99792-79-7
Chemical Structure
Chlorophenol red-β-D-galactopyranoside
Synonym(s): CPRG
- CAS 番号: 99792-79-7
- Formula:C25H22Cl2O10S
- Molecular Weight:585.41
IUPAC Name: 3-(3-chloro-4-(((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-3-(3-chloro-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide
InChIKey: UYYRDZGZGNYVBA-VPXCCNNISA-N
SMILES: O=S1(OC(C2=CC(Cl)=C(C=C2)O[C@@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO)(C4=CC(Cl)=C(C=C4)O)C5=CC=CC=C51)=O
Biological Activity: Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry[1][2].
| 製品番号 | 製品名 | 純度 | 製品説明 | Pricing | |||||||||||||||||||
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Chlorophenol red-β-D-galactopyranoside | 99.5% | Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry. | ||||||||||||||||||||
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References
- [1]. Sicard C, et al. A rapid and sensitive fluorimetric β-galactosidase assay for coliform detection using chlorophenol red-β-D-galactopyranoside. Anal Bioanal Chem. 2014 Sep;406(22):5395-403. [Content Brief]
- [2]. Els Vanderperren, et al. Oestrogenic activity of CPRG (chlorophenol red-β-D-galactopyranoside), a β-galactosidase substrate commonly used in recombinant yeast oestrogenic assays. Biomarkers. 2001;6(5):375-80. [Content Brief]
