β1-3,4,6 Galactosidase
Based on 1 Customer Validation
β1-3,4,6 Galactosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β1-3 β1-4 and β1-6 linked galactose residues from oligosaccharides, with β1-6 linked galactose residues at a slower rate.
For research use only. We do not sell to patients.
- CAS No.: 9031-11-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
others
In Vitro
Product Information
This product is expressed by recombinant E. coli. The buffer composition is 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol. It can be used to release β(1→3)-, β(1→4) and β(1→6)-linked galactose from the non-reducing end of complex oligosaccharides.
Instructions
1. Add substrate according to the reaction system, 50 mM sodium acetate, 5 mM CaCl2, pH 5.0, mix well, and add appropriate amount of β1-3,4,6-galactosidase (according to the reaction requirements);
2. After mixing, incubate the sample at 37°C for 2-4 h.
Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and low-speed shaking can be used for mixing.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Enzyme Activity
>2000U/mL
Unit Definition
One unit is defined as the amount of enzyme that releases 1 nmol of galactose per minute from Gal β1-3 Glcnacβ1-3 Galβ1-4GLC at 37 °C, pH 5.0.
Chemical Information
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CAS No. 9031-11-2
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[b1-3,4,6 Galactosidase]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
β1-3,4,6 Galactosidase Related Classifications
HY-P2869E Related Classifications
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