2,6-Dichlorophenolindophenol sodium hydrate
Based on 1 publication(s) in Google Scholar
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae.
For research use only. We do not sell to patients.
- CAS No.: 1266615-56-8
- Formula: C12H6Cl2NNaO2.xH2O
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 2,6-Dichlorophenolindophenol sodium hydrate
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Biological Activity
Incubation of Saccharomyces cerevisiae with 2,6-Dichlorophenolindophenol sodium hydrate (75 μM; 10 min) for 10 min at pH 6.0-7.0 yields the maximum spectrophotometric BOD response, and a stable response is observable over the pH range of 7.0 to 9.0[1].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) acts as a redox chromogenic indicator for the metabolic activity of Saccharomyces cerevisiae. When incubated at 30 °C for 10 min, its absorbance decreases linearly with glucose concentrations ranging from 0 to 4.5 mg/L, with a correlation coefficient of 0.999[2].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) enables the detection of metabolic inhibition of Saccharomyces cerevisiae by Cu2+ and Mn2+ via the reduction of colorimetric response, whereas most coexisting inorganic ions, anionic surfactants and other tested toxicants exert minimal interference with this assay under the condition of incubation at 30 °C for 10 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1266615-56-8
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Appearance Solid
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Formula C12H6Cl2NNaO2.xH2O
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Color Green to dark green
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SMILES
O=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(C=C2)O[Na].O.[x]
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Synonyms
DCIP sodium hydrate; DPIP sodium hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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EMBO Mol Med
De novo pyrimidine synthesis is a collateral metabolic vulnerability in NF2-deficient mesothelioma. [Abstract]2025 Jul 24. PMID: 40707702
Solvent & Solubility
DMSO : 35 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Nakamura H, et al. A spectrophotometric biochemical oxygen demand determination method using 2,6-dichlorophenolindophenol as the redox color indicator and the eukaryote Saccharomyces cerevisiae. Anal Biochem. 2007;369(2):168-174. [Content Brief]
[2]. Nakamura H, et al. A simple and highly repeatable colorimetric toxicity assay method using 2,6-dichlorophenolindophenol as the redox color indicator and whole eukaryote cells. Anal Bioanal Chem. 2007;389(3):835-840. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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