β1-3 Galactosidase 

β1-3 Galactosidase is a GH42 exoglycosidase that specifically hydrolyzes terminal β1-3-linked galactose. β1-3 Galactosidase uses Glu164 as the acid/base catalyst and Glu324 as the catalytic nucleophile to hydrolyze β1-3 galactosidic linkages. β1-3 Galactosidase can be used for research on galactoside metabolism^[1][2].

β1-3 Galactosidase efficiently hydrolyzes unmodified β1-3 and β1-6 galactosidic linkages and shows low activity toward β1-4 galactosidic linkages and N-acetylated β1-3 galactosides^[1].
β1-3 Galactosidase exhibits optimal catalytic activity at pH 5.5 and 45°C in vitro^[1].
β1-3 Galactosidase (Glu324Ala mutant enzyme) shows significantly decreased catalytic activity compared with the wild-type enzyme^[1].
β1-3 Galactosidase (10 Units; 120 min), when combined with β1-4 Galactosidase S, removes terminal galactose residues from bovine fetuin's glycans in vitro^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9031-11-2

Liquid (Density: 1.14 g/cm³)

Colorless to light yellow

[b1-3 Galactosidase]

≥1000U/mL

One unit is defined as the amount of enzyme that releases 1 nmol of galactose per minute from Gal β1-3GlcNAcβ1-3Galβ1-4Glc at 37°C, pH 5.0

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Viborg AH, et al. A β1-6/β1-3 galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 gives insight into sub-specificities of β-galactoside catabolism within Bifidobacterium. Mol Microbiol. Published online October 7, 2014.  [Content Brief]

  [2]. Saunders MJ, et al. Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering. Sci Rep. 2023;13(1):567. Published 2023 Jan 11.