β1-3 Galactosidase
Based on 1 Customer Validation
β1-3 Galactosidase is a GH42 exoglycosidase that specifically hydrolyzes terminal β1-3-linked galactose. β1-3 Galactosidase uses Glu164 as the acid/base catalyst and Glu324 as the catalytic nucleophile to hydrolyze β1-3 galactosidic linkages. β1-3 Galactosidase can be used for research on galactoside metabolism.
For research use only. We do not sell to patients.
- CAS No.: 9031-11-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Glucosidase |
β1-3 Galactosidase efficiently hydrolyzes unmodified β1-3 and β1-6 galactosidic linkages and shows low activity toward β1-4 galactosidic linkages and N-acetylated β1-3 galactosides[1].
β1-3 Galactosidase exhibits optimal catalytic activity at pH 5.5 and 45°C in vitro[1].
β1-3 Galactosidase (Glu324Ala mutant enzyme) shows significantly decreased catalytic activity compared with the wild-type enzyme[1].
β1-3 Galactosidase (10 Units; 120 min), when combined with β1-4 Galactosidase S, removes terminal galactose residues from bovine fetuin's glycans in vitro[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
≥1000U/mL
One unit is defined as the amount of enzyme that releases 1 nmol of galactose per minute from Gal β1-3GlcNAcβ1-3Galβ1-4Glc at 37°C, pH 5.0
Chemical Information
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CAS No. 9031-11-2
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Appearance Liquid (Density: 1.14 g/cm3)
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Color Colorless to light yellow
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SMILES
[b1-3 Galactosidase]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Viborg AH, et al. A β1-6/β1-3 galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 gives insight into sub-specificities of β-galactoside catabolism within Bifidobacterium. Mol Microbiol. Published online October 7, 2014. [Content Brief]
[2]. Saunders MJ, et al. Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering. Sci Rep. 2023;13(1):567. Published 2023 Jan 11. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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