BCECF
Based on 3 publication(s) in Google Scholar
BCECF is a cell-impermeant pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
For research use only. We do not sell to patients.
- Purity: 94.0%
- CAS No.: 85138-49-4
- Formula: C54H40O22
- Molecular Weight:520.44
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BCECF
More
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare a 2-20 mM stock solution of BCECF in DMSO.
2. Prepare a 5-50 μM BCECF dye solution in buffer solutions (Hepes, pH 7.4 or PBS) (BCECF can be introduced into cells using methods such as patch clamping and microinjection.).
3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.
4. Incubate the dye-loading plate in a cell incubator about 30minutes.
5. Ratio measurement using dual-wavelength excitation:
Ex/Em = 490 nm / 535 nm (pH-sensitive wavelength)
Ex/Em = 440 nm / 535 nm (pH equal extinction point, used as a reference).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
528
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Chemical Information
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CAS No. 85138-49-4
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Appearance Solid
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Molecular Weight 520.44
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Formula C54H40O22
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Color Yellow to orange
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SMILES
O=C(O)CCC1=CC(C23OC(C4=C2C=CC(C(O)=O)=C4)=O)=C(OC5=C3C=C(CCC(O)=O)C(O)=C5)C=C1O.O=C(O)CCC6=CC(C78OC(C9=C7C=C(C(O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(O)=O)C(O)=C%10)C=C6O
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Synonyms
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Rep Med
pH-regulating Lipiodol Pickering emulsions enhance transarterial embolization therapy via inducing ferroptosis and activating antitumor immunity. [Abstract]2026 Mar 17;7(3):102662. PMID: 41850231 -
Cell Rep
Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. [Abstract]2025 Jul 15;44(7):116024. PMID: 40668679 -
Microorganisms
Differences in Acid Stress Response of Lacticaseibacillus paracasei Zhang Cultured from Solid-State Fermentation and Liquid-State Fermentation. [Abstract]2021 Sep 14;9(9):1951. PMID: 34576848
Solvent & Solubility
DMSO : 62.5 mg/mL (120.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (286 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9215 mL | 9.6073 mL | 19.2145 mL | 48.0363 mL |
| 5 mM | 0.3843 mL | 1.9215 mL | 3.8429 mL | 9.6073 mL | |
| 10 mM | 0.1921 mL | 0.9607 mL | 1.9215 mL | 4.8036 mL | |
| 15 mM | 0.1281 mL | 0.6405 mL | 1.2810 mL | 3.2024 mL | |
| 20 mM | 0.0961 mL | 0.4804 mL | 0.9607 mL | 2.4018 mL | |
| 25 mM | 0.0769 mL | 0.3843 mL | 0.7686 mL | 1.9215 mL | |
| 30 mM | 0.0640 mL | 0.3202 mL | 0.6405 mL | 1.6012 mL | |
| 40 mM | 0.0480 mL | 0.2402 mL | 0.4804 mL | 1.2009 mL | |
| 50 mM | 0.0384 mL | 0.1921 mL | 0.3843 mL | 0.9607 mL | |
| 60 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8006 mL | |
| 80 mM | 0.0240 mL | 0.1201 mL | 0.2402 mL | 0.6005 mL | |
| 100 mM | 0.0192 mL | 0.0961 mL | 0.1921 mL | 0.4804 mL |
BCECF Related Classifications
HY-101882 Related Classifications
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