DS02312223
Based on 1 Customer Validation
DS02312223 (D223) is a molecular glue that promotes the binding of RAS to PI3Kα, with a Kd of 0.76 μM for p110α. DS02312223 increases the binding affinity between GTP-bound KRAS (KRAS-GMPPNP) and p110α by nearly three orders of magnitude (KD = 0.017 μM). DS02312223 stimulates the translocation of GLUT4 to the plasma membrane. DS02312223 promotes glucose uptake in the absence of insulin. DS02312223 can be used in diabetes research.
For research use only. We do not sell to patients.
- Purity: 98.24%
- CAS No.: 2253733-45-6
- Formula: C29H32FN5O5S
- Molecular Weight:581.66
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
|
PI3Kα |
GLUT4 |
DS02312223 (10-10000 nM) stimulates concentration-dependent GLUT4 translocation in differentiated L6-GLUT4 myc myotubes in the absence of insulin[1].
DS02312223 (1 μM; 5 min) enhances the interaction between PI3Kα and RRAS2 in serum-starved L6-GLUT4myc myotubes[1].
DS02312223 binds directly to p110α (KD = 0.76 μM) and increases the binding affinity between GTP-bound KRAS (KRAS-GMPPNP) and p110α by nearly three orders of magnitude (KD = 0.017 μM), but does not bind KRAS-GMPPNP or induce binding between KRAS-GDP and p110α[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:L6-GLUT4 myc myotubes
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Concentration:1 μM
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Incubation Time:5 min
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Result:Increased the association of PI3Kα with RRAS2, whereas the inactive compound D484 had no effect.
Chemical Information
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CAS No. 2253733-45-6
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Appearance Solid
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Molecular Weight 581.66
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Formula C29H32FN5O5S
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Color White to off-white
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SMILES
CC(C)(OC(NCCOCCOC1=C(C=CC=C1)C2=C3SC=CC3=C(N=N2)NC4=C(C=C(C=C4)CC(N)=O)F)=O)C
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Synonyms
D223
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (171.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7192 mL | 8.5961 mL | 17.1922 mL | 42.9804 mL |
| 5 mM | 0.3438 mL | 1.7192 mL | 3.4384 mL | 8.5961 mL | |
| 10 mM | 0.1719 mL | 0.8596 mL | 1.7192 mL | 4.2980 mL | |
| 15 mM | 0.1146 mL | 0.5731 mL | 1.1461 mL | 2.8654 mL | |
| 20 mM | 0.0860 mL | 0.4298 mL | 0.8596 mL | 2.1490 mL | |
| 25 mM | 0.0688 mL | 0.3438 mL | 0.6877 mL | 1.7192 mL | |
| 30 mM | 0.0573 mL | 0.2865 mL | 0.5731 mL | 1.4327 mL | |
| 40 mM | 0.0430 mL | 0.2149 mL | 0.4298 mL | 1.0745 mL | |
| 50 mM | 0.0344 mL | 0.1719 mL | 0.3438 mL | 0.8596 mL | |
| 60 mM | 0.0287 mL | 0.1433 mL | 0.2865 mL | 0.7163 mL | |
| 80 mM | 0.0215 mL | 0.1075 mL | 0.2149 mL | 0.5373 mL | |
| 100 mM | 0.0172 mL | 0.0860 mL | 0.1719 mL | 0.4298 mL |
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