2,6-Dichlorophenolindophenol sodium hydrate
Based on 1 publication(s) in Google Scholar
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae.
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- No. CAS: 1266615-56-8
- Fòrmula: C12H6Cl2NNaO2.xH2O
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Almacenamiento:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) 2,6-Dichlorophenolindophenol sodium hydrate
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Actividad biológica
Descripciòn
In Vitro
Incubation of Saccharomyces cerevisiae with 2,6-Dichlorophenolindophenol sodium hydrate (75 μM; 10 min) for 10 min at pH 6.0-7.0 yields the maximum spectrophotometric BOD response, and a stable response is observable over the pH range of 7.0 to 9.0[1].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) acts as a redox chromogenic indicator for the metabolic activity of Saccharomyces cerevisiae. When incubated at 30 °C for 10 min, its absorbance decreases linearly with glucose concentrations ranging from 0 to 4.5 mg/L, with a correlation coefficient of 0.999[2].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) enables the detection of metabolic inhibition of Saccharomyces cerevisiae by Cu2+ and Mn2+ via the reduction of colorimetric response, whereas most coexisting inorganic ions, anionic surfactants and other tested toxicants exert minimal interference with this assay under the condition of incubation at 30 °C for 10 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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No. CAS 1266615-56-8
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Appearance Solid
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Fòrmula C12H6Cl2NNaO2.xH2O
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Color Green to dark green
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SMILES
O=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(C=C2)O[Na].O.[x]
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Synonyms
DCIP sodium hydrate; DPIP sodium hydrate
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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EMBO Mol Med
De novo pyrimidine synthesis is a collateral metabolic vulnerability in NF2-deficient mesothelioma. [Abstract]2025 Jul 24. PMID: 40707702
Solvente y solubilidad
In Vitro:
DMSO : 35 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Protocolo
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Nakamura H, et al. A spectrophotometric biochemical oxygen demand determination method using 2,6-dichlorophenolindophenol as the redox color indicator and the eukaryote Saccharomyces cerevisiae. Anal Biochem. 2007;369(2):168-174. [Content Brief]
[2]. Nakamura H, et al. A simple and highly repeatable colorimetric toxicity assay method using 2,6-dichlorophenolindophenol as the redox color indicator and whole eukaryote cells. Anal Bioanal Chem. 2007;389(3):835-840. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2,6-Dichlorophenolindophenol sodium hydrate Related Classifications
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