TP-α  (Synonyms: TPG-456)

TP-α is a glucagon-selective two-photon fluorescent probe with a K_d of 65 μM. TP-α directly interacts with glucagon to produce significant fluorescence enhancement. TP-α selectively stains viable glucagon-secreting α cells in pancreatic islets, enabling visualization of their localization, distribution and availability. TP-α is applicable to diabetes research^[1].

TP-α (10 μM; 30 min on ice) binds directly and selectively to glucagon with a K_d of 65 μM, showing a 9-fold fluorescence increase at 1 mg/mL glucagon^[1].
TP-α (0.5-1 μM; 1 h) selectively stains glucagon-secreting alpha TC1 cells, with negligible fluorescence in beta TC6 and acinar 266-6 cells^[1].
TP-α (1-2 μM; 1, 24 h) has minimal cytotoxicity toward alpha TC1 and beta TC6 cells after incubation for 1 or 24 h^[1].
TP-α (1 μM for one-photon; 10 μM for two-photon) exhibits one-photon absorption/emission at 370/510 nm (quantum yield 0.69) and a two-photon action cross section of 107 GM, with optimal two-photon excitation at 750 nm and emission at 430-530 nm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

581.83

C₃₈H₅₁N₃O₂

1708978-54-4

CC(C1=CC2=C(C3(CCCC(CN(C(CN(C)C4=CC5=CC=C(C=C5C=C4)C(C)=O)=O)CCCN)(C3CC2)C)C)C=C1)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Agrawalla BK, et al. Glucagon-Secreting Alpha Cell Selective Two-Photon Fluorescent Probe TP-α: For Live Pancreatic Islet Imaging. J Am Chem Soc. 2015;137(16):5355-5362.  [Content Brief]