β-Galactosidase, E. coli
Based on 1 Customer Validation
β-Galactosidase, E. coli (EC 3.2.1.23; GAL) is a glycoside hydrolase that hydrolyzes the β-glycosidic bonds formed between galactose and its organic moieties. β-Galactosidase, E. coli can hydrolyze lactose to form glucose and galactose, and enter glycolysis; it can also catalyze the transgalactosylation of lactose into allolactose; allolactose can be cracked into monosaccharides.
For research use only. We do not sell to patients.
- CAS No.: 9031-11-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Inhibitors: Co2+, Cu2+, Ni2+, Zn2+, Proclin
pH range: Effective pH range 3.0-8.0, Optimum pH range 3.5-5.0
Temperature range: Effective temperature range 55°C-65°C, Optimum temperature range 55°C-60°C
Instructions
Enzyme solutions are susceptible to oxidation and should be prepared fresh. Oxidation can be minimized by preparing solutions in 50 mM Tris HCl, pH 7.3, 5- 10 mM β-mercaptoethanol, and 10 mM MgCl2.
Note: Thiol reducing agents should only be used in the presence of magnesium chloride, as β-mercaptoethanol will have an inhibitory effect when magnesium chloride is not present.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.2.1.23
Enzyme Activity
≥50U/mg soild
Unit Definition
One unit defined as the amount of enzyme that causes the formation of one micromole of ONP per min at pH 7.3 at 37℃.
Chemical Information
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CAS No. 9031-11-2
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Appearance Solid
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Color White to light yellow
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SMILES
[b-Galactosidase, E. coli]
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Synonyms
EC 3.2.1.23; GAL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
β-Galactosidase, E. coli Related Classifications
HY-P2869 Related Classifications
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