2. Others
  3. Fluorescent Dye
  4. BCECF

BCECF  (Synonyms: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein)

Cat. No.: HY-101882 Purity: 94.0%
COA
SDS
Handling Instructions Technical Support

BCECF is a cell-impermeant pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

For research use only. We do not sell to patients.

BCECF

BCECF Chemical Structure

CAS No. : 85138-49-4

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of BCECF:

BCECF Related Antibodies

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Description

BCECF is a cell-impermeant pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare a 2-20 mM stock solution of BCECF in DMSO.
2. Prepare a 5-50 μM BCECF dye solution in buffer solutions (Hepes, pH 7.4 or PBS) (BCECF can be introduced into cells using methods such as patch clamping and microinjection.).
3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.
4. Incubate the dye-loading plate in a cell incubator about 30minutes.
5. Ratio measurement using dual-wavelength excitation:
Ex/Em = 490 nm / 535 nm (pH-sensitive wavelength)
Ex/Em = 440 nm / 535 nm (pH equal extinction point, used as a reference).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BCECF Related Antibodies
Molecular Weight

520.44

Formula

C54H40O22
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

528
Excitation (Ex)

503
SMILES

O=C(O)CCC1=CC(C23OC(C4=C2C=CC(C(O)=O)=C4)=O)=C(OC5=C3C=C(CCC(O)=O)C(O)=C5)C=C1O.O=C(O)CCC6=CC(C78OC(C9=C7C=C(C(O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(O)=O)C(O)=C%10)C=C6O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (120.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9215 mL 9.6073 mL 19.2145 mL
5 mM 0.3843 mL 1.9215 mL 3.8429 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥96.0%

SDS (252 KB)

COA (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9215 mL 9.6073 mL 19.2145 mL 48.0363 mL
5 mM 0.3843 mL 1.9215 mL 3.8429 mL 9.6073 mL
10 mM 0.1921 mL 0.9607 mL 1.9215 mL 4.8036 mL
15 mM 0.1281 mL 0.6405 mL 1.2810 mL 3.2024 mL
20 mM 0.0961 mL 0.4804 mL 0.9607 mL 2.4018 mL
25 mM 0.0769 mL 0.3843 mL 0.7686 mL 1.9215 mL
30 mM 0.0640 mL 0.3202 mL 0.6405 mL 1.6012 mL
40 mM 0.0480 mL 0.2402 mL 0.4804 mL 1.2009 mL
50 mM 0.0384 mL 0.1921 mL 0.3843 mL 0.9607 mL
60 mM 0.0320 mL 0.1601 mL 0.3202 mL 0.8006 mL
80 mM 0.0240 mL 0.1201 mL 0.2402 mL 0.6005 mL
100 mM 0.0192 mL 0.0961 mL 0.1921 mL 0.4804 mL
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BCECF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCECF85138-49-42′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluoresceinFluorescent DyepH-sensitiveInhibitorinhibitorinhibit

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