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  3. Fluorescent Dye
  4. BCECF

BCECF  (Synonyms: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein)

Cat. No.: HY-101882 Purity: ≥94.0%
COA Handling Instructions

Molarity Calculator

BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

For research use only. We do not sell to patients.

BCECF Chemical Structure

BCECF Chemical Structure

CAS No. : 85138-49-4

Size Price Stock Quantity
1 mg USD 65 In-stock
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10 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

BCECF Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BCECF

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare a 2-20 mM stock solution of BCECF in DMSO and store at -20℃.
2. Prepare a 5-50 μM BCECF dye-loading solution in buffer solutions (Hepes, pH 7.4 or PBS).
3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.
4. Incubate the dye-loading plate in a cell incubator about 30minutes.
5. Wash and replace the dye-loading solution with buffers.
6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BCECF Related Antibodies
Molecular Weight

520.44

Formula

C54H40O22
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

528
Excitation (Ex)

503
SMILES

O=C(O)CCC1=CC(C23OC(C4=C2C=CC(C(O)=O)=C4)=O)=C(OC5=C3C=C(CCC(O)=O)C(O)=C5)C=C1O.O=C(O)CCC6=CC(C78OC(C9=C7C=C(C(O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(O)=O)C(O)=C%10)C=C6O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (120.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9215 mL 9.6073 mL 19.2145 mL
5 mM 0.3843 mL 1.9215 mL 3.8429 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥96.0%

COA (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9215 mL 9.6073 mL 19.2145 mL 48.0363 mL
5 mM 0.3843 mL 1.9215 mL 3.8429 mL 9.6073 mL
10 mM 0.1921 mL 0.9607 mL 1.9215 mL 4.8036 mL
15 mM 0.1281 mL 0.6405 mL 1.2810 mL 3.2024 mL
20 mM 0.0961 mL 0.4804 mL 0.9607 mL 2.4018 mL
25 mM 0.0769 mL 0.3843 mL 0.7686 mL 1.9215 mL
30 mM 0.0640 mL 0.3202 mL 0.6405 mL 1.6012 mL
40 mM 0.0480 mL 0.2402 mL 0.4804 mL 1.2009 mL
50 mM 0.0384 mL 0.1921 mL 0.3843 mL 0.9607 mL
60 mM 0.0320 mL 0.1601 mL 0.3202 mL 0.8006 mL
80 mM 0.0240 mL 0.1201 mL 0.2402 mL 0.6005 mL
100 mM 0.0192 mL 0.0961 mL 0.1921 mL 0.4804 mL
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BCECF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCECF85138-49-42′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluoresceinFluorescent DyepH-sensitiveInhibitorinhibitorinhibit

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