2,6-Dichlorophenolindophenol sodium hydrate  (Synonyms: DCIP sodium hydrate; DPIP sodium hydrate)

2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae^[1][2].

Incubation of Saccharomyces cerevisiae with 2,6-Dichlorophenolindophenol sodium hydrate (75 μM; 10 min) for 10 min at pH 6.0-7.0 yields the maximum spectrophotometric BOD response, and a stable response is observable over the pH range of 7.0 to 9.0^[1].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) acts as a redox chromogenic indicator for the metabolic activity of Saccharomyces cerevisiae. When incubated at 30 °C for 10 min, its absorbance decreases linearly with glucose concentrations ranging from 0 to 4.5 mg/L, with a correlation coefficient of 0.999^[2].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) enables the detection of metabolic inhibition of Saccharomyces cerevisiae by Cu²⁺ and Mn²⁺ via the reduction of colorimetric response, whereas most coexisting inorganic ions, anionic surfactants and other tested toxicants exert minimal interference with this assay under the condition of incubation at 30 °C for 10 min^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C₁₂H₆Cl₂NNaO₂.xH₂O

1266615-56-8

Solid

Green to dark green

O=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(C=C2)O[Na].O.[x]

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

• [1]. Nakamura H, et al. A spectrophotometric biochemical oxygen demand determination method using 2,6-dichlorophenolindophenol as the redox color indicator and the eukaryote Saccharomyces cerevisiae. Anal Biochem. 2007;369(2):168-174.

  [2]. Nakamura H, et al. A simple and highly repeatable colorimetric toxicity assay method using 2,6-dichlorophenolindophenol as the redox color indicator and whole eukaryote cells. Anal Bioanal Chem. 2007;389(3):835-840.