

Fluorescent Dye

18:1 PE CF (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium)) is a pH-sensitive fluorescent indicator. 18:1 PE CF exhibits changes in fluorescence intensity in response to pH variations and reflects the electrostatic states of cationic liposomes and lipoplexes. 18:1 PE CF supports real-time visualization of pH distribution and membrane deformation in giant unilamellar vesicles and is suitable for steady-state fluorescence testing in large unilamellar vesicle membranes. 18:1 PE CF is capable of monitoring the surface electrical potential of cationic liposomes and lipoplexes and can effectively label lipid bilayers and nanomicelles. 18:1 PE CF can be used for the research of the investigation of lung cancer (Ex = 495 nm; Em = 520 nm) .

HY-D2482

Asante potassium green-1 AM

APG-1 AM

Fluorescent Dye

Asante potassium green-1 AM (APG-1 AM) is a cell-permeable K ⁺-sensitive fluorescent indicator and potassium ion indicator that exhibits minimal interference from Na ⁺ and pH at physiologically relevant intracellular levels. Asante potassium green-1 AM enables non-invasive monitoring and quantitative analysis of intracellular potassium ion concentration (ᵢ) . Its excitation/emission maxima are 525/545 nm, respectively.

HY-D1463

Nile Blue Methacrylamide	Fluorescent Dye
Nile Blue Methacrylamide is a pH-responsive probe. Nile Blue Methacrylamide exhibits pH-sensitive absorption and fluorescence emission in the physiological pH range, with spectral shifts occurring between acidic and near-physiological pH conditions . (Ex/Em = 590/670-700 nm)

HY-D2771

BP Fluor 405 NHS ester

Fluorescent Dye

BP Fluor 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BP Fluor 405 conjugates are pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. BP Fluor 405 conjugates can also be used for the detection of abundance targets.

HY-W040291

7-Hydroxy-4-methylcoumarin-3-acetic acid, SE	Fluorescent Dye
7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is a blue fluorophore that has pH-dependent and environment-sensitive fluorescence. It is widely used for preparing bioconjugates of blue fluorescence.

HY-D2745

BP Fluor 594 NHS ester

Fluorescent Dye

BP Fluor 594 NHS ester is the most popular tool for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with BP Fluor 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. BP Fluor 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. BP Fluor 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. BP Fluor 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

HY-W353607A

Bromochlorophenol Blue sodium salt	Fluorescent Dye
Bromochlorophenol Blue sodium salt, an acid-sensitive dye, is a pH-indicating dye .

HY-D0023A

Pyranine hydrate HPTS hydrate; Solvent Green 7 hydrate	Fluorescent Dye
Pyranine (HPTS; Solvent Green 7) hydrate is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine hydrate acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine hydrate is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D0116

Pyranine free acid HPTS free acid; Solvent Green 7 free acid	Fluorescent Dye
Pyranine (HPTS; Solvent Green 7) free acid is a pH-sensitive fluorescent indicator. Pyranine free acid acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine free acid is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D1593

BODIPY TMR-X alkyne	Fluorescent Dye
BODIPY TMR-X alkyne, an orange-fluorescent dye, is an alkyne modified BODIPY TMR-X. BODIPY TMR-X alkyne can react with azides by the copper-catalyzed click reaction. BODIPY TMR-X alkyne is not sensitive to pH.

HY-D2768

BP Fluor 430 NHS ester

Fluorescent Dye

BP Fluor 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BP Fluor 405 conjugates are pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

HY-DY1098

BCECF (solution) 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (solution)	Fluorescent Dye
BCECF (solution) (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (solution)) is a cell-impermeant *pH-sensitive* fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm. Solvent and concentration: DMSO: 10 mM

HY-D2998

ONOO-LysopH

Fluorescent Dye

ONOO-LysopH is a new Rhodamine-based near-infrared fluorescent probe. ONOO-LysopH is acid-sensitive, exhibiting rapid near-infrared fluorescence enhancement around 686 nm under acidic pH conditions. ONOO-LysopH also displays a rapid NIR turn-on fluorescence around 678 nm for ONOO− at physiological pH. ONOO-LysopH can target lysosomes to track lysosomal pH changes and image endogenous/exogenous ONOO− in live cells. ONOO-LysopH is applicable for cell imaging ONOO− and tracking lysosomal pH changes .

Cat. No.	Product Name	Type

HY-W040144

Bromocresol green

Biochemical Assay Reagents

Bromocresol green is a pH-sensitive triphenylmethane dye commonly used for the determination of protein and albumin in serum. Bromocresol green is a bio-based dye with a yellow-green to blue-green color. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in acidic solution (e.g. pH=4.15), and turns blue in basic solution (λmax=615 nm, deprotonated form). Bromocresol green is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells .

HY-Y0699

Methyl red	Biochemical Assay Reagents
Methyl red is a pH-sensitive acid-base indicator and colorimetric reagent with a pKa of 5.1. Methyl red achieves visual judgment of the titration endpoint through reversible structural changes of protonation (red, pH <4.4) and deprotonation (yellow, pH >6.2). Methyl red is widely used in acid-base titration in chemical analysis and buffer pH monitoring in biochemistry .

HY-147087

YSK 05	Biochemical Assay Reagents
YSK 05 is a pH-sensitive cationic lipid. YSK 05 improves the intracellular trafficking of non-viral vectors. YSK 05-MEND shows significantly good gene silencing activity and hemolytic activity. YSK 05 overcomes the suppression of endosomal escape by PEGylation. YSK 05 effectively enhances siRNA delivery both in vitro and in vivo .

HY-144025

DOBAQ	Biochemical Assay Reagents
DOBAQ, a cationic lipid, is a pH-sensitive lipid. DOBAQ can be used for liposomes applied in drug delivery .

HY-160113E

Sodium phosphate buffer 0.1M, pH 8.0

Biochemical Assay Reagents

Sodium phosphate buffer 0.1M, pH 8.0 is an aqueous sodium phosphate buffer with a concentration of 0.1M and a pH value of 8.0. Sodium phosphate buffer 0.1M, pH 8.0 can be used for the preparation of hydrated micellar membranes, the construction of pH-sensitive nanocarriers, and the maintenance of stable environmental conditions for pH-cleavable PEG-Hz-PE micelles. Sodium phosphate buffer 0.1M, pH 8.0 can also serve as a universal solvent, reaction medium and eluent, and is widely applied in experimental processes such as antibody thiolation, nanoparticle purification and cross-linking reactions .

HY-D1224A

Chlorophenol red β-D-galactopyranoside sodium

CPRG sodium

Biochemical Assay Reagents

Chlorophenol red-β-D-galactopyranoside (CPRG) sodium is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside sodium itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .

HY-W353607A

Bromochlorophenol Blue sodium salt	Biochemical Assay Reagents
Bromochlorophenol Blue sodium salt, an acid-sensitive dye, is a pH-indicating dye .

HY-172385

16:0 DGS 1,2-Dipalmitoyl-sn-glycero-3-succinate	Biochemical Assay Reagents
16:0 DGS (1,2-Dipalmitoyl-sn-glycero-3-succinate) can be used for the preparation of pH-sensitive liposomes .

HY-203300D

DOPE-PEG10000-NHS

Biochemical Assay Reagents

DOPE-PEG1000-NHS is a conjugate composed of DOPE, PEG chains, and terminal active ester (NHS). The NHS ester in DOPE-PEG1000-NHS can rapidly covalently react with molecules containing primary amines (such as proteins, peptide chains, and small molecules modified with amino groups) to form stable amide bonds, suitable for the construction of carriers requiring membrane fusion properties or pH-sensitive release (such as gene delivery systems).

HY-203300C

DOPE-PEG5000-NHS

Biochemical Assay Reagents

DOPE-PEG5000-NHS is a conjugate composed of DOPE, PEG chains, and terminal active ester (NHS). The NHS ester in DOPE-PEG5000-NHS can rapidly covalently react with molecules containing primary amines (such as proteins, peptide chains, and small molecules modified with amino groups) to form stable amide bonds, suitable for the construction of carriers requiring membrane fusion properties or pH-sensitive release (such as gene delivery systems).

HY-203300B

DOPE-PEG3400-NHS

Biochemical Assay Reagents

DOPE-PEG3400-NHS is a conjugate composed of DOPE, PEG chains, and terminal active ester (NHS). The NHS ester in DOPE-PEG3400-NHS can rapidly covalently react with molecules containing primary amines (such as proteins, peptide chains, and small molecules modified with amino groups) to form stable amide bonds, suitable for the construction of carriers requiring membrane fusion properties or pH-sensitive release (such as gene delivery systems).

HY-203300A

DOPE-PEG1000-NHS

Biochemical Assay Reagents

HY-203300

DOPE-PEG2000-NHS

Biochemical Assay Reagents

DOPE-PEG2000-NHS is a conjugate composed of DOPE, PEG chains, and terminal active ester (NHS). The NHS ester in DOPE-PEG2000-NHS can rapidly covalently react with molecules containing primary amines (such as proteins, peptide chains, and small molecules modified with amino groups) to form stable amide bonds, suitable for the construction of carriers requiring membrane fusion properties or pH-sensitive release (such as gene delivery systems).

Cat. No.	Product Name	Target	Research Area

HY-P4108

TAT-HA2 Fusion Peptide	Influenza Virus	Infection
TAT-HA2 Fusion Peptide is a peptide-based delivery agent that combines the pH-sensitive HA2 fusion peptide from Influenza and the cell-penetrating peptide TAT from HIV. TAT-HA2 Fusion Peptide is a transactivator of transcription and hemaglutanin for endosomal release. TAT-HA2 Fusion Peptide enhances cellular uptake of macromolecules .

HY-P10152

INF7	Biochemical Assay Reagents	Infection
INF7 is a derivative of the N-terminal domain of the HA2 protein and is sensitive to pH. INF7 disrupts the stability of endosomal membranes through a mechanism independent of membrane fusion. INF7 can be used to enhance the endosome escape of complex or liposome-encapsulated proteins. Co-encapsulation of INF7 and molecular imaging probes in liposomes can enhance intracellular signaling and probe retention .

HY-P5423A

GALA TFA	Peptides	Others
GALA (TFA) is a biological active peptide (GALA, a pH-sensitive fusogenic peptide, is a promising method to accelerate this step in order to enhance the expression of the desired proteins.) .

HY-P5405

dTAT-HA2 Transducible TAT-HA fusogenic peptide	Peptides	Others
dTAT-HA2 enhances TAT-Cre escape from macropinosomes. dTAT-HA2 is a transfusible, *pH-sensitive, fusogenic* peptide .

Cat. No.	Product Name		Classification

HY-D1593

BODIPY TMR-X alkyne		Labeling and Fluorescence Imaging Alkynes
BODIPY TMR-X alkyne, an orange-fluorescent dye, is an alkyne modified BODIPY TMR-X. BODIPY TMR-X alkyne can react with azides by the copper-catalyzed click reaction. BODIPY TMR-X alkyne is not sensitive to pH.

Cat. No.	Product Name		Classification

HY-139305

CL4H6 1 Publications Verification		Cationic Lipids
CL4H6 is a pH-sensitive cationic lipid. CL4H6 is the main component of lipid nanoparticles (LNPs), which can be used to target and deliver siRNA, and induces a potent gene-silencing response .

HY-147087

YSK 05		Cationic Lipids
YSK 05 is a pH-sensitive cationic lipid. YSK 05 improves the intracellular trafficking of non-viral vectors. YSK 05-MEND shows significantly good gene silencing activity and hemolytic activity. YSK 05 overcomes the suppression of endosomal escape by PEGylation. YSK 05 effectively enhances siRNA delivery both in vitro and in vivo .

HY-144025

DOBAQ		Cationic Lipids
DOBAQ, a cationic lipid, is a pH-sensitive lipid. DOBAQ can be used for liposomes applied in drug delivery .

HY-176518

ssPalmE-P4-C2		Cationic Lipids
ssPalmE-P4-C2 is a SS-cleavable and pH-sensitive lipid-like material (ssPalm) with a vitamin E‑scaffold. ssPalmE-P4-C2 improves gene knockdown activity against FVII with an ED₅₀ of 0.5 mg/kg. ssPalmE-P4-C2 can be used to synthesize lipid nanoparticles (LNPs) for delivering siRNA to the hepatocyte. ssPalmE-P4-C2 can be used for the RNA therapies for dyslipidemia, hepatitis B/C infections and transthyretin amyloidosis research .

Inquiry Online

Your information is safe with us. * Required Fields.

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Salutation			Country or Region *
Applicant Name *			Organization Name *
Department *
Email Address *			Product Name *
Cat. No.			Requested quantity *
Phone Number *
Remarks

Inquiry Online

Inquiry Information

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

pH sensitive

Inhibitors & Agonists

Screening Libraries

Fluorescent Dye

Biochemical Assay Reagents

Peptides

NaturalProducts

Click Chemistry

Oligonucleotides

Natural
Products