2. Cell Cycle/DNA Damage Stem Cell/Wnt
  3. Casein Kinase Wnt
  4. SSTC3

SSTC3 is a casein kinase 1α (CK1α) activator (Kd = 32 nM) that inhibits WNT signaling (EC50 = 30 nM). SSTC3 exhibits minimal gastrointestinal toxicity compared to other classes of WNT inhibitors.

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SSTC3

SSTC3 構造式

CAS 番号 : 1242422-09-8

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 342 在庫あり
Solution
10 mM * 1 mL in DMSO USD 342 在庫あり
Solid
5 mg $300 在庫あり
10 mg $480 在庫あり
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100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 2 publication(s) in Google Scholar

SSTC3 関連抗体

Top Publications Citing Use of Products

Casein Kinase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
CK1 CK2

Wnt アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Wnt1 Wnt2 Wnt3 Wnt5 Wnt7 Wnt11

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  • 純度とドキュメンテーション

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製品説明

SSTC3 is a casein kinase 1α (CK1α) activator (Kd = 32 nM) that inhibits WNT signaling (EC50 = 30 nM). SSTC3 exhibits minimal gastrointestinal toxicity compared to other classes of WNT inhibitors[1].

Cellular Effect
Cell Line Type Value Description References
HCT-116 EC50
123 nM
Compound: 101; SSTC3
Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability
Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability
[PMID: 33822624]
HT-29 EC50
132 nM
Compound: 101; SSTC3
Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability
Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability
[PMID: 33822624]
体外実験

SSTC3 (0-1μM, 5 days) decreases the viability of HCT116 cells in an on-target manner. This capacity is significantly reduced when the mutant CTNNB1 allele driving its carcinogenic properties is deleted[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SSTC3 関連抗体

Cell Viability Assay[1]

Cell Line: Colorectal cancer (CRC) cell lines.
Concentration: 0-1 μM.
Incubation Time: 5 days.
Result: Decreased the viability.

Western Blot Analysis[1]

Cell Line: SW403 cells.
Concentration: 100 nM.
Incubation Time: 15 min.
Result: Increased β-catenin phosphate levels.
体内実験

SSTC3 can be maintained for 24 hours after treatment[1].
SSTC3 (25 mg/kg, ip once daily for 8-12 days) suppresses the growth of colorectal carcinoma in CD-1 mice[1].
SSTC3 (10 mg/kg, ip once daily for 1 month) inhibits the growth of Apc mutation-driven tumors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Five-week-old Apcmin mice[1].
Dosage: 10 mg/kg.
Administration: IP for 1 month.
Result: Inhibited the growth of Apc mutation-driven tumors.
Animal Model: CD-1 mice.
Dosage: 25 mg/kg.
Administration: IP once daily for 8-12 days.
Result: Inhibited the growth of HCT116 xenografts.
Attenuated the growth of this metastatic CRC PDX and markedly reduced the cell density of residual cancer.
Reduced the expression of WNT biomarkers in this CRC PDX.
分子量

518.53

分子式

C23H17F3N4O3S2
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=NC(C2=NC=CC=C2)=CS1)C3=CC=C(S(=O)(N(C)C4=CC=C(C(F)(F)F)C=C4)=O)C=C3
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 125 mg/mL (241.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9285 mL 9.6426 mL 19.2853 mL
5 mM 0.3857 mL 1.9285 mL 3.8571 mL
10 mM 0.1929 mL 0.9643 mL 1.9285 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9285 mL 9.6426 mL 19.2853 mL 48.2132 mL
5 mM 0.3857 mL 1.9285 mL 3.8571 mL 9.6426 mL
10 mM 0.1929 mL 0.9643 mL 1.9285 mL 4.8213 mL
15 mM 0.1286 mL 0.6428 mL 1.2857 mL 3.2142 mL
20 mM 0.0964 mL 0.4821 mL 0.9643 mL 2.4107 mL
25 mM 0.0771 mL 0.3857 mL 0.7714 mL 1.9285 mL
30 mM 0.0643 mL 0.3214 mL 0.6428 mL 1.6071 mL
40 mM 0.0482 mL 0.2411 mL 0.4821 mL 1.2053 mL
50 mM 0.0386 mL 0.1929 mL 0.3857 mL 0.9643 mL
60 mM 0.0321 mL 0.1607 mL 0.3214 mL 0.8036 mL
80 mM 0.0241 mL 0.1205 mL 0.2411 mL 0.6027 mL
100 mM 0.0193 mL 0.0964 mL 0.1929 mL 0.4821 mL
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SSTC3 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SSTC31242422-09-8SSTC 3SSTC-3Casein KinaseWntcolorectalcancerCTNNB1HCT116CRCSW403lowtoxicityInhibitorinhibitorinhibit

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SSTC3
製品番号:
HY-120675
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