2,6-Dichlorophenolindophenol sodium hydrate
Based on 1 publication(s) in Google Scholar
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae.
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- CAS No.: 1266615-56-8
- Formule: C12H6Cl2NNaO2.xH2O
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Stockage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Publications Citing Use of MedChemExpress (MCE) 2,6-Dichlorophenolindophenol sodium hydrate
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Activité biologique
Description
In Vitro
Incubation of Saccharomyces cerevisiae with 2,6-Dichlorophenolindophenol sodium hydrate (75 μM; 10 min) for 10 min at pH 6.0-7.0 yields the maximum spectrophotometric BOD response, and a stable response is observable over the pH range of 7.0 to 9.0[1].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) acts as a redox chromogenic indicator for the metabolic activity of Saccharomyces cerevisiae. When incubated at 30 °C for 10 min, its absorbance decreases linearly with glucose concentrations ranging from 0 to 4.5 mg/L, with a correlation coefficient of 0.999[2].
2,6-Dichlorophenolindophenol sodium hydrate (750 μM; 10 min) enables the detection of metabolic inhibition of Saccharomyces cerevisiae by Cu2+ and Mn2+ via the reduction of colorimetric response, whereas most coexisting inorganic ions, anionic surfactants and other tested toxicants exert minimal interference with this assay under the condition of incubation at 30 °C for 10 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1266615-56-8
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Appearance Solid
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Formule C12H6Cl2NNaO2.xH2O
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Color Green to dark green
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SMILES
O=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(C=C2)O[Na].O.[x]
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Synonyms
DCIP sodium hydrate; DPIP sodium hydrate
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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EMBO Mol Med
De novo pyrimidine synthesis is a collateral metabolic vulnerability in NF2-deficient mesothelioma. [Abstract]2025 Jul 24. PMID: 40707702
Solvant et solubilité
In Vitro:
DMSO : 35 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Pureté et documentation
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Fiche technique (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Nakamura H, et al. A spectrophotometric biochemical oxygen demand determination method using 2,6-dichlorophenolindophenol as the redox color indicator and the eukaryote Saccharomyces cerevisiae. Anal Biochem. 2007;369(2):168-174. [Content Brief]
[2]. Nakamura H, et al. A simple and highly repeatable colorimetric toxicity assay method using 2,6-dichlorophenolindophenol as the redox color indicator and whole eukaryote cells. Anal Bioanal Chem. 2007;389(3):835-840. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2,6-Dichlorophenolindophenol sodium hydrate Related Classifications
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