2. GPCR/G Protein Neuronal Signaling Autophagy PI3K/Akt/mTOR
  3. Adrenergic Receptor Atg8/LC3 Akt mTOR
  4. Tetrahydroalstonine

Tetrahydroalstonine  (Synonyms: (-)-Tetrahydroalstonine)

Cat. No.: HY-N1163 Purity: 99.95%
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Tetrahydroalstonine ((-)-Tetrahydroalstonine) is an indole alkaloid and a selective α₂-adrenergic receptor antagonist. Tetrahydroalstonine exhibits certain neuroprotective effects. Tetrahydroalstonine can regulate autophagy-lysosomal function by activating the Akt/mTOR pathway, significantly reducing OGD/R-induced primary cortical neuronal injury.

For research use only. We do not sell to patients.

Tetrahydroalstonine Chemical Structure

Tetrahydroalstonine Chemical Structure

CAS No. : 6474-90-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
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Based on 1 publication(s) in Google Scholar

Tetrahydroalstonine Related Antibodies

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Description

Tetrahydroalstonine ((-)-Tetrahydroalstonine) is an indole alkaloid and a selective α₂-adrenergic receptor antagonist. Tetrahydroalstonine exhibits certain neuroprotective effects. Tetrahydroalstonine can regulate autophagy-lysosomal function by activating the Akt/mTOR pathway, significantly reducing OGD/R-induced primary cortical neuronal injury[1][2][3].

In Vitro

Tetrahydroalstonine (0.75-3 µM; 3-26 h) improves cell viability and ameliorates abnormal expression of autophagic and lysosomal markers (LC3B-II/I, LAMP1, CSTB, TFEB) in primary cortical neurons treated with oxygen-glucose deprivation/reoxygenation (OGD/R). The mechanism involves activation of the Akt/mTOR pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tetrahydroalstonine Related Antibodies

Cell Viability Assay[1]

Cell Line: OGD/R treated cortical neurons
Concentration: 0.75, 1.5 and 3 µM
Incubation Time: Pretreated with 2 h, then co-incubation for 24 h
Result: Increased the cell viability of cortical neurons.
In Vivo

Tetrahydroalstonine (0.5-4 mg/kg; intravenous injection; single dose) in pithed rats shows that low doses (0.5-2 mg/kg) enhance the pressor response induced by electrical stimulation of sympathetic nerves, while high dose (4 mg/kg) inhibits the response[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats were pithed and artificially ventilated with vagus nerve sectioning
Dosage: 0.5, 1, 2 and 4 mg/kg
Administration: Intravenous injection (i.v.); single dose
Result: Significantly potentiated the pressor response to electrical stimulation of sympathetic outflow (shown as negative inhibition) at low doses (0.5-2 mg/kg), while 4 mg/kg significantly reduced this response (PN50: 2.8 mg/kg). The potentiation at low doses suggested blockade of presynaptic α₂-adrenoceptors, leading to increased noradrenaline release.
Molecular Weight

352.43

Formula

C21H24N2O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(OC)C1=CO[C@@H](C)[C@@]2([H])[C@]1([H])C[C@@]3([H])C4=C(C5=CC=CC=C5N4)CCN3C2
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (283.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8374 mL 14.1872 mL 28.3744 mL
5 mM 0.5675 mL 2.8374 mL 5.6749 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8374 mL 14.1872 mL 28.3744 mL 70.9361 mL
5 mM 0.5675 mL 2.8374 mL 5.6749 mL 14.1872 mL
10 mM 0.2837 mL 1.4187 mL 2.8374 mL 7.0936 mL
15 mM 0.1892 mL 0.9458 mL 1.8916 mL 4.7291 mL
20 mM 0.1419 mL 0.7094 mL 1.4187 mL 3.5468 mL
25 mM 0.1135 mL 0.5675 mL 1.1350 mL 2.8374 mL
30 mM 0.0946 mL 0.4729 mL 0.9458 mL 2.3645 mL
40 mM 0.0709 mL 0.3547 mL 0.7094 mL 1.7734 mL
50 mM 0.0567 mL 0.2837 mL 0.5675 mL 1.4187 mL
60 mM 0.0473 mL 0.2365 mL 0.4729 mL 1.1823 mL
80 mM 0.0355 mL 0.1773 mL 0.3547 mL 0.8867 mL
100 mM 0.0284 mL 0.1419 mL 0.2837 mL 0.7094 mL
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Tetrahydroalstonine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetrahydroalstonine6474-90-4(-)-TetrahydroalstonineAdrenergic ReceptorAtg8/LC3AktmTORBeta ReceptorAutophagy-related 8PKBProtein kinase BMammalian target of RapamycinIndolealkaloidRhazyastrictaalpha2-adrenoceptorcortical neuronsInhibitorinhibitorinhibit

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